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Correlation between EGFR Gene Mutations and Lung Cancer: a Hospital-Based Study
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 Title & Authors
Correlation between EGFR Gene Mutations and Lung Cancer: a Hospital-Based Study
Kavitha, Matam; Iravathy, Goud; Adi Maha, Lakshmi M; Ravi, V; Sridhar, K; Vijayanand, Reddy P; Chakravarthy, Srinivas; Prasad, SVSS; Tabassum, Shaik Nazia; Shaik, Noor Ahmad; Syed, Rabbani; Alharbi, Khalid Khalaf; Khan, Imran Ali;
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 Abstract
Epidermal growth factor receptor (EGFR) is one of the targeted molecular markers in many cancers including lung malignancies. Gefitinib and erlotinib are two available therapeutics that act as specific inhibitors of tyrosine kinase (TK) domains. We performed a case-control study with formalin-fixed paraffin-embedded tissue blocks (FFPE) from tissue biopsies of 167 non-small cell lung carcinoma (NSCLC) patients and 167 healthy controls. The tissue biopsies were studied for mutations in exons 18-21 of the EGFR gene. This study was performed using PCR followed by DNA sequencing. We identified 63 mutations in 33 men and 30 women. Mutations were detected in exon 19 (delE746-A750, delE746-T751, delL747-E749, delL747-P753, delL747-T751) in 32 patients, exon 20 (S786I, T790M) in 16, and exon 21 (L858R) in 15. No mutations were observed in exon 18. The 63 patients with EFGR mutations were considered for upfront therapy with oral tyrosine kinase inhibitor (TKI) drugs and have responded well to therapy over the last 15 months. The control patients had no mutations in any of the exons studied. The advent of EGFR TKI therapy has provided a powerful new treatment modality for patients diagnosed with NSCLC. The study emphasizes the frequency of EGFR mutations in NSCLC patients and its role as an important predictive marker for response to oral TKI in the south Indian population.
 Keywords
EFGR;NSCLC;TKI;DNA sequencing;
 Language
English
 Cited by
1.
EGFR Mutation Genotype Impact on the Efficacy of Pemetrexed in Patients with Non-squamous Non-small Cell Lung Cancer,;;;;;;;;;;;

Asian Pacific Journal of Cancer Prevention, 2016. vol.17. 7, pp.3249-3253 crossref(new window)
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Prevalence and Clinical Profile of EGFR Mutation In Non-Small-Cell Lung Carcinoma Patients in Southwest China, Asian Pacific Journal of Cancer Prevention, 2016, 17, 3, 965  crossref(new windwow)
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