Advanced SearchSearch Tips
Succession of bacterial community structure during the early stage of biofilm development in the Antarctic marine environment
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Succession of bacterial community structure during the early stage of biofilm development in the Antarctic marine environment
Lee, Yung Mi; Cho, Kyung Hee; Hwang, Kyuin; Kim, Eun Hye; Kim, Mincheol; Hong, Soon Gyu; Lee, Hong Kum;
  PDF(new window)
Compared to planktonic bacterial populations, biofilms have distinct bacterial community structures and play important ecological roles in various aquatic environments. Despite their ecological importance in nature, bacterial community structure and its succession during biofilm development in the Antarctic marine environment have not been elucidated. In this study, the succession of bacterial community, particularly during the early stage of biofilm development, in the Antarctic marine environment was investigated by pyrosequencing of the 16S rRNA gene. Overall bacterial distribution in biofilms differed considerably from surrounding seawater. Relative abundance of Gammaproteobacteria and Bacteroidetes which accounted for 78.9-88.3% of bacterial community changed drastically during biofilm succession. Gammaproteobacteria became more abundant with proceeding succession (75.7% on day 4) and decreased to 46.1% on day 7. The relative abundance of Bacteroidetes showed opposite trend to Gammaproteobacteria, decreasing from the early days to the intermediate days and becoming more abundant in the later days. There were striking differences in the composition of major OTUs () among samples during the early stages of biofilm formation. Gammaproteobacterial species increased until day 4, while members of Bacteroidetes, the most dominant group on day 1, decreased until day 4 and then increased again. Interestingly, Pseudoalteromonas prydzensis was predominant, accounting for up to 67.4% of the biofilm bacterial community and indicating its important roles in the biofilm development.
Bacteroidetes;Pseudoalteromonas;Antarctica;biofilm succession;bacterial community;pyrosequencing;
 Cited by
Draft Genome Sequence of Marinobacter vinifirmus Type Strain FB1, Genome Announcements, 2017, 5, 39, e01058-17  crossref(new windwow)
Early bacterial biofilm colonizers in the coastal waters of Mauritius, Electronic Journal of Biotechnology, 2017, 29, 13  crossref(new windwow)
Influence of Darkness and Aging on Marine and Freshwater Biofilm Microbial Communities Using Microcosm Experiments, Microbial Ecology, 2018  crossref(new windwow)
Abell, G.C.J. and Bowman, J.P. 2005. Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean. FEMS Microbiol. Ecol. 51, 265-277. crossref(new window)

Araya, R., Tani, K., Takagi, T., Yamaguchi, N., and Nasu, M. 2003. Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis. FEMS Microbiol. Ecol. 43, 111-119. crossref(new window)

Armstrong, E., Yan, L., Boyd, K.G., Wright, P.C., and Burgess, J.G. 2001. The symbiotic role of marine microbes on living surfaces. Hydrobiologia 461, 37-40. crossref(new window)

Bowman, J.P. 1998. Pseudoalteromonas prydzensis sp. nov., a psychrotrophic, halotolerant bacterium from antarctic sea ice. Int. J. Syst. Evol. Microbiol. 48, 1037-1041.

Bowman, J.P. 2007. Bioactive compound synthetic capacity and ecological significance of marine bacterial genus Pseudoalteromonas. Mar. Drugs 5, 220-241. crossref(new window)

Costerton, J.W., Stewart, P.S., and Greenberg, E.P. 1999. Bacterial biofilms: a common cause of persistent infections. Science 284, 1318-1322. crossref(new window)

Dang, H. and Lovell, C.R. 2000. Bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rRNA gene restriction analysis and sequence analysis of 16S rRNA genes. Appl. Environ. Microbiol. 66, 467-475. crossref(new window)

Decho, A. 2000. Exopolymer microdomains as a structuring agent for heterogeneity within microbial biofilms, pp. 9-15. In Riding, R. and Awramik, S. (eds.), Microbial sediments. Springer Berlin Heidelberg.

Delille, D. 1996. Biodiversity and function of bacteria in the southern ocean. Biodivers. Conserv. 5, 1505-1523. crossref(new window)

Donlan, R.M. 2002. Biofilms: Microbial life on surfaces. Emerg. Infect. Dis. 8, 881-890. crossref(new window)

Edgar, R.C., Haas, B.J., Clemente, J.C., Quince, C., and Knight, R. 2011. Uchime improves sensitivity and speed of chimera detection. Bioinformatics 27, 2194-2200. crossref(new window)

Egan, S., Thomas, T., and Kjelleberg, S. 2008. Unlocking the diversity and biotechnological potential of marine surface associated microbial communities. Curr. Opin. Microbiol. 11, 219-225. crossref(new window)

Field, K.G., Gordon, D., Wright, T., Rappe, M., Urback, E., Vergin, K., and Giovannoni, S.J. 1997. Diversity and depth-specific distribution of sar11 cluster rRNA genes from marine planktonic bacteria. Appl. Environ. Microbiol. 63, 63-70.

Flemming, H.C. 2002. Biofouling in water systems - cases, causes and countermeasures. Appl. Microbiol. Biotechnol. 59, 629-640. crossref(new window)

Gaylarde, C.C. and Morton, L.H.G. 1999. Deteriogenic biofilms on buildings and their control: a review. Biofouling 14, 59-74. crossref(new window)

Gillan, D.C., Speksnijder, A.G.C.L., Zwart, G., and De Ridder, C. 1998. Genetic diversity of the biofilm covering Montacuta ferruginosa (Mollusca, Bivalvia) as evaluated by denaturing gradient gel electrophoresis analysis and cloning of PCR-amplified gene fragments coding for 16S rRNA. Appl. Environ. Microbiol. 64, 3464-3472.

Hamady, M., Lozupone, C., and Knight, R. 2009. Fast unifrac: Facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and phylochip data. ISME J. 4, 17-27.

Holmström, C. and Kjelleberg, S. 1999. Marine Pseudoalteromonas species are associated with higher organisms and produce biologically active extracellular agents. FEMS Microbiol. Ecol. 30, 285-293. crossref(new window)

Hong, P.Y., Hwang, C., Ling, F., Andersen, G.L., LeChevallier, M.W., and Liu, W.T. 2010. Pyrosequencing analysis of bacterial biofilm communities in water meters of a drinking water distribution system. Appl. Environ. Microbiol. 76, 5631-5635. crossref(new window)

Hwang, K., Oh, J., Kim, T.K., Kim, B.K., Yu, D.S., Hou, B.K., Caetano-Anolles, G., Hong, S.G., and Kim, K.M. 2013. Clustom: A novel method for clustering 16S rRNA next generation sequences by overlap minimization. PLoS One 8, e62623. crossref(new window)

Jones, P., Cottrell, M., Kirchman, D., and Dexter, S. 2007. Bacterial community structure of biofilms on artificial surfaces in an estuary. Microb. Ecol. 53, 153-162. crossref(new window)

Jukes, T.H. and Cantor, C.R. 1969. Evolution of protein molecules, pp. 21-32. In Munro, H.N. Mammalian Protein Metabolism, Academic Press, New York, NY, USA.

Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H., et al. 2012. Introducing eztaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716-721. crossref(new window)

Kwon, K.K., Lee, S.J., Park, J.H., Ahn, T.Y., and Lee, H.K. 2006. Psychroserpens mesophilus sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae isolated from a young biofilm. Int. J. Syst. Evol. Microbiol. 56, 1055-1058. crossref(new window)

Lane, D.J. 1991. 16S/23S rRNA sequencing, pp. 115-175. In Stackebrandt, E. and Goodfellow, M. (eds.), Nucleic Acid Techniques in Bacterial Systematics. John Wiley & Sons Press, New York, NY, USA.

Lau, S.C.K., Tsoi, M.M.Y., Li, X., Plakhotnikova, I., Dobretsov, S., Wu, M., Wong, P.K., Pawlik, J.R., and Qian, P.Y. 2006. Stenothermobacter spongiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a marine sponge in the Bahamas, and emended description of Nonlabens tegetincola. Int. J. Syst. Evol. Microbiol. 56, 181-185. crossref(new window)

Lee, O.O., Lau, S.C.K., Tsoi, M.M. Y., Li, X., Plakhotnikova, I., Dobretsov, S., Wu, M.C.S., Wong, P.K., and Qian, P.Y. 2006. Gillisia myxillae sp. nov., a novel member of the family Flavobacteriaceae, isolated from the marine sponge Myxilla incrustans. Int. J. Syst. Evol. Microbiol. 56, 1795-1799. crossref(new window)

Lee, J.W., Nam, J.H., Kim, Y.H., Lee, K.H., and Lee, D.H. 2008. Bacterial communities in the initial stage of marine biofilm formation on artificial surfaces. J. Microbiol. 46, 174-182. crossref(new window)

Lyautey, E., Jackson, C., Cayrou, J., Rols, J.L., and Garabetian, F. 2005. Bacterial community succession in natural river biofilm assemblages. Microb. Ecol. 50, 589-601. crossref(new window)

Maki, J.S., Little, B.J., Wagner, P., and Mitchell, R. 1990. Biofilm formation on metal surfaces in Antarctic waters. Biofouling 2, 27-38. crossref(new window)

Molin, S. and Tolker-Nielsen, T. 2003. Gene transfer occurs with enhanced efficiency in biofilms and induces enhanced stabilisation of the biofilm structure. Curr. Opin. Biotechnol. 14, 255-261. crossref(new window)

Moss, J.A., Nocker, A., Lepo, J.E., and Snyder, R.A. 2006. Stability and change in estuarine biofilm bacterial community diversity. Appl. Environ. Microbiol. 72, 5679-5688. crossref(new window)

Nedashkovskaya, O.I., Vancanneyt, M., Kim, S.B., and Zhukova, N.V. 2009. Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int. J. Syst. Evol. Microbiol. 59, 1465-1468. crossref(new window)

Nichols, C.M., Bowman, J.P., and Guezennec, J. 2005. Olleya marilimosa gen. nov., sp. nov., an exopolysaccharide-producing marine bacterium from the family Flavobacteriaceae, isolated from the southern ocean. Int. J. Syst. Evol. Microbiol. 55, 1557-1561. crossref(new window)

Oh, J., Kim, B.K., Cho, W.S., Hong, S.G., and Kim, K.M. 2012. Pyrotrimmer: A software with gui for pre-processing 454 amplicon sequences. J. Microbiol. 50, 766-769. crossref(new window)

Pohlon, E., Marxsen, J., and Küsel, K. 2010. Pioneering bacterial and algal communities and potential extracellular enzyme activities of stream biofilms. FEMS Microbiol. Ecol. 71, 364-373. crossref(new window)

Sakshaug, E. and Slagstad, D.A.G. 1991. Light and productivity of phytoplankton in polar marine ecosystems: a physiological view. Polar Res. 10, 69-86. crossref(new window)

Salta, M., Wharton, J.A., Blache, Y., Stokes, K.R., and Briand, J.F. 2013. Marine biofilms on artificial surfaces: Structure and dynamics. Environ. Microbiol. 15, 2879-2893.

Schloss, P.D., Westcott, S.L., Ryabin, T., Hall, J.R., Hartmann, M., Hollister, E.B., Lesniewski, R.A., Oakley, B.B., Parks, D.H., Robinson, C.J., et al. 2009. Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol. 75, 7537-7541. crossref(new window)

Sekar, R., Nair, K.V.K., Rao, V.N.R., and Venugopalan, V.P. 2002. Nutrient dynamics and successional changes in a lentic freshwater biofilm. Freshwater Biol. 47, 1893-1907. crossref(new window)

Stewart, C.N. Jr and Via, L.E. 1993. A rapid ctab DNA isolation technique useful for rapd fingerprinting and other PCR applications. Biotechniques 14, 748-758.

Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725-2729. crossref(new window)

Vandecandelaere, I., Nercessian, O., Faimali, M., Segaert, E., Mollica, A., Achouak, W., Vos, P.D., and Vandamme, P. 2010. Bacterial diversity of the cultivable fraction of a marine electroactive biofilm. Bioelectrochemistry 78, 62-66. crossref(new window)

Wang J., Liu, M., Xiao, H., Wu, W., Xie, M., Sun, M., Zhu, C., and Li, P. 2013. Bacterial community structure in cooling water and biofilm in an industrial recirculating cooling water system. J. Bacteriol. 182, 2675-2679.

Watnick, P. and Kolter, R. 2000. Biofilm, city of microbes. J. Bacteriol. 182, 2675-2679. crossref(new window)

Webster, N., Battershill, C., and Negri, A. 2006. Recruitment of antarctic marine eukaryotes onto artificial surfaces. Polar Biol. 30, 1-10. crossref(new window)

Webster, N.S. and Negri, A.P. 2006. Site-specific variation in antarctic marine biofilms established on artificial surfaces. Environ. Microbiol. 8, 1177-1190. crossref(new window)

Widmer, F., Hartmann, M., Frey, B., and Kölliker, R. 2006. A novel strategy to extract specific phylogenetic sequence information from community T-RFLP. J. Microbiol. Methods 66, 512-520. crossref(new window)