Advanced SearchSearch Tips
Improvement of Seed Germination in Rosa rugosa
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Improvement of Seed Germination in Rosa rugosa
Lee, Ji-Yeon; Lee, Ja-Hyun; Ki, Gwang-Yeon; Kim, Seung-Tae; Han, Tae-Ho;
  PDF(new window)
Rose seed shows low germination percentages (about 20%) because it has a high amount of substances that inhibit germination in the pericarp. We investigated the effect of orientation of achene, the day after pollination (DAP), and cold storage with or without half-cut in achene for the germination percentage in R. rugosa. Germination percentages of intact or half-cut achenes were investigated in a 16-hour photoperiod at room on basal MS medium for two weeks. In germination percentage, maximum 100% was measured within one week when half-cut achenes were cultured on an orientation that the embryos facing to the light. Half-cut achenes at 90 DAP were germinated 100% regardless of cold storage. Various LED lights (red, blue, yellow, green, and white) were illuminated over the half-cut achenes to gain the effect of light color. Germination percentage of R. rugosa seeds under blue LED reached the greatest with 90% within one week of culture and these seedlings were the best with a steady growth rate. It is concluded that half-cut achenes would be an effective method to improve seed germination in R. rugosa without stratification or scarification. This system could be applied to breeding studies in rose cultivars.
achene;half-cut seed;in vitro culture;light-emitting diode (LED);orientation;rose;
 Cited by
해당화 열매 추출 분말의 항산화 활성과 고지방 식이로 유도된 Mice의 혈청지질 수준에 미치는 영향,최경순;김용환;이기원;신경옥;

한국식품영양학회지, 2015. vol.28. 2, pp.320-327 crossref(new window)
Arunachalam, V. and U.S. Kaicker. 1994. In vitro germination a potential commercial method for roses. p. 410-412. In: J. Prakash and K.R. Bhandry (eds.). Floriculture, technology, trades and trands. Oxford and IBH press, New Delhi.

Cho, J.Y., D.M. Son, J.M Kim, B.S. Seo, and S.Y. Yang. 2008. Effects of various LEDs on the seed germination, growth and physiological activities of rape (Brassica napus) sprout vegetable. Kor. J. Plant. Res. 2:304-309.

Choi, Y.H., M.J. Kim, H.S. Lee, C. Hu, and S.S. Kwak. 1997. Antioxidants in leaves of Rosa rugosa. Kor. J. Phamacogn. 26:179-184.

Gi, G.Y., K.J. Choi, T.S. Na, M.S. Cho, Y.S. Lee, J.K. Kim, J.S. Lee, and T.H. Han. 2006. Breeding of a new rose cultivar 'Hanmaeum' with red-white color flower and resistance to the powdery mildew. Kor. J. Hort. Sci. Technol. 24:388-391.

Gudin, S., L. Arene, A. Chavagnant, and C. Bulard. 1990. Influence of endocarp thickness on rose achene germination: Genetic and environmental factors. HortScience 25:786-788.

Jackson, G.A.D and J.B. Blundell. 1963. Germination in Rosa. J. Hort. Sci. 38:310-320.

Jackson, G.A.D. 1968. Hormonal control of fruit development, seed dormancy and germination with particular reference to Rosa. Soc. Chem. Ind. Mongr. 31:127-156.

Jakobsone, G., D. Rieksta, and A. Rihtere. 2006. Breeding of Rosa rugosa Thunb. using different tissue culture methods. Acta Hort. 725:211-216.

Jin, B., H.R. Dong, X.H. Yang, and D.W. Zhu. 1995. Shortening hybridization breeding cycle of rose - A study on mechanism controlling achene dormancy. Acta Hort. 404:40-47.

Kang, S.C., J.D. Lim, J.C. Lee, H.J. Park, N.S. Kang, and E.H. Sohn. 2010. Effects of fructus and semen from Rosa rugosa on osteoimmune cells. Kor. J. Plant Res. 23:157-164.

Kazaz, S., S. Erbas, and H. Baydar. 2010. Breaking seed dormancy in oil rose (Rosa damascene Mill.) by microbial inoculation. Afr. J. Biotech. 39:6503-6508.

Lammerts, W.E. 1946. Use of embryo culture in rose breeding. Plants Gardens 2:111.

Lee, Y.S., J.W. Chung, K.B. An, and J.W. Lim. 2006. A new rose (Rosa hybrida) cultivar 'Party Queen' with bright pink color. Kor. J. Breed. 38:271-272.

Ministry for Food, Agriculture, Forestry and Fisheries (MIFAFF). 2007. Annual report of floriculture. MIFAFF, Gwachen, Korea p. 15-21.

Murashige, T. and F. Skoog. 1962. Revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15:473-497.

Nhut, D.T., T. Takamura, H. Watanabe, K. Okamoto, and M. Tanaka. 2003. Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tiss. Organ Cult. 73:43-52.

Okamoto, K., T. Yanagi, S. Takita, M. Tanaka, T. Higuchi, Y.Ushida, and H. Watanebe. 1996. Development of plant growth apparatus using blue and red LED as artificial light source. Acta Hort. 440:111-116.

Park, J.C., S.C. Kim, M.R. Choi, S.H. Song, E.J. Yoo, S.H. Kim, H. Miyashiro, and M. Hattori. 2005. Anti-HIV proteaseactivity from Rosafamily plant extracts and rosamultin from Rosa rugosa. J. Med. Food 8:107-109.

Tillberg, E. 1983. Levels of endogenous abscisic acid in achenes of Rosa rugosa during dormancy release and germination. Physiol. Planta 58:243-248.

Yambe, Y. and K. Takeno. 1992. Improvement of rose achene germination by treatment with macerating enzymes. HortScience 27:1018-1020.

Younis, A., A. Riaz, R. Ahmed, and A. Raza. 2007. Effect of hot water, sulphuric acid and nitric acid on the germination of rose seeds. Acta Hort. 755:104-108.

Zhou, Z.Q., W.K. Bao, and N. Wu. 2009. Dormancy and germination in Rosa multibracteata Hemsl. & E. H. Wilson. Sci. Hort. 19:434-441.