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Foliar Application of Growth Bioregulators Influences Floral Traits, Cormassociated Traits and Chemical Constituents in Gladiolus grandiflorus L.
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 Title & Authors
Foliar Application of Growth Bioregulators Influences Floral Traits, Cormassociated Traits and Chemical Constituents in Gladiolus grandiflorus L.
Sajjad, Yasar; Jaskani, Muhammad Jafar; Qasim, Muhammad; Akhtar, Gulzar; Mehmood, Asim;
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Gladiolus is one of the most popular cut flowers in the world floral market and is highly attractive to consumers. The production of poor-quality gladiolus spikes is one of the most important hurdles to obtaining high prices for this crop. The main objectives of this study were to improve the quantitative characteristics of gladiolus spikes, to increase propagule production and to enhance chemical constituent levels in leaves to help improve plant growth. We performed foliar application of bioregulators, including polyamines (putrescine and spermine) and vitamins (ascorbic acid and thiamine), at different concentrations (0, 0.1, 0.4, 0.7 and 1 mM) to gladiolus plants at the three leaf, five leaf and slipping stages. Application of 1 mM putrescine was the most effective treatment, with prominent effects on economically important spike traits including spike length (55.53 cm), spike diameter (7.53 mm), florets per spike (13.99), first floret diameter (9.66 cm) and first floret weight (4.90 g), followed by spermine treatment. The 1 mM putrescine treatment also had positive effects on corm-associated traits including corm diameter and corm weight, with values of 4.57 cm and 26.16 g compared to 3.47 cm and 17.16 g in control plants, respectively. The contents of chemical constituents in leaves, including total chlorophylls (), total carotenoids (), total soluble sugars () and phenolics () increased in response to foliar application of 1 mM putrescine compared to the control. Ascorbic acid and thiamine also had positive effects on various plant morphological traits and chemical constituents in leaves compared to control plants, but the growth-promoting effects of polyamines were more pronounced. Hence, applying polyamines to gladiolus leaves will help improve spike production and increasing propagule (cormel) yields.
bulbous plant;growth enhancement;morphological attributes;polyamines;vitamins;
 Cited by
Abd El-Aziz, N.G., F.E. El-Quesni, and M. Farahat. 2007. Response of vegetative growth and some chemical constituents of Syngonium podophyllum L. to foliar application of thiamine, ascorbic acid and kinetin at Nubaria. World J. Agric. Sci. 3:301-305.

Ainsworth, E.A. and K.M. Gillespie. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat. protoc. 2:875-877. crossref(new window)

Ben Mohamed, H., A.M. Vadel, J. Geuns, and H.Khemira. 2010. Biochemical changes in dormant grapevine shoot tissues in response to chilling: Possible role in dormancy release. Sci. Hortic. 124:440-447. crossref(new window)

Bidwell, R.G.S.1979. Plant physiology. Macmillan Publishing Co., Inc., New York

Blokhina, O., E. Virolainen, and K.V. Fagerstedt. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot. 91:179-194. crossref(new window)

Chapman, H.D. and P.F. Parker. 1961. Methods of analysis for soils, plants and waters. p. 150-196. Univ. California, Div. Agric. Sci., USA.

El-Bassiouny, H. and M. Bekheta. 2004. Role of putrescine on growth, regulation of stomatal aperture, ionic contents and yield by two wheat cultivars under salinity stress. Egypt. J. Physiol. Sci. 2:235-258.

Goldblatt, P. and J.C. Manning. 1998. Gladiolus in Southern Africa: Systematics, biology, and evolution. Fernwood Press, Cape Town.

Farahi, M.H., A. Khalighi, B. Kholdbarin, M.M. Akbar-boojar, and S. Eshghi. 2013. Morphological responses and vase life of Rosa hybrida cv. dolcvitato polyamines spray in hydroponic system. World Appl. Sci. J. 21:1681-1686.

Hanafy, A., M. Gad-Mervat, H. Hassam, and A. Amin-Mona. 2002. Improving growth and chemical composition of Myrtus communis grown under soil salinity conditions by polyamine foliar application. Proc. Minia-Egypt. J. Agric. Res. Dev. 22:1697-1720.

Hanzawa, Y., T. Takahashi, A.J. Michael, J. Burtin, D. Lond, M. Pineiro, G. Coupland, and Y. Komeda. 2000. ACAULISS, an Arabidopsis gene required for stem elongation, encodes spermine synthase. EMBO J. 19:4248-4256. crossref(new window)

Hathout, T.A. 1995. Diverse effect of uniconazole and nicotinamid on germination, growth, endogenous hormones and some enzymatic activity of peas. Egypt. J. Physiol. Sci. 19:77-95.

Kamiab, F., A. Talaie, M. Khezri, and A. Javanshah. 2014. Exogenous application of free polyamines enhance salt tolerance of pistachio (Pistacia vera L.) seedlings. Plant Growth Regul. 72:257-268. crossref(new window)

Lahiji, M.F. 2013. Effect of growth regulators in corm production, growth and development of corm in gladiolus varieties (rose supreme, white prosperity). Int. J. Agron. Plant. Prod. 4:3186-3191.

Lee, M.M., S.H. Lee, and K.Y. Park. 1997. Effects of spermine on ethylene biosynthesis in cut carnation (Dianthus caryophyllus L) flowers during senescence. J. plant physiol. 151:68-73. crossref(new window)

Lichtenthaler, H.K. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Meth. Enzymol. 148:350-382. crossref(new window)

Mahgoub, M., N. Abd El Aziz, and A. Mazhar. 2011. Response of Dahlia pinnata L. plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. Am. Eurasian J. Agric. Environ. Sci. 10:769-775.

Mahgoub, M.H., A. El-Ghorab, and M. Bkheta. 2006. Effect of some bioregulators on the endogenous phytohormones, chemical composition, essential oil and its antioxidant activity of carnation (Dianthus caryophyllus L.). J. Agric. Sci. Mansoura Univ. 31: 4229-4245.

Mahros, K., M. El-Saady, M. Mahgoub, M. Afaf, and M. El-Sayed. 2011. Effect of putrescine and uniconazole treatments on flower characters and photosynthetic pigments of Chrysanthemum indicum. L. plant. J. Am. Sci. 7:399-408.

Maksoud, M., M.A. Saleh, M. El-Shamma, and A.A. Fouad. 2009. The beneficial effect of biofertilizers and antioxidants on olive trees under calcareous soil conditions. World J. Agric. Sci. 5:350-352.

Manning, J. and P. Goldblatt. 2008. The iris family: natural history and classification. Timber Press Inco., Portland, Oregon, USA.

Maurya, R.P. and C.L. Nagda. 2004. Influence of plant growth regulators on growth, flowering and corm yield of gladiolus (Gladiolus grandiflorus L.) cv. Oscar. HortScience 39:823. (Abstr.)

Nahed, G.A.A., S.T. Lobna, and M. Soad. 2009. Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of gladiolus plants at Nubaria. Ozean J. Appl. Sci. 2:169-179.

Nahed, G.A.L.A., F.E.M. El-Quesni, and M.M. Farhat. 2007. Response of vegetative growth and some chemical constituents of Syngonium podophyllum L. to foliar application of thiamine, ascorbic acid and kinetin at Nubaria. World J. Agric. Sci. 3:301-305.

Riazi, A., K. Matsuda, and A. Arslan. 1985. Water-stress induced changes in concentrations of proline and other solutes in growing regions of young barley leaves. J. Exp. Bot. 36:1716-1725. crossref(new window)

Robinson, F.A. 1973. Vitamins, p. 195-220. In: Miller P(ed.). Phytochemistry. Van Reinhold Co., New York.

Sajid, G.M., M. Kaukab, and Z. Ahmad. 2009. Foliar application of plant growth regulators (PGRs) and nutrients for improvement of lily flowers. Pak. J. Bot. 41:233-237.

Salama, K.H.A. 1999. Amelioration of salinity effect in wheat plant by polamines. PhD dissertation, Fac. Ain Shams Univ. Egypt.

Shawky, N.B.T. 2003. Physiological studies on the effect of salinity, ascorbic acid and putrescine on sweet pepper plant. PhD dissertation. Fac. Agric. Cairo Univ. Egypt.

Sudhakar, M. and S.R. Kumar. 2012. Effect of growth regulators on growth, flowering and corm production of gladiolus (Gladiolus grandiflorus L.) cv. White friendship. Ind. J. Pl. Sci. 1:133-136.

Talaat, I.M., M. Bekheta, and M.H. Mahgoub. 2005. Physiological response of periwinkle plants (Catharanthus roseus L.) to tryptophan and putrescine. Int. J. Agric. Biol. 7:210-213.

Talaat, N.B. 2003. Physiological studies on the effect of salinity, ascorbic acid and putrescine of sweet pepper plant. PhD dissertation, Fac. Agric. Cairo Univ. Egypt.

Tarraf, S.A., K.G. El-Din, and L.K. Balbaa. 1999. The response of vegetative growth, essential oil of lemongrass (Cymbopom citrates Hort.) to foliar application of ascorbic acid, nicotenamid and some micronutrients. Arab Univ. J. Agric. Sci. 7:247-259.

USDA. 2012. National Agricultural Statistics Service. Floricultural crops summary. New Jersey, USA.

Youssef, A., R. El-Mergawi, and M.A. El-Wahed. 2004. Effect of putrescine and phenylalanine on growth and alkaloid production of some Daturaspecies. J. Agric. Sci. Mansoura Univ. 29:4037-4057.

Youssef, A. and I. Talaat. 2003. Physiological response of rosemary plants to some vitamins. Egypt. Pharm. J. 1:81-93.