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Radiosensitization Effects of a Zataria multiflora Extract on Human Glioblastoma Cells
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 Title & Authors
Radiosensitization Effects of a Zataria multiflora Extract on Human Glioblastoma Cells
Aghamohammadi, Azar; Hosseinimehr, Seyed Jalal; Ghasemi, Arash; Azadbakht, Mohammad; Pourfallah, Tayyeb Allahverdi;
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 Abstract
Background: Although radiotherapy is one of the most effective strategies in the treatment of cancers, it is associated with short and long term side effects on normal tissues. Zataria multiflora Boiss (Laminacea) (ZM) has several biological properties such as antioxidant and anti-inflammation activities.Here we investigated cell killing effects of a hydroalcoholic Zataria multiflora extract on cell death induced by ionizing radiation in a human glioblastoma cell line (A172) and human non-malignant fibroblasts (HFFF2) in vitro. Materials and Methods: A172 and HFFF2 cells were treated with a hydroalcoholic extract of dried aerial parts of Zataria multiflora at different concentrations (25, 50, 100, 150 and ) and then exposed to ionizing radiation (IR). Cell proliferation and DNA fragmentation were evaluated. Thymol content in the extract was analyzed and quantified by HPLC methods. Results: A172 cell proliferation was significantly inhibited by ZM. The percentage cell survival was for cells treated with of ZM extract alone while it was and for cells treated with ZM and exposed to IR at doses of 3Gy and 6Gy, respectively. Radiation-induced apoptosis in A172 cells was significantly increased following treatment with ZM at doses of . ZM extract did not exhibit any enhanced cell killing effects and apoptosis caused by IR on HFFF2 cells. Conclusions: These data show selective radiosensitization effects of ZM in A172 cells apparently due to increased radiation-induced apoptosis.
 Keywords
Ionizing radiation;Zataria multiflora;anti-proliferation;apoptosis;radiosensitive;
 Language
English
 Cited by
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Anticancer and Radiosensitization Efficacy of Nanocomposite Withania somnifera Extract in Mice Bearing Tumor Cells,;;;;;;;

Asian Pacific Journal of Cancer Prevention, 2016. vol.17. 9, pp.4367-4375 crossref(new window)
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Review of Natural Product-Derived Compounds as Potent Antiglioblastoma Drugs, BioMed Research International, 2017, 2017, 2314-6141, 1  crossref(new windwow)
2.
Pharmacological Properties and Molecular Mechanisms of Thymol: Prospects for Its Therapeutic Potential and Pharmaceutical Development, Frontiers in Pharmacology, 2017, 8, 1663-9812  crossref(new windwow)
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