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Reactive oxygen species-dependent down-regulation of ubiquitin C-terminal hydrolase in Schizosaccharomyces pombe
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 Title & Authors
Reactive oxygen species-dependent down-regulation of ubiquitin C-terminal hydrolase in Schizosaccharomyces pombe
Jo, Hannah; Lim, Hye-Won; Kwon, Hee-Souk; Lim, Chang-Jin; Park, Kwang Hark; Jin, Chang Duck; Kim, Kyunghoon;
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 Abstract
The Schizosaccharomyces pombe gene, belonging to the PPPDE superfamily of deubiquitinating enzyme (DUB) genes, was previously shown to encode a protein with ubiquitin C-terminal hydrolase (UCH) activity and to participate in the response against oxidative and nitrosative stresses. This work focused on the reactive oxygen species (ROS)-dependent regulation of the S. pombe gene. UCH activities, encoded by the gene, were attenuated in the S. pombe cells exposed to , superoxide radical-generating menadione (MD), and nitric oxide (NO)-generating sodium nitroprusside (SNP). Reduced glutathione (GSH) and its precursor N-acetylcysteine (NAC) were able to significantly enhance the UCH activities in the absence or presence of . However, the influences of both GSH and NAC on the ROS levels in the absence or presence of were opposite to their effects on the UCH activities under the same conditions. The UCH activities in the Sdu1-overexpressing S. pombe cells were also diminished under exposure to , MD and SNP, but still remained to be higher than those in the vector control cells. In brief, it is proposed that the S. pombe gene is regulated by ROS in a negative manner, the meaning of which largely remains elusive.
 Keywords
;Schizosaccharomyces pombe;deubiquitinating enzyme;reactive oxygen species;ubiquitin C-terminal hydrolase;
 Language
English
 Cited by
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