Advanced SearchSearch Tips
Internal Transcribed Spacer Barcoding DNA Region Coupled with High Resolution Melting Analysis for Authentication of Panax Species
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Internal Transcribed Spacer Barcoding DNA Region Coupled with High Resolution Melting Analysis for Authentication of Panax Species
Bang, Kyong Hwan; Kim, Young Chang; Lim, Ji Young; Kim, Jang Uk; Lee, Jung Woo; Kim, Dong Hwi; Kim, Kee Hong; Jo, Ick Hyun;
  PDF(new window)
Background : Correct identification of Panax species is important to ensure food quality, safety, authenticity and health for consumers. This paper describes a high resolution melting (HRM) analysis based method using internal transcribed spacer (ITS) and 5.8S ribosomal DNA barcoding regions as target (Bar-HRM) to obtain barcoding information for the major Panax species and to identify the origin of ginseng plant. Methods and Results : A PCR-based approach, Bar-HRM was developed to discriminate among Panax species. In this study, the ITS1, ITS2, and 5.8S rDNA genes were targeted for testing, since these have been identified as suitable genes for use in the identification of Panax species. The HRM analysis generated cluster patterns that were specific and sensitive enough to detect small sequence differences among the tested Panax species. Conclusion : The results of this study show that the HRM curve analysis of the ITS regions and 5.8S rDNA sequences is a simple, quick, and reproducible method. It can simultaneously identify three Panax species and screen for variants. Thus, ITS1HRM and 5.8SHRM primer sets can be used to distinguish among Panax species.
Panax Species;High Resolution Melting;ITS Barcoding;Real Time PCR;
 Cited by
Analysis of the chloroplast genome and SNP detection in a salt tolerant breeding line in Korean ginseng,;;;;;;;;;

Journal of Plant Biotechnology, 2016. vol.43. 4, pp.417-421 crossref(new window)
Analysis of the chloroplast genome and SNP detection in a salt tolerant breeding line in Korean ginseng, Journal of Plant Biotechnology, 2016, 43, 4, 417  crossref(new windwow)
Baldwin BG. (1992). Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the compositae. Molecular Phylogenetics and Evolution. 1:3-16. crossref(new window)

Bang KH, Jo IH, Kim YC, Kim JU, Park HW, Shin MR, Kim YB, Kim OT, Hyun DY, Kim DH and Cha SW. (2012). Molecular identification of Korean ginseng cultivars(Panax ginseng C. A. Mey.) using peptide nucleic acid(PNA) microarray. Korean Journal of Medicinal Crop Science. 20:387-392. crossref(new window)

Bosmali I, Ganopoulos I, Madesis P and Tsaftaris A. (2012). Microsatellite and DNA-barcode regions typing combined with high resolution melting(HRM) analysis for food forensic uses: A case study on lentils(Lens culinaris). Food Research International. 46:141-147. crossref(new window)

Chan TW, But PP, Cheng SW, Kwok IM, Lau FW and Xu HX. (2000). Differentiation and authentication of Panax ginseng, Panax quinquefolius, and ginseng products by using HPLC/MS. Analytical Chemistry. 72:1281-1287. crossref(new window)

Choi HK and Wen J. (2000). A phylogenetic analysis of Panax (Araliaceae): Integrating cpDNA restriction site and nuclear rDNA ITS sequence data. Plant Systematics and Evolution. 224:109-120. crossref(new window)

Cui JF, Eneroth P and Bruhn JB. (1999). Gynostemma pentaphyllum: Identification of major sapogenins and differentiation from Panax species. European Journal of Pharmaceutical Sciences. 8:187-191. crossref(new window)

Ganopoulos I, Bazakos C, Madesis P, Kalaitzis P and Tsaftaris A. (2013). Barcode DNA high resolution melting(Bar-HRM) analysis as a novel close-tubed and accurate tool for olive oil forensic use. Journal of the Science of Food and Agriculture. 93:2281-2286. crossref(new window)

Ganopoulos I, Madesis P and Tsaftaris A. (2012). Universal ITS2 barcoding DNA region coupled with high resolution melting(HRM) analysis for seed authentication and adulteration testing in Leguminous forage and pasture species. Plant Molecular Biology Reporter. 30:1322-1328. crossref(new window)

Jaakola L, Suokas M and Hggman H. (2010). Novel approaches based on DNA barcoding and high resolution melting of amplicons for authenticity analyses of berry species. Food Chemistry. 123:494-500. crossref(new window)

Jo IH, Kim YC, Kim JU, Lee SH, Lim JY, Moon JY, Noh BS, Hyun DY, Kim DH, Kim KH and Bang KH. (2014). A rapid identification of Korean ginseng cultivar, Cheonryang, using specific DNA markers. Korean Journal of Medicinal Crop Science. 22:429-434. crossref(new window)

Kalivas A, Ganopoulos I, Xanthopoulou A, Chatzopoulou P, Tsaftaris A and Madesis P. (2014). DNA barcode ITS2 coupled with high resolution melting(HRM) analysis for taxonomic identification of Sideritis species growing in Greece. Molecular Biology Reports. 41:5147-5155. crossref(new window)

Kang J, Lee S, Kang S, Kwon HN, Park JH, Kwon SW and Park S. (2008). NMR-based metabolomics approach for the differentiation of ginseng(Panax ginseng) roots from different origins. Archives of Pharmacal Research. 31:330-336. crossref(new window)

Kim OT, Bang KH, In DS, Lee JW, Kim YC, Shin YS, Hyun DY, Lee SS, Cha SW and Seong NS. (2007). Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences. Plant Biotechnology Reports. 1:163-167. crossref(new window)

Lee OR, Kim MK and Yang DC. (2012). Authentication of medicinal plants by SNP-based multiplex PCR. Methods in Molecular Biology. 862:135-147. crossref(new window)

Li WK and Fitzloff JF. (2001). A validated method for quantitative determination of saponins in notoginseng(Panax notoginseng) using high performance liquid chromatography with evaporative light scattering detection. Journal of Pharmacy and Pharmacology. 53:1637-1643. crossref(new window)

Madesis P, Ganopoulos I, Anagnostis A and Tsaftaris A. (2012). The application of Bar-HRM(Barcode DNA-High Resolution Melting) analysis for authenticity testing and quantitative detection of bean crops(Leguminosae) without prior DNA purification. Food Control. 25:576-582. crossref(new window)

Mao Q, Bai M, Xu JD, Kong M, Zhu LY, Zhu H, Wang Q and Li SL. (2014). Discrimination of leaves of Panax ginseng and P. quinquefolius by ultra high performance liquid chromatography quadrupole/time of-flight mass spectrometry based metabolomics approach. Journal of Pharmaceutical and Biomedical Analysis. 97:129-140. crossref(new window)

Park MJ, Kim MK, In JG and Yang DC. (2006). Molecular identification of Korean ginseng by amplification refractory mutation system-PCR. Food Research International. 39:568-574. crossref(new window)

Shaw PC and But PP. (1995). Authentication of Panax species and their adulterants by random primed polymerase chain reaction. Planta Medica. 61:466-469. crossref(new window)

Stephens AJ, Inman-Bamber J, Giffard PM and Huygens F. (2008). High resolution melting analysis of the spa repeat region of Staphylococcus aureus. Clinical Chemistry. 54:432-436. crossref(new window)

Wang H, Kim MK, Kwon WS, Jin H, Liang Z and Yang DC. (2011). Molecular authentication of Panax ginseng and ginseng products using robust SNP markers in ribosomal external transcribed spacer region. Journal of Pharmaceutical and Biomedical Analysis. 55:972-976. crossref(new window)

Wen J and Zimmer EA. (1996). Phylogeny and biogeography of Panax L.(the ginseng genus, Araliaceae): Inferences from ITS sequences of nuclear ribosomal DNA. Molecular Phylogenetics and Evolution. 6:167-177. crossref(new window)

Wittwer CT, Reed GH, Gundry CN, Vandersteen JG and Pryor RJ. (2003). High resolution genotyping by amplicon melting analysis using LCGreen. Clinical Chemistry. 49:853-860. crossref(new window)

Yang SO, Lee SW, Kim YO, Lee SW, Kim NH, Choi HK, Jung JY, Lee DH and Shin YS. (2014). Comparative analysis of metabolites in roots of Panax ginseng obtained from different sowing methods. Korean Journal of Medicinal Crop Science. 22:17-22. crossref(new window)

Yang WZ, Bo T, Ji S, Qiao X, Guo DA and Ye M. (2013). Rapid chemical profiling of saponins in the flower buds of Panax notoginseng by integrating MCI gel column chromatography and liquid chromatography/mass spectrometry analysis. Food Chemistry. 139:762-769. crossref(new window)

Yao H, Song J, Liu C, Luo K, Han J, Li Y, Pang X, Xu H, Zhu Y, Xiao P and Chen S. (2010). Use of ITS2 region as the universal DNA barcode for plants and animals. PLoS ONE. 5:e13102. crossref(new window)

Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais B and Wittwer CT. (2004). High resolution DNA melting curve analysis to establish HLA genotype identity. Tissue Antigens. 64:156-164. crossref(new window)