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The Selection of Domestically Bred Cultivars for Spray-type Chrysanthemum Transformation
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 Title & Authors
The Selection of Domestically Bred Cultivars for Spray-type Chrysanthemum Transformation
Suh, Eun-Jung; Han, Bong Hee; Lee, Yeon-Hee; Lee, Seong-Kon; Hong, Joon Ki; Kim, Kyung Hwan;
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 Abstract
To select suitable spray chrysanthemum cultivars for Agrobacterium-mediated transformation, thirty-nine (39) spray cultivars bred in the National Institutes of Korea and a standard cultivar Jinba from Japan were collected and tested for regeneration rate and Agrobacterium infection assays. MS medium with IAA and BAP was used for shoot regeneration from leaf disks and internodes. The shoot regeneration rate in leaf disks was the highest in cultivar BRM, followed by cultivars VS, WW and YTM. The cultivar JB (Jinba) used as a transformation material in previous reports ranked similarly to cultivars PK and SPP. In shoot regeneration from internodes, the shoot regeneration rate was the highest for cultivar PA, followed by cultivar WW. The infection rate of leaves and internodes of 40 chrysanthemum cultivars with agrobacterium was investigated. Cultivars WPP, YNW, VS, PP, WW, FA, PA and YMN showed the highest infection levels in leaves, whereas cultivars WPP, PA, PK and YNW had the highest infection levels in internodes. Considering all of these results, cultivars VS and WW were the most appropriate for gene transformation of chrysanthemum using leaves, while cultivar PA was for internodes.
 Keywords
spray-type chrysanthemum;regeneration;agrobacterium;transformation;GUS (beta-glucuronidase);
 Language
Korean
 Cited by
1.
주요 스프레이 국화 품종의 형태적 특성과 변이계수, 유전율 및 유전자 전이율,심성임;임기병;김창길;정미영;김경민;정재동;

원예과학기술지, 2016. vol.34. 2, pp.269-281 crossref(new window)
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