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Production of Adventitious Root and Analysis of Effective Components from in vitro Culture of Astragalus membranaceus
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 Title & Authors
Production of Adventitious Root and Analysis of Effective Components from in vitro Culture of Astragalus membranaceus
Hur, Mok; Lee, Dae Young; Lee, Jae Won; An, Tae Jin; Lee, Jeong Hoon; Kim, Young Guk; Cha, Seon Woo; Um, Yurry;
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Background : A series of studies were conducted to optimize adventitious root induction in vitro from explants of Astragalus membranaceus using various nutrient media supplemented with plant hormones. Methods and Results : Levels of active components were analyzed from adventitious roots induced under different media conditions. Among the different media conditions, Murashige and Skoog medium supplemented with indole-3-butyric acid resulted in the greatest adventitious root induction rate. The amount of the major active component of the adventitious roots of Ama1, calycosin-7-O--D-glucopyranoside was higher than that of other adventitious root samples. Conclusions : These results suggest that the adventitious roots of A. membranaceus could be used for the commercial production of medicines.
Astragalus membranaceus;Adventitious Roots;Calycosin-7-O--D-Glucopyranoside;
 Cited by
Baek NI, Kim YS, Kyung JS and Park KH. (1996). Isolation of antihepatotoxic from the root of Astragalus membranceus. Korean Journal of Pharmacognosy. 27:111-116.

Cho WCS and Leung KN. (2007). In vitro and in vivo antitumor effects of Astragalus membranaceus. Cancer Letters. 252:43-54. crossref(new window)

Choi S, Park SR and Heo TR. (2005). Inhibitory effect of Astragali radix on matrix degradation in human articular cartilage. Journal of Microbiology and Biotechnology. 15:1258-1266.

Choi SI, Heo TR, Min BH, Cui JH, Choi BH and Park SR. (2007). Alleviation of osteoarthritis by calycosin-7-O-$\beta$-Dglucopyranoside( CG) isolated from Astragali radix(AR) in rabbit osteoarthritis(OA) model. Osteoarthritis and Cartilage. 15:1086-1092. crossref(new window)

Dong TTX, Ma XQ, Clarke C, Song ZH, Ji ZN, Lo CK and Tsim KWK. (2003). Phylogeny of Astragalus in China: Molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA. Journal of Agricultural and Food Chemistry. 51:6709-6714. crossref(new window)

Du M, Wu XJ, Ding J, Hu ZB, White KN and Branford- White CJ. (2003). Astragaloside IV and polysaccharides production by hairy roots of Astragalus membranaceus in bioreactors. Biotechnology Letters. 25:1853-1856. crossref(new window)

Du XG, Zhao B, Li JY, Cao XH, Diao MK, Feng HB, Chen XB, Chen ZY and Zeng XY. (2012). Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice. International Immunopharmacology. 14:463-470. crossref(new window)

Hirotani M, Zhou Y, Lui H and Furuya T. (1994). Astragalosides from hairy root cultures of Astragalus membranaceus. Phytochemistry. 36:665-670. crossref(new window)

Ionkova I, Momekov G and Proksch P. (2010). Effects of cycloartane saponins from hairy roots of Astragalus membranaceus Bge., on humantumor cell targets. Fitoterapia. 81:447-451. crossref(new window)

Jang YS, Cui HY, Lee EJ, Kim HW and Paek KY. (2012). Auxin affects on production of adventitious roots and secondary metabolites in Echinacea angustifolia. Korean Journal of Medicinal Crop Science. 20:479-486. crossref(new window)

Jeong CS, Chakrabarty D, Hahn EJ, Lee HL and Paek KY. (2006). Effects of oxygen, carbon dioxide and ethylene on growth and bioactive compound production in bioreactor culture of ginseng adventitious roots. Biochemical Engineering Journal. 27:252-263. crossref(new window)

Kajimura K, Takagi Y, Ueba N, Yamasaki K, Sakagami Y, Yokoyama H and Yoneda K. (1996). Protective effect of Astragali radix by intraperitoneal injection against Japanese encephalitis virus infection in mice. Biological and Pharmaceutical Bulletin. 19:855-859. crossref(new window)

Kim EJ, Oh OJ, Lee SK and Yang KS. (2001) Inhibitory effect of Astragali Radix on COX-2 activity. Korean Journal of Pharmacognosy. 32:311-315.

Kim GS, Lee DY, Lee SE, Noh HJ, Choi JH, Park CG, Choi SI, Hong SJ and Kim SY. (2013). Evaluation on extraction conditions and HPLC analysis method for bioactive compounds of Astragali radix. Korean Journal of Medicinal Crop Science. 21:486-492. crossref(new window)

Kim JH, Park SY, Lim HK, Park AY, Kim JS, Kang SS, Youm JR and Han SB. (2007). Quantitative evaluation of radix Astragali through the simultaneous determination of bioactive isoflavonoids and saponins by HPLC/UV and LCESI- MS/MS. Bulletin of the Korean Chemical Society. 28:1187-1194. crossref(new window)

Kim JS and Kim CS. (1997). A study on the constituents from the roots of Astragalus membranaceus Bunge (II). Korean Journal of Pharmacognosy. 28:75-79.

Kim JS and Kim CS. (2000). A study on the constituents from the roots of Astragalus membranaceus Bunge (III). Korean Journal of Pharmacognosy. 31:109-111.

Kim SH, Jun YM, Lim JJ, Kim SH, Chung IM and Kim EH. (2012a). Variation of astragalosides contents in cultivated Astragalus membranaceus. Korean Journal of Medicinal Crop Science. 20:372-380. crossref(new window)

Kim SP, Kim SM, Ryu HS, Shin JC, Lee DG, Lee OJ, Lee JH and Kim JH. (2012b). The effects of Astraglai radix pharmacopuncture at CV12 on osteoporosis of senescence accelerated mice(SAM) P6. The Journal of Korean Acupuncture and Moxibustion Medicine Society. 29:59-71.

Kim YS, Chakrabarty D, Hahn EJ and Paek KY. (2003). Methyl jasmonate increases saponin content in bioreactor culture of ginseng(Panax ginseng C. A. Meyer) adventitious roots. Acta Horticulturae. 625:289-292.

Lee BS, In JG, Song WS and Yang DC. (2004). Increase of ginsenosides production by the treatment of chitosan and jasmonic acid in the adventitious roots of Korean ginseng(Panax ginseng C.A. Meyer). Korean Journal of Plant Resources. 17:48-53.

Lee DY, Noh HJ, Choi JH, Lee KH, Lee MH, Lee JH, Hong YP, Lee SE, Kim SY and Kim GS. (2013). Anti-inflammatory cycloartane type saponins of Astragalus membranaceus. Molecules. 18:3725-3732. crossref(new window)

Lee YS, Han OK, Park CW, Suh SI, Shin SW, Yang CH, Jeon TW, Lee ES, Kim KJ, Kim SH, Yoo WK and Kim HJ. (2003). Immunomodulatory effects of aqueous extracted Astragali radix in methotrexate treated mouse spleen cells. Journal of Ethnopharmacology. 84:193-198. crossref(new window)

Lei H, Wang B, Li WP, Yang Y, Zhou AW and Chen MZ. (2003). Anti-aging effect of astragalosides and its mechanism of action. Acta Pharmacologica Sinica. 24:230-234.

Liu J, Zhao ZZ and Chen HB. (2011). Review of Astragali radix. Chinese Herbal Medicines. 3:90-105.

Lu JF, Li CX, Muteliefu G, Li TF, Tu PF, Yin JJ and Cai SQ. (2002). Effects of BYHW decoction and its effective constituents on the fluidity of the cell membrane in a stroke modeled rat brain. Journal of Chinese Pharmaceutical Sciences. 11:132-136.

Ma XQ, Duan JA, Zhu DY, Dong TX and Tsim KWK. (2000). Chemical comparison of Astragali radix(Huangqi) from different regions of China. Natural Medicines. 54:213-218.

Murashige T and Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. 15:473-497. crossref(new window)

Park YC, Lee JS, Kim DY, Son HY, Lee JW, Cheoi YS, Kim KK, Yu CY, Chung IM, Im MH, Lee KJ, Choi RN, Shim HS and Lim JD. (2013). A 90 day repeated dose oral toxicity study of extracts from Astragalus membranaceus aboveground parts in rats. Korean Journal of Medicinal Crop Science. 21:474-485. crossref(new window)

Paek KY and Hahn EJ. (2005). Increase of saponin contents via elicitor treatments in bioreactor culture of ginseng(Panax ginseng C. A. Meyer) adventitious roots. Acta Horticulturae. 679:145-148.

Sivakumar G, Yu KW and Paek KY. (2006). Enhanced production of bioactive ginsenosides from adventitious roots of Panax ginseng in bioreactor culture. Journal of Horticultural Science and Biotechnology. 81:549-552. crossref(new window)

Staswick PE. (1992). Jasmonate, genes, and fragrant signals. Plant Physiology. 99:804-807. crossref(new window)

Thwe AA, Mai NTT, Li X, Kim Y, Kim YB, Uddin MR, Kim YS, Bae H, Kim HH, Lee MY and Park SU. (2012). Production of astragaloside and flavones from adventitious root cultures of Astragalus membranaceus var. mongholicus. Plant Omics Journal. 5:466-470.

Wang JH, Kong J, Li W, Molchanova V, Chikalovets I, Belogortseva N, Luk'yanov P and Zheng YT. (2006). A betagalactose- specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology. 142:111-117. crossref(new window)

Wang XJ and Feng P. (2000). Antioxidant activity of Qizhu tang. Acta Pharmacologica Sinica. 21:1141-1144.

Wu SQ, Lian ML, Gao R, Park SY and Piao XC. (2011). Bioreactor application on adventitious root culture of Astragalus membranaceus. In Vitro Cellular and Developmental Biology Plant. 47:719-724. crossref(new window)

Yoshikawa T and Furuya T. (1987). Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Reports. 6:449-453.

Yu DH, Duan YL, Bao YM, Wei CL and An LJ. (2005). Isoflavonoids from Astragalus mongholicus protect PC12 cells from toxicity induced by L-glutamate. Journal of Ethnopharmacology. 98:89-94. crossref(new window)

Zhang WJ, Hufnagl P, Binder BR and Wojta J. (2003). Antiinflammatory activity of astragaloside IV is mediated by inhibition of NF-kappa B activation and adhesion molecule expression. Thrombosis and Haemostasis. 90:904-914.

Zhang YD, Wang YL, Shen JP and Li DX. (1984). Effect of astragaloside IV on inflammatory and hypertension. Acta Pharmacologica Sinica. 19:333-337.