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Internal Transcribed Spacer Barcoding DNA Region Coupled with High Resolution Melting Analysis for Authentication of Panax Species
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 Title & Authors
Internal Transcribed Spacer Barcoding DNA Region Coupled with High Resolution Melting Analysis for Authentication of Panax Species
Bang, Kyong Hwan; Kim, Young Chang; Lim, Ji Young; Kim, Jang Uk; Lee, Jung Woo; Kim, Dong Hwi; Kim, Kee Hong; Jo, Ick Hyun;
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 Abstract
Background : Correct identification of Panax species is important to ensure food quality, safety, authenticity and health for consumers. This paper describes a high resolution melting (HRM) analysis based method using internal transcribed spacer (ITS) and 5.8S ribosomal DNA barcoding regions as target (Bar-HRM) to obtain barcoding information for the major Panax species and to identify the origin of ginseng plant. Methods and Results : A PCR-based approach, Bar-HRM was developed to discriminate among Panax species. In this study, the ITS1, ITS2, and 5.8S rDNA genes were targeted for testing, since these have been identified as suitable genes for use in the identification of Panax species. The HRM analysis generated cluster patterns that were specific and sensitive enough to detect small sequence differences among the tested Panax species. Conclusion : The results of this study show that the HRM curve analysis of the ITS regions and 5.8S rDNA sequences is a simple, quick, and reproducible method. It can simultaneously identify three Panax species and screen for variants. Thus, ITS1HRM and 5.8SHRM primer sets can be used to distinguish among Panax species.
 Keywords
Panax Species;High Resolution Melting;ITS Barcoding;Real Time PCR;
 Language
English
 Cited by
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Analysis of the chloroplast genome and SNP detection in a salt tolerant breeding line in Korean ginseng,;;;;;;;;;

Journal of Plant Biotechnology, 2016. vol.43. 4, pp.417-421 crossref(new window)
1.
Analysis of the chloroplast genome and SNP detection in a salt tolerant breeding line in Korean ginseng, Journal of Plant Biotechnology, 2016, 43, 4, 417  crossref(new windwow)
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