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Morphological Characteristics and Genetic Diversity Analysis of Platycodon grandiflorum (Jacq.) A. DC Determined Using SSR Markers
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 Title & Authors
Morphological Characteristics and Genetic Diversity Analysis of Platycodon grandiflorum (Jacq.) A. DC Determined Using SSR Markers
Um, Yurry; Lee, Yi; Jin, Mei-Lan; Lee, Dae Young; Lee, Jae Won; Kim, Geum Soog; Kim, Chang Kug; Hong, Chang Pyo; Kim, Ok Tae;
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 Abstract
Background : Plant breeding requires the collection of genetically diverse genetic resources. Studies on the characteristics of Platycodon grandiflorum resources have not been carried out so far. The present study was carried out to discriminate P. grandiflorum based on morphological characteristics and genetic diversity using simple sequence repeat (SSR) markers. Methods and Results :We collected 11 P. grandiflorum cultivars: Maries II, Hakone double white, Hakone double blue, Fuji white, Fuji pink, Fuji blue, Astra white, Astra pink, Astra blue, Astra semi-double blue and Jangbaek. Analyses of the morphological characteristics of the collection were conducted for aerial parts (flower, stem and leaf) and underground parts (root). Next, the genetic diversity of all P. grandiflorum resources was analyzed using SSR markers employing the DNA fragment analysis method. We determined that the 11 P. grandiflorum cultivars analyzed could be classified by plant length, leaf number and root characteristic. Based on the genetic diversity analysis, these cultivars were classified into four distinct groups. Conclusions : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of P. grandiflorum. Moreover, the markers could be used for genetic mapping of the plant and marker-assisted selection for crop breeding.
 Keywords
Platycodon grandiflorum;Morphological Characteristics;Genetic Diversity;Simple Sequence Repeats;
 Language
Korean
 Cited by
1.
Fluorescence in situ Hybridization Karyotype Analysis of Seven Platycodon grandiflorum (Jacq.) A. DC. Cultivars,;;;;;

원예과학기술지, 2017. vol.35. 6, pp.784-792 crossref(new window)
 References
1.
Ahn KS, Noh EJ, Zhao HL, Jung SH, Kang SS and Kim YS. (2005). Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-kB activation in RAW 264.7 cells. Life Sciences. 76:2315-2328. crossref(new window)

2.
Choi CY, Kim JY, Kim YS, Chung YC, Seo JK and Jeong HG. (2001). Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor ecrosis factor-${\alpha}$ from murine macrophages. International Immunopharmacology. 1:1141-1151. crossref(new window)

3.
Han EH, Son YW, Kim MB, Shin YW, Cho YS and Lee SW. (2014). Establishment of tissue culture and acclimation of white balloon flower(Platycodon grandiflorum DC. cv. Jangback) for the raising of in vitro propagated seedlings. Journal of Plant Biotechnology. 41:134-139. crossref(new window)

4.
Jo IH, Bang KH, Kim YC, Kim JU, Shin MR, Moon JY, Noh BS, Hyun DY, Kim DH, Cha SW and Kim HS. (2013). Analysis of mitochondrial DNA sequence and molecular marker development for identification of Panax species. Korean Journal of Medicinal Crop Science. 21:91-96. crossref(new window)

5.
Kim JH, Seo JW, Byeon JH, Ahn YS, Cha SW and Cho JH. (2014). Morphological characteristics and phylogenetic analysis of Polygonatum species based on chloroplast DNA sequences. Korean of Journal Medicinal Crop Science. 22:489-496. crossref(new window)

6.
Korea Seed and Variety Service(KSVS). (2000). Variety characteristics of test guideline. Ministry of Agriculture, Food and Rural Affairs. Gimcheon, Korea. p.1-15.

7.
Lee GA, Song JY, Sung JS, Choi YM, Lee JR, Lee SY, Kim CY, Kim YG and Lee MC. (2012). Analysis of population structure and genetic diversity in balloon flower Platycodon grandiflorum(Jacq.) A. DC. germplasm using simple sequence repeat(SSR) markers. Journal of Crop Science and Biotechnology. 15:281-287. crossref(new window)

8.
Lee HY, Kang RH, Kim YS, Chung SI and Yoon YS. (2010). Platycodin D inhibits adipogenesis of 3T3 L1 cells by modulating kruppel like factor 2 and peroxisome proliferator activated receptor gamma. Phytotherapy Research. 24:S161-S167. crossref(new window)

9.
Li J, Schulz B and Stich B. (2010). Population structure and genetic diversity in elite sugar beet germplasm investigated with SSR markers. Euphytica. 175:35-42. crossref(new window)

10.
Mabberley DJ. (1987). The plant-book: A portable dictionary of the higher plants. Cambridge University Press. Cambridge, England. p.461.

11.
Ministry of Agriculture, Food and Rural Affairs(MAFRA). (2014). Production performance of industrial crops. Ministry of Agriculture, Food and Rural Affairs. Sejong, Korea. p.28.

12.
Moon BC, Kim WJ, Ji Y, Lee YM and Kim HK. (2013). Genetic diversity of Curcuma genus collected germplasm using analysis of AFLP. Korean Journal of Medicinal Crop Science. 21:455-460. crossref(new window)

13.
Murashige T and Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. 15:473-497. crossref(new window)

14.
Park CG, Bang KH, Kim OT, Jin DC, Kim DH, Sung JS, Seong NS, Park HW and Lee SC. (2007). Development of SCAR marker for discriminating between violet flowered lines and white flowered lines in Chinese bellflower(Platycodon grandiflorum A.). Korean Journal of Medicinal Crop Science. 15:1-5.

15.
Saxena B, Kaur R and Bhardwaj SV. (2011). Assessment of genetic diversity in cabbage cultivars using RAPD and SSR markers. Journal of Crop Science and Biotechnology. 14:191-196. crossref(new window)

16.
Sirithunya P and Ukoskit K. (2010). Population genetic structure and genetic diversity of Jatropha curcas germplasm as investigated by 5'-anchored simple sequence repeat primers. Journal of Crop Science and Biotechnology. 13:147-153. crossref(new window)

17.
Song JY, Lee GA, Yoon MS, Ma KH, Choi YM, Lee JR, Park HJ and Lee MC. (2012). Development and characterization of 22 polymorphic microsatellite markers for the balloon flower Platycodon grandiflorum(Campanulaceae). Genetics and Molecular Research. 11:3263-3266. crossref(new window)

18.
Vigouroux Y, Mitchell S, Matsuoka Y, Hamblin M, Kresovich S, Smith JSC, Jaqueth J, Smith OS and Doebley J. (2005). An analysis of genetic diversity across the maize genome using microsatellites. Genetics. 169:1617-1630. crossref(new window)

19.
Wang C, Levis GBS, Lee EB, Levis WR, Lee DW, Kim BS, Park SY and Park E. (2004). Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-${\alpha}$ in activated RAW 264.7 cells. International immunopharmacology. 4:1039-1049. crossref(new window)

20.
Williams JGK, Kubelik AR, Livak KJ, Raafalski JA and Tingey SV. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research. 18:6531-6535. crossref(new window)

21.
Zhao HL, Cho KH, Ha YW, Jeong TS, Lee WS and Kim YS. (2006). Cholesterol-lowing effect of platycodin D in hyper cholesterol emic ICR mice. European Journal of Pharmacology. 537:166-173. crossref(new window)