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Development of a novel genetic assay for telomere recombination in Saccharomyces cerevisiae
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 Title & Authors
Development of a novel genetic assay for telomere recombination in Saccharomyces cerevisiae
Kim, Min-Kyu; Bae, Sung-Ho;
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Stable maintenance of telomere is required for cell proliferation and survival. Although telomerase is the primary means for telomere maintenance, recombination is another important pathway to maintain telomeres. In this study, we developed a genetic assay for telomere recombination using the internal repeats present in subtelomeric regions of yeast. The recombination frequencies were dependent on the presence of the internal repeats. PCR amplification of the regions near URA3 and CAN1 markers using genomic DNA isolated from colonies indicated that each isolate had lost the chromosome end including the markers. In addition, the recombination frequencies increased with longer internal repeats. Our results suggest that the colony formation is the consequence of recombination between the internal and terminal repeats.
assay for telomere recombination;telomere;telomere recombination;yeast telomere;
 Cited by
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