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Improvement of Seed Germination in Rosa rugosa
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 Title & Authors
Improvement of Seed Germination in Rosa rugosa
Lee, Ji-Yeon ; Lee, Ja-Hyun ; Ki, Gwang-Yeon ; Kim, Seung-Tae ; Han, Tae-Ho ;
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 Abstract
Rose seed shows low germination percentages (about 20%) because it has a high amount of substances that inhibit germination in the pericarp. We investigated the effect of orientation of achene, the day after pollination (DAP), and cold storage with or without half-cut in achene for the germination percentage in R. rugosa. Germination percentages of intact or half-cut achenes were investigated in a 16-hour photoperiod at room on basal MS medium for two weeks. In germination percentage, maximum 100% was measured within one week when half-cut achenes were cultured on an orientation that the embryos facing to the light. Half-cut achenes at 90 DAP were germinated 100% regardless of cold storage. Various LED lights (red, blue, yellow, green, and white) were illuminated over the half-cut achenes to gain the effect of light color. Germination percentage of R. rugosa seeds under blue LED reached the greatest with 90% within one week of culture and these seedlings were the best with a steady growth rate. It is concluded that half-cut achenes would be an effective method to improve seed germination in R. rugosa without stratification or scarification. This system could be applied to breeding studies in rose cultivars.
 Keywords
achene . half-cut seed . in vitro culture . light-emitting diode (LED) . orientation . rose
 Language
Korean
 Cited by
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