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Phylogenetic diversity and UV resistance analysis of radiation-resistant bacteria isolated from the water in Han River
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 Title & Authors
Phylogenetic diversity and UV resistance analysis of radiation-resistant bacteria isolated from the water in Han River
Lee, Jae-Jin; Joo, Eun Sun; Lee, Do Hee; Jung, Hee-Young; Kim, Myung Kyum;
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 Abstract
The aim of this study was to investigate the UV-resistance of radiation-resistant bacteria isolated from the water of Han River, South Korea. The water sample was irradiated with 3 kGy gamma radiation prior to isolation. Radiation-resistant bacterial strains were isolated by standard serial dilution method on R2A and 1/10 diluted R2A agar. The resulting purely isolated 60 cultures of bacteria were analysed for UV resistance and used in further studies. Based on the comparative analyses of 16S rRNA gene sequences, the bacterial isolates were divided into 3 phyla (4 genera): the phylum Deinococcus-Thermus (the genus Deinococcus) was 61.7%, Bacteroidetes (Hymenobacter and Spirosoma) was 23.4%, and Firmicutes (Exiguobacterium) was 15%. The results suggested that twenty-nine isolates are candidates new species belonging to Deinococcus, Hymenobacter, and Spirosoma, or other new genera. Nine bacterial strains were selected among the novel candidates and the UV-resistance analysis was conducted. All the candidate bacterial strains showed high UV resistance, similar to that of D. radiodurans R1.
 Keywords
16S rDNA;Han River;phylogenetic tree;radiation-resistant bacteria;UV resistance analysis;water;
 Language
Korean
 Cited by
 References
1.
Asker, D., Awad, T.S., Beppu, T., and Ueda, K. 2009. Deinococcus aquiradiocola sp. nov., isolated from a radioactive site in Japan. Int. J. Syst. Evol. Microbiol. 59, 144-149. crossref(new window)

2.
Asker, D., Awad, T.S., McLandsborough, L., Beppu, T., and Ueda, K. 2011. Deinococcus depolymerans sp. nov., a gamma and UV-radiation-resistant bacterium, isolated from a naturally radioactive site. Int. J. Syst. Evol. Microbiol. 61, 1448-1453. crossref(new window)

3.
Bae, J.Y., Kim, K.Y., Kim, J.H., Lee, K., Cho, J.C., and Cha, C.J. 2010. Paenibacillus aestuarii sp. nov., isolated from an estuarine wetland. Int. J. Syst. Evol. Microbiol. 60, 644-647. crossref(new window)

4.
Baik, K.S., Kim, M.S., Park, S.C., Lee, D.W., Lee, S.D., Ka, J.O., Choi, S.K., and Seong, C.N. 2007. Spirosoma rigui sp. nov., isolated from fresh water. Int. J. Syst. Evol. Microbiol. 57, 2870-2873. crossref(new window)

5.
Brooks, B.W. and Murray, R.G.E. 1981. Nomenclature for "Micrococcus radiodurans" and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int. J. Syst. Bacteriol. 19, 353-360.

6.
Cha, S., Srinivasan, S., Seo, T., and Kim, M.K. 2014. Deinococcus radiotolerans sp. nov., a gamma-radiation resistant bacterium isolated from gamma ray-irradiated soil. Antonie van Leeuwenhoek 105, 229-235. crossref(new window)

7.
Choi, M.J., Bae, J.Y., Kim, K.Y., Kang, H., and Cha, C.J. 2010. Brevibacillus fluminis sp. nov., isolated from sediment of estuarine wetland. Int. J. Syst. Evol. Microbiol. 60, 1595-1599. crossref(new window)

8.
Cox, M.M. and Battista, J.R. 2005. Deinococcus radiodurans - the consummate survivor. Nature Rev. Microbiol. 3, 882-892. crossref(new window)

9.
de Groot, A., Chapon, V., Servant, P., Christen, R., Saux, M.F., Sommer, S., and Heulin, T. 2005. Deinococcus deserti sp. nov., a gamma radiation-tolerant bacterium isolated from the Sahara Desert. Int. J. Syst. Evol. Microbiol. 55, 2441-2446. crossref(new window)

10.
Gabani, P. and Singh, O.V. 2013. Radiation-resistant extremophiles and their potential in biotechnology and therapeutics. Appl. Microbiol. Biotechnol. 97, 993-1004. crossref(new window)

11.
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41, 95-98.

12.
Hirsch, P., Gallikowski, C.A., Siebert, J., Peissl, K., Kroppenstedt, R., Schumann, P., Stackebrandt, E., and Anderson, R. 2004. Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draughttolerating, UV-resistant bacteria from continental Antarctica. Syst. Appl. Microbiol. 27, 636-645. crossref(new window)

13.
Hirsch, P., Ludwig, W., Hethke, C., Sittig, M., Hoffmann, B., and Gallikowski, C.A. 1998. Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctic soils and sandstone: bacteria of the cytophaga/flavobacterium/bacteroides line of phylogenetic descent. Syst. Appl. Microbiol. 21, 374-383. crossref(new window)

14.
Im, W.T., Jung, H.M., Ten, L.N., Kim, M.K., Bora, N., Goodfellow, M., Lim, S., Jung, J., and Lee, S.T. 2008. Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 58, 2348-2353. crossref(new window)

15.
Kampfer, P., Lodders, N., Huber, B., Falsen, E., and Busse, H.J. 2008. Deinococcus aquatilis sp. nov., isolated from water. Int. J. Syst. Evol. Microbiol. 58, 2803-2806. crossref(new window)

16.
Klassen, J.L. and Foght, J.M. 2011. Characterization of Hymenobacter isolates from Victoria Upper Glacier, Antarctica reveals five new species and substantial non-vertical evolution within this genus. Extremophiles 15, 45-57. crossref(new window)

17.
Kwon, J.Y. and Seo, Y.R. 2010. Genome-wide profiling induced by ionizing radiation (IR) in non-small cell lung cancer (NSCLC) grown as three-dimensional spheroid. Mol. Cell. Toxicol. 6, 229-237. crossref(new window)

18.
Lai, W.A., Kampfer, P., Arun, A.B., Shen, F.T., Huber, B., Rekha, P.D., and Young, C.C. 2006. Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. Int. J. Syst. Evol. Microbiol. 56, 787-791. crossref(new window)

19.
Lee, J.J., Kang, M.S., Joo, E.S., Kim, M.K., and Srinivasan, S. 2015. Spirosoma montaniterrae sp. nov., an ultraviolet and gamma radiation-resistant bacterium isolated from mountain soil. J. Microbiol. 53, 429-434. crossref(new window)

20.
Lee, J.J., Srinivasan, S., Lim, S., Joe, M., Im, S., Bae, S.I., Park, K.R., Han, J.H., Park, S.H., Joo, B.M., et al. 2014. Spirosoma radiotolerans sp. nov., a gamma-radiation-resistant bacterium isolated from gamma ray-irradiated soil. Cur. Microbiol. 69, 286-291. crossref(new window)

21.
Mattimore, V. and Battista, J.R. 1996. Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J. Bacteriol. 178, 633-637. crossref(new window)

22.
Park, S.J., Choi, J.H., and Cha, C.J. 2013. Chryseobacterium rigui sp. nov., isolated from an estuarine wetland. Int. J. Syst. Evol. Microbiol. 63, 1062-1067. crossref(new window)

23.
Rainey, F.A., Ray, K., Ferreira, M., Gatz, B.Z., Nobre, M.F., Bagaley, D., Rash, B.A., Park, M.J., Earl, A.M., Shank, N.C., et al. 2005. Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Spirosoma obtained from a single soil sample. Appl. Environ. Microbiol. 71, 5225-5235. crossref(new window)

24.
Rodrigues, D.F., Goris, J., Vishnivetskaya, T., Gilichinsky, D., Thomashow, M.F., and Tiedje, J.M. 2006. Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov. Extremophiles 10, 285-294 crossref(new window)

25.
Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.

26.
Servinsky, M.D. and Julin, D.A. 2007. Effect of a recD mutation on DNA damage resistance and transformation in Deinococcus radiodurans. J. Bacteriol. 189, 5101-5107. crossref(new window)

27.
Shashidhar, R. and Bandekar, J.R. 2009. Deinococcus piscis sp. nov., a radiation-resistant bacterium isolated from a marine fish. Int. J. Syst. Evol. Microbiol. 59, 2714-2717. crossref(new window)

28.
Suresh, K., Reddy, G.S.N., Sengupta, S., and Shivaji, S. 2004. Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. Int. J. Syst. Evol. Microbiol. 54, 457-461. crossref(new window)

29.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA5: molecular evolutionary genetics 298 analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731-2739. crossref(new window)

30.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 4876-4882.

31.
Wang, W., Mao, J., Zhang, Z., Tang, Q., Xie, Y., Zhu, J., Zhang, L., Liu, Z., Shi, Y., and Goodfellow, M. 2010. Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil. Int. J. Syst. Evol. Microbiol. 60, 2006-2010. crossref(new window)

32.
White, O., Eisen, J.A., Heidelberg, J.F., Hickey, E.K., Peterson, J.D., Dodson, R.J., Haft, D.H., Gwinn, M.L., Nelson, W.C., Richardson, D.L., et al. 1999. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science 286, 1571-1577. crossref(new window)

33.
Woo, P.C., Lau, S.K., Teng, J.L., and Yuen, K.Y. 2008. Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin. Microbiol. Infect. 14, 908-909. crossref(new window)

34.
Yoo, S.H., Weon, H.Y., Kim, S.J., Kim, Y.S., Kim, B.Y., and Kwon, S.W. 2010. Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples. Int. J. Syst. Evol. Microbiol. 60, 1191-1195. crossref(new window)

35.
Yu, L.Z., Luo, X.S., Liu, M., and Huang, Q. 2015. Diversity of ionizing radiation-resistant bacteria obtained from the Taklimakan Desert. J. Basic Microbiol. 55, 135-140. crossref(new window)

36.
Yuan, M., Zhang, W., Dai, S., Wu, J., Wang, Y., Tao, T., Chen, M., and Lin, M. 2009. Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium. Int. J. Syst. Evol. Microbiol. 59, 1513-1517. crossref(new window)

37.
Zhang, Y.Q., Sun, C.H., Li, W.J., Yu, L.Y., Zhou, J.Q., Xu, L.H., and Jiang, C.L. 2007. Deinococcus yunweiensis sp. nov., a gamma- and UV radiation-resistant bacterium from China. Int. J. Syst. Evol. Microbiol. 57, 370-375. crossref(new window)