Advanced SearchSearch Tips
Comparative Study of the Efficiency of GC with Large Volume Injector and SPE Clean-up Process Applied in QuEChERS Method
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Comparative Study of the Efficiency of GC with Large Volume Injector and SPE Clean-up Process Applied in QuEChERS Method
Park, Young Jun; Hong, Su Myeong; Kim, Taek Kyum; Kwon, Hye Young; Hur, Jang Hyun;
  PDF(new window)
This study was conducted to compare STQ method, multi-residue method in Korean food code and QuEChERS method for validated selected and accuracy, reproducibility and efficiency. A total of 45 selected and targeted pesticides were the analyzed by GC and 5 of them were crops (apple, potato, green pepper, rice, soy bean). values were calculated in the standard calibration curve was over 0.990. Recovery tests were performed by three replications in two levels and the relative standard deviation of the repeated experiments was less than 30%. The average percentage of recoveries in the multi-residue method in Korean food code was 89.13%, QuEChERS method was 92.45% and STQ method was 85.28%. In addition, matrix effects in multi-residue method in Korean food code was 24.61%, QuEChERS method was 23.98% and STQ method showed 11.24%. The STQ method is easy and showed high clean-up effect in extracting the sample solution than the QuEChERS method and clean-up with C18, PLS, PSA cartridge columns. A large volume of the sample was injected in order to compensable for the problem, that occurred due to high detection limit in the analyser. When the STQ method was applied using a large volume injector, the standard calibration curve showed a higher linearity , and method detection limit was 0.01 mg/kg. It showed an average recovery of 91.84% and the relative standard deviations of three replications repeated in two level process was less than 30% and had an average matrix effect of 17.90%.
Multi-residue pesticide;QuEChERS;STQ;LVI;PTV;Matrix effect;
 Cited by
Anastassiades, Michelangelo, et al. (2003) Fast and easy multiresidue method employing acetonitrile extraction/ partitioning and "dispersive solid-phase extraction"for the determination of pesticide residues in produce. Journal of AOAC international 86.2:412-431.

Chamkasem, Narong, et al. (2013) Analysis of 136 Pesticides in Avocado Using a Modified QuEChERS Method with LC-MS/MS and GC-MS/MS. Journal of agricultural and food chemistry 61.10:2315-2329. crossref(new window)

Gonzlez-Castro, Beceiro-Gonzles EMJ. (2008) Organochlorine pesticide determination in horticultural samples. Pesticide Research Trends. p.13-48.

Hajlov, J., et al. (1998) Matrix-induced effects: a critical point in the gas chromatographic analysis of pesticide residues. Journal of Chromatography A 800.2:283-295. crossref(new window)

Hill, A. R. and S. L. Reynolds (1999) Guidelines for in-house validation of analytical methods for pesticide residues in food and animal feeds. The Analyst 124.6:953-958. crossref(new window)

Hoh, Eunha, and Katerina Mastovska. (2008) Large volume injection techniques in capillary gas chromatography. Journal of Chromatography A 1186.1:2-15. crossref(new window)

Ju-young Lee (2012) Development of Multi-Residue Analysis Method of Residue Pesticides in Fruits by QuEChERS. Kyung-pook National University.

Koesukwiwat, Urairat, Kunaporn Sanguankaew, and Natchanun Leepipatpiboon (2008) Rapid determination of phenoxy acid residues in rice by modified QuEChERS extraction and liquid chromatographytandem mass spectrometry. Analytica chimica acta 626.1:10-20. crossref(new window)

Matuszewski, B. K. (2006) Standard line slopes as a measure of a relative matrix effect in quantitative HPLCMS bioanalysis. Journal of Chromatography B 830.2:293-300. crossref(new window)

Min-jung Kim, et al. (2005) Application of the Pesticide Multiresidue Analysis Method for Potatoes and Carrots 37.2:304-307.

National Institute of Food and Drug Safety Evaluation (2014) Korean Food Standards Codex-Pesticide Analytical Manual.

Niessen, W. M. A., P. Manini and R. Andreoli (2006) Matrix effects in quantitative pesticide analysis using liquid chromatography mass spectrometry. Mass Spectrometry Reviews 25.6:881-899. crossref(new window)

Schellin, Manuela, Barbara Hauser, and Peter Popp (2004) Determination of organophosphorus pesticides using membrane-assisted solvent extraction combined with large volume injectiongas chromatographymass spectrometric detection. Journal of Chromatography A 1040.2:251-258. crossref(new window)

Schenck, Frank J., and Steven J. Lehotay (2000) Does further clean-up reduce the matrix enhancement effect in gas chromatographic analysis of pesticide residues in food?. Journal of chromatography A 868.1:51-61. crossref(new window)

Teske, J., J. Efer, and W. Engewald. (1997) Large-volume PTV injection: new results on direct injection of water samples in GC analysis. Chromatographia 46.11-12: 580-586. crossref(new window)

Wilkowska, Angelika, and Marek Biziuk (2011) Determination of pesticide residues in food matrices using the QuEChERS methodology. Food Chemistry 125.3: 803-812. crossref(new window)

Young-Min Hong, Ryoichi sasano, Yutaka Nakasishi (2005) Rapid Sample Preparation and Multiple Pesticides Residue Analysis in Food by GC/MS using Stomach Shaped Large Volume Injector. The Korea Society for Environmental Analysis. 8.3: 154-161.