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In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It`s Fractions on Leukemia HL-60 Cells
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 Title & Authors
In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It`s Fractions on Leukemia HL-60 Cells
Diab, Kawthar AE; Shafik, Reham Ezzat; Yasuda, Shin;
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 Abstract
In the present work, novel orange peel was extracted with 100%EtOH (ethanol) and fractionated into four fractions namely F1, F2, F3, F4 which were eluted from paper chromatographs using 100%EtOH, 80%EtOH, 50%EtOH and pure water respectively. The crude extract and its four fractions were evaluated for their total polyphenol content (TPC), total flavonoid content (TFC) and radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Their cytotoxic activity using WST assay and DNA damage by agarose gel electrophoresis were also evaluated in a human leukemia HL-60 cell line. The findings revealed that F4 had the highest TPC followed by crude extract, F2, F3 and F1. However, the crude extract had the highest TFC followed by F4, F3, F2, and F1. Depending on the values of and trolox equivalent antioxidant capacity, F4 possessed the strongest antioxidant activity while F1 and F2 displayed weak antioxidant activity. Further, incubation HL-60 cells with extract/fractions for 24h caused an inhibition of cell viability in a concentration-dependent manner. F3 and F4 exhibited a high antiproliferative activity with a narrow range of values (). Crude extract exhibited the weakest antiproliferative activity with an value of . Analysis of DNA fragmentation displayed DNA degradation in the form of a smear-type pattern upon agarose gel after incubation of HL-60 cells with F3 and F4 for 6 h. Overall, F3 and F4 appear to be good sources of phytochemicals with antioxidant and potential anticancer activities.
 Keywords
Novel orange peel;total polyphenol content;total flavonoid content;DPPH assay;HL-60 cells;CCK-8;
 Language
English
 Cited by
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In Vitro Studies on Phytochemical Content, Antioxidant, Anticancer, Immunomodulatory, and Antigenotoxic Activities of Lemon, Grapefruit, and Mandarin Citrus Peels,;

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