Advanced SearchSearch Tips
Inhibition of cell wall synthesis in Cryptococcus neoformans and decrease of skin allergy induced with Alternaria alternata in mouse model by a chitinase from an inky cap
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Inhibition of cell wall synthesis in Cryptococcus neoformans and decrease of skin allergy induced with Alternaria alternata in mouse model by a chitinase from an inky cap
Kang, Yuri; Choi, Hyoung T.;
  PDF(new window)
The growth of two different fungal species, human pathogenic Cryptococcus neoformans and plant pathogenic Alternaria alternata were inhibited by a chitinase (Chi2) expressed in the autolysing tissue of Coprinellus congregatus. The cell wall thickness was reduced (up to 32%) in C. neoformans compared with that of normal cell, and polysaccharide fibers located outside of the cell wall were also severely removed. The hyphal growth of A. alternata on agar plate was stopped by the enzyme. The allergic inflammation induced by A. alternata was reduced by the enzyme reaction when compared with untreated control in a mouse model.
Alternaria alternata;Cryptococcus neoformans;allergy;chitinase;
 Cited by
Amian, A.A., Papenbrock, J., Jacobsen, H.J., and Hassan, F. 2011. Enhancing transgenic pea (Pisum sativum L.) resistance against fungal diseases through stacking of two antifungal genes (chitinase and glucanase). GM Crops 2, 104-109. crossref(new window)

Arora, S., Hernandez, Y., Erb-Downward, J.R., McDonald, R.A., Toews, G.B., and Huffnagle, G.B. 2005. Role of IFN-${\gamma}$ in regulating T2 immunity and the development of alternatively activated macrophages during allergic bronchopulmonary mycosis. J. Immunol. 174, 6346-6356. crossref(new window)

Baker, L.G., Specht, C.A., and Lodge, J.K. 2011. Cell wall chitosan is necessary for virulence in the opportunistic pathogen Cryptococcus neoformans. Eukaryot. Cell 10, 1364-1368.

Brito, F.F., Alonso, A.M., Carnes, J., Martin-Martin, R., Fernandez-Caldas, E., Galindo, P.A., Alfaya, T., and Amo-Salas, M. 2012. Correlation between Alt a1 levels and clinical symptoms in Alternaria alternata-Monosensitized patients. J. Investig. Allergol. Clin. Immunol. 22, 154-159.

Choi, H.T. and Cho, C.W. 2005. Ultrastructural studies on the autolysis of Coprinellus con-gregatus. Korean J. Microbiol. 41, 312-315.

Distefano, G., Malfa, S.L., Vitale, A., Lorito, M., Deng, Z., and Gentile, A. 2008. Defence-related gene expression in transgenic lemon plants producing an antimicrobial Trichoderma harzianum endochitinase during fungal infection. Transgenic Res. 17, 873-879. crossref(new window)

Kang, K. and Choi, H.T. 2012. Growth inhibition of plant pathogenic fungi by a chitinase of Coprinellus congregates. Korean J. Microbiol. 48, 325-327. crossref(new window)

Kang, Y., Kim, H., and Choi, H.T. 2013. Biochemical characterization of chitinase 2 expressed during the autolytic phase of the inky cap, Coprinellus congregatus. J. Microbiol. 51, 189-193. crossref(new window)

Lee, K.K., MacCallum, D.M., Jacobsen, M.D., Walker, L.A., Odds, F.C., Gow, N.A.R., and Munro, C.A. 2012. Elevated cell wall chitin in Candida albicans confers echinocandin resistance in vivo. Antimicrob. Agents Chemother. 56, 208-217. crossref(new window)

Lopez-Matas, M.A., Aslava, A.P., and Diaz-Minguez, J.M. 2000. Mcchs1, a member of a chitin synthase gene family in Mucor circinelloides, is differentially expressed during dimorphism. Microbiology 40, 169-175.

Lyons, T.W., Wakefield, D.B., and Cloutier, M.M. 2011. Mold and Alternaria skin test reactivity and asthma in children in Connecticut. Ann. Allergy Asthma Immunol. 106, 301-307. crossref(new window)

Sharpe, R.A., Bearman, N., Thornton, C.R., Husk, K., and Osborne, N.J. 2015a. Indoor fungal diversity and asthma: a meta-analysis and systemic review of risk. J. Allergy Clin. Immunol. 135, 110-122. crossref(new window)

Sharpe, R.A., Thornton, C.R., Tyrrell, J., Nikolaou, V., and Osborne, N.J. 2015b. Variable risk of atopic disease due to indoor fungal exposure in NHANES 2005-2006. Clin. Exp. Allergy 45, 1478-1482. crossref(new window)

Yoo, Y. and Choi, H.T. 2014. Antifungal chitinase against human pathogenic yeasts from Coprinellus congregates. Korean J. Microbiol. 52, 441-443.

Zhao, J., Li, S., Jiang, T., Liu, Z., Zhang, W., Jian, G., and Qi, F. 2012. Chilling stress-the key predisposing factor for causing Alternaria alternata infection and leading to cotton (Gossypium hirsutum L.) leaf senescence. PLoS One 7, 1-11.