Chitosan, PVA, 그리고 Chitosan/PVA 피막의 특성과 사과 겹무늬썩음병균 Botryosphaeria dothidea의 포자분산 억제작용

  • 이승지 (경북대학교 자연과학대학 유전공학과) ;
  • 박동찬 (경북대학교 자연과학대학 유전공학과) ;
  • 김이부 (경북대학교 농과대학 농생물학과) ;
  • 엄재열 (경북대학교 농과대학 농생물학과) ;
  • 이용현 (경북대학교 자연과학대학 유전공학과)
  • Published : 1996.10.01

Abstract

The applicability of chitosan, a biodegradable natural polymer, as the coating material to prevent the dispersal of the spores of the apple white rot agent Botryosphaeria dothidea, was investigated. The physical properties of mixed chitosan/polyvinyl alcohol(PVA) film showed the increased physical properties for film formation, such as tensil strength, elongation, and viscosity, compared to either chitosan or PVA film. The FT-IR spectra of chitosan/PVA film indicated that the film was formed by simple blending not by any new synthetic bond. The chitosan and chitosan/PVA film showed effective antifungal activity on B. dothidea. The formed films were well decomposed by ASTM strains used for biodegradability test, on the other hand, the PVA film could not be decomposed by above standard strains. The field test at apple orchard showed that the dispersal of B. dothidea spores could be effectively reduced by coated film, especially by chitosan/PVA film. The spore dispersal was reduced upto 97.0% by 1.0% chitosan/5.0% PVA film during 4 months.

Keywords

References

  1. Phytopathology v.71 Production and dispersal of ascospores and conidia by Physalospora obtusa and Botryosphaeria dothidea in apple orchards. Sutton, T.B.
  2. 경북대학교 농업과학기술연구소 미국의 식물검역 기준에 적합한 사과 병해 방제체계 수립. 엄재열;이준탁
  3. 경북대학교 학위논문집. 사과 겹무늬썩음병균의 포자형성 및 분산저지법 개발. 김대희
  4. 산업미생물학회지 v.24 Chitosan이 사과 겹무늬썩음병균 Botryosphaeria dothidea 생육에 미치는 영향. 이승지;엄재열;이용현
  5. 경북대학교학위논문집. Polymer coating에 의한 사과 겹무늬썩음병균 포자분산 저지법의 개발. 김이부
  6. 주류공업 v.14 Chitin/chitosan 유도체의 구조, 분해효소, 이용 및 주류산업에의 활용. 이용현;정의준.
  7. Chitin, Chitosan, and Related Enzymes Chitosan, a natural regulator in plant-fungal pathogen interactions, increases for crop yields. Lee, A.H;F.Brian;C.R.Robert;P.Z.John(ed.)
  8. Annual Book of ASTM Standards. v.06;01 Standard practice for determining resistance of synthetic polymeric materials to fungi. ASTM G21-90. ASTM.
  9. 한국공업규격 KS M 3006.(공업진흥청고시 제83-313호). 플라스틱의 인장성 측정 방법. 한국공업표준협회.
  10. Kor. Jour. Microbiol. v.22 An improved method for the measurement of fungal degradability of synthetic polymer. Rhee, Y.H;J.A.Lee;P.J.Maeng;C.L.Jun.
  11. Paints and Their Applications. v.2 Surface coating. Oil and Colour Chemists' Association.
  12. 한국생물공학회지 v.8 Chitin과 cellulose 복합체로부터 새로운 생분해성 chitulose film의 개발. 류영석;이충우;홍범식;윤정원.
  13. Appl. Environ. Microbiol. v.49 Localization of polyvinyl alcohol oxidase produced by a bacterical symbiont, Pseudomonas sp. strain VM15C. Masayuki, S;N.Yuji;K.Nobuo;S.Chikahiro
  14. Agric. Biol. Chem. v.49 A new enzyme, β-diketone hydrolase: a component of a poly(vinyl alcohol)-degrading enzyme preparation. Kiyofumi,S;H.Nobutake;W.Yasuto.