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Shikonin Induced Necroptosis via Reactive Oxygen Species in the T-47D Breast Cancer Cell Line
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 Title & Authors
Shikonin Induced Necroptosis via Reactive Oxygen Species in the T-47D Breast Cancer Cell Line
Shahsavari, Zahra; Karami-Tehrani, Fatemeh; Salami, Siamak;
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Breast cancer, the most common cancer in the women, is the leading cause of death. Necrotic signaling pathways will enable targeted therapeutic agents to eliminate apoptosis-resistant cancer cells. In the present study, the effect of shikonin on the induction of cell necroptosis or apoptosis was evaluated using the T-47D breast cancer cell line. The cell death modes, caspase-3 and 8 activities and the levels of reactive oxygen species (ROS) were assessed. Cell death mainly occurred through necroptosis. In the presence of Nec-1, caspase-3 mediated apoptosis was apparent in the shikonin treated cells. Shikonin stimulates ROS generation in the mitochondria of T-47D cells, which causes necroptosis or apoptosis. Induction of necroptosis, as a backup-programmed cell death pathway via ROS stimulation, offers a new strategy for the treatment of breast cancer.
Shikonin;ROS;necroptosis;apoptosis;breast cancer;
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Shikonin induces glioma cell necroptosis in vitro by ROS overproduction and promoting RIP1/RIP3 necrosome formation, Acta Pharmacologica Sinica, 2017, 38, 11, 1543  crossref(new windwow)
Baghestani AR, Shahmirzalou P, Zayeri F, et al (2015). Prognostic factors for survival in patients with breast cancer referred to omitted cancer research center in Iran. Asian Pac J Cancer Prev, 16, 5081-4. crossref(new window)

Christofferson DE LY, Yuan J (2014). Control of life-or-death decisions by RIP1 kinase. Annu Rev Physiol, 76, 129-50. crossref(new window)

Christofferson DE (2010). Necroptosis as an alternative form of programmed cell death. Curr Opin Cell Biol, 22, 263-8. crossref(new window)

Doval DC SA, Sinha R, Kumar K, et al (2015). Immunohistochemical profile of breast cancer patients at a tertiary care hospital in new delhi, India. Asian Pac J Cancer Prev, 16, 4959-64. crossref(new window)

Fu Z DB, Liao Y, Shan L, et al (2013). The anti-tumor effect of shikonin on osteosarcoma by inducing RIP1 and RIP3 dependent necroptosis. BMC Cancer, 13, 580. crossref(new window)

Ghavami S HM, Ande SR, Yeganeh B, et al (2009). Apoptosis and cancer: mutations within caspase genes. J Med Genet, 46, 497-510. crossref(new window)

Huang C LY, Zhao J, Yang F, et al (2013). Shikonin kills glioma cells through necroptosis mediated by RIP-1. PLoS One, 8, 66326. crossref(new window)

Jen-Tsung Yang Z-LL, Jin-Yi Wu, Fung-Jou Lu, Ching-Hsein Chen (2014). An oxidative stress mechanism of shikonin in human glioma cells. PLOS ONE, 9, 1-12.

Mansoori AA (2015). Molecular links between alcohol and tobacco induced dna damage, gene polymorphisms and patho-physiological consequences: a systematic review of hepatic carcinogenesis. Asian Pac J Cancer Prev, 16, 4803-12. crossref(new window)

Meka PB JS, Nanchari SR, Vishwakarma SK, et al (2015). LCN2 promoter methylation status as novel predictive marker for microvessel density and aggressive tumor phenotype in breast cancer patients. Asian Pac J Cancer Prev, 16, 4965-9. crossref(new window)

Park S, Cho Y (2013). Shikonin induces programmed necrosislike cell death through the formation of receptor interacting protein 1 and 3 complex. Food Chem Toxicol, 55, 36-41. crossref(new window)

Sadeghi RN, Karami-Tehrani F, Salami S (2015). Targeting prostate cancer cell metabolism: impact of hexokinase and CPT-1 enzymes. Tumour Biol, 36, 2893-905. crossref(new window)

Salami S, Karami-Tehrani F (2003). Biochemical studies of apoptosis induced by tamoxifen in estrogen receptor positive and negative breast cancer cell lines. Clin Biochem, 36, 247-53. crossref(new window)

Sharifian A PM, Emadedin M, Rostami Nejad M, et al (2015). Burden of breast cancer in iranian women is increasing, Asian Pac J Cancer Prev, 16, 5049-52. crossref(new window)

Shindo R, Okumura K, Kumagai Y, Nakano H (2013). Critical contribution of oxidative stress to TNFa-induced necroptosis downstream of RIPK1 activation. Biochem Biophys Res Commun, 436, 212-6. crossref(new window)

Siao AC, Hou CW, Kao YH, et al (2015). Effect of sesamin on apoptosis and cell cycle arrest in human breast cancer mcf-7 cells. Asian Pac J Cancer Prev, 16, 3779-83. crossref(new window)

Singha PK, Pandeswara S, Venkatachalam MA, et al (2013). Manumycin A inhibits triple-negative breast cancer growth through LC3-mediated cytoplasmic vacuolation death. Cell Death Dis, 4, 457. crossref(new window)

Stoscheck CM (1990). Quantitation of protein. Methods Enzymol, 182, 50-68. crossref(new window)

Tavakoli-Yaraki M, Karami-Tehrani F, Salimi V, et al (2013). Induction of apoptosis by Trichostatin A in human breast cancer cell lines: involvement of 15-Lox-1. Tumour Biol, 34, 241-9. crossref(new window)

Wang HY, Zhang B (2015). Cobalt chloride induces necroptosis in human colon cancer HT-29 cells. Asian Pac J Cancer Prev, 16, 2569-74. crossref(new window)

Wiench B ET, Paulsen M, Efferth T (2012). Shikonin directly targets mitochondria and causes mitochondrial dysfunction in cancer cells. Evid Based Complement Alternat Med, 2012, 726025.

Wu H XJ, Pan Q, Wang B, Hu D, Hu X (2013). Anticancer Agent Shikonin Is an Incompetent Inducer of Cancer Drug Resistance. PLoS One, 8, 52706. crossref(new window)

Xin Y, Li XY, Sun SR, et al (2015). Vegetable oil intake and breast cancer risk: a meta-analysis. Asian Pac J Cancer Prev, 16, 5125-35. crossref(new window)

Xun Hu WHLL (2007). Targeting the weak point of cancer by induction of necroptosis. Autophagy, 3, 490-2. crossref(new window)