JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Biomolecules and Therapeutics
  • Volume 24, Issue 1,  2016, pp.94-98
  • Publisher : The Korean Society of Applied Pharmacology
  • DOI : 10.4062/biomolther.2015.183
 Title & Authors
Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells)
Lee, Na-Young; Park, Hyun-Joo; Kang, Young-Sook;
  PDF(new window)
 Abstract
The objective of the present study was to elucidate the effect of bisphosphonates, anti-osteoporosis agents, on glucose uptake in retinal capillary endothelial cells under normal and high glucose conditions. The change of glucose uptake by pre-treatment of bisphosphonates at the inner blood-retinal barrier (iBRB) was determined by measuring cellular uptake of -O-methyl glucose (3-OMG) using a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB cells) under normal and high glucose conditions. -OMG uptake was inhibited by simultaneous treatment of unlabeled D-glucose and 3-OMG as well as glucose transport inhibitor, cytochalasin B. On the other hand, simultaneous treatment of alendronate or pamidronate had no significant inhibitory effect on -OMG uptake by TR-iBRB cells. Under high glucose condition of TR-iBRB cells, -OMG uptake was increased at 48 h. However, -OMG uptake was decreased significantly by pre-treatment of alendronate or pamidronate compared with the values for normal and high glucose conditions. Moreover, geranylgeraniol (GGOH), a mevalonate pathway intermediate, increased the uptake of -OMG reduced by bisphosphonates pre-treatment. But, pre-treatment of histamine did not show significant inhibition of -OMG uptake. The glucose uptake may be down regulated by inhibiting the mevalonate pathway with pre-treatment of bisphosphonates in TR-iBRB cells at high glucose condition.
 Keywords
Glucose uptake;Bisphosphonates;Inner blood-retinal barrier;Retinal capillary endothelial cells;Mevalonate pathway;
 Language
English
 Cited by