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Techniques for Evaluation of LAMP Amplicons and their Applications in Molecular Biology
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 Title & Authors
Techniques for Evaluation of LAMP Amplicons and their Applications in Molecular Biology
Esmatabadi, Mohammad javad Dehghan; Bozorgmehr, Ali; zadeh, Hesam Motaleb; Bodaghabadi, Narges; Farhangi, Baharak; Babashah, Sadegh; Sadeghizadeh, Majid;
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 Abstract
Loop-mediated isothermal amplification (LAMP) developed by Notomi et al. (2000) has made it possible to amplify DNA with high specificity, efficiency and rapidity under isothermal conditions. The ultimate products of LAMP are stem-loop structures with several inverted repeats of the target sequence and cauliflower-like patterns with multiple loops shaped by annealing between every other inverted repeats of the amplified target in the similar strand. Because the amplification process in LAMP is achieved by using four to six distinct primers, it is expected to amplify the target region with high selectivity. However, evaluation of reaction accuracy or quantitative inspection make it necessary to append other procedures to scrutinize the amplified products. Hitherto, various techniques such as turbidity assessment in the reaction vessel, post-reaction agarose gel electrophoresis, use of intercalating fluorescent dyes, real-time turbidimetry, addition of cationic polymers to the reaction mixture, polyacrylamide gel-based microchambers, lateral flow dipsticks, fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays and nanoparticle-based colorimetric tests have been utilized for this purpose. In this paper, we reviewed the best-known techniques for evaluation of LAMP amplicons and their applications in molecular biology beside their advantages and deficiencies. Regarding the properties of each technique, the development of innovative prompt, cost-effective and precise molecular detection methods for application in the broad field of cancer research may be feasible.
 Keywords
LAMP;isothermal conditions;evaluation of reaction accuracy;quantitative inspections;various techniques;
 Language
English
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Peroxidyme-Amplified Radical Chain Reaction (PARCR): Visible Detection of a Catalytic Reporter, Angewandte Chemie International Edition, 2017, 56, 43, 13411  crossref(new windwow)
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 References
1.
Chou PH, Lin YC, Teng PH, et al (2011). Real-time targetspecific detection of loop-mediated isothermal amplification for white spot syndrome virus using fluorescence energy transfer-based probes. J Virol Methods, 173, 67-74. crossref(new window)

2.
Goto M, Honda E, Ogura A, et al (2009). Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques, 46, 167-72. crossref(new window)

3.
Lam L, Sakakihara S, Ishizuka K, et al (2008). Loop-mediated isothermal amplification of a single DNA molecule in polyacrylamide gel-based microchamber. Biomed Microdevices, 10, 539-46. crossref(new window)

4.
Mori Y, Hirano T, Notomi T (2006). Sequence specific visual detection of LAMP reactions by addition of cationic polymers. BMC Biotechnol, 6, 3. crossref(new window)

5.
Mori Y, Kitao M, Tomita N, et al (2004). Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods, 59, 145-57. crossref(new window)

6.
Mori Y, Nagamine K, Tomita N, et al (2001). Detection of loopmediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun, 289, 150-4. crossref(new window)

7.
Mori Y, Notomi T (2009). Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother, 15, 62-9. crossref(new window)

8.
Nakamura N, Ito K, Takahashi M, et al (2007). Detection of six single-nucleotide polymorphisms associated with rheumatoid arthritis by a loop-mediated isothermal amplification method and an electrochemical DNA chip. Anal Chem, 79, 9484-93. crossref(new window)

9.
Nimitphak T, Kiatpathomchai W, Flegel TW (2008). Shrimp hepatopancreatic parvovirus detection by combining loop-mediated isothermal amplification with a lateral flow dipstick. J Virol Methods, 154, 56-60. crossref(new window)

10.
Notomi T, Okayama H, Masubuchi H, et al (2000). Loopmediated isothermal amplification of DNA. Nucleic Acids Res, 28, 63. crossref(new window)

11.
Parida M, Horioke K, Ishida H, et al (2005). Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol, 43, 2895-903. crossref(new window)

12.
Ravan H, Yazdanparast R (2012). Development and evaluation of a loop-mediated isothermal amplification method in conjunction with an enzyme-linked immunosorbent assay for specific detection of Salmonella serogroup D. Anal Chim Acta, 733, 64-70. crossref(new window)

13.
Ravan H, Yazdanparast R (2013). Loop region-specific oligonucleotide probes for loop-mediated isothermal amplification-enzyme-linked immunosorbent assay truly minimize the instrument needed for detection process. Anal Biochem, 439, 102-8. crossref(new window)

14.
Seetang-Nun Y, Jaroenram W, Sriurairatana S, et al (2013). Visual detection of white spot syndrome virus using DNAfunctionalized gold nanoparticles as probes combined with loop-mediated isothermal amplification. Mol Cell Probes, 27, 71-9. crossref(new window)

15.
Soliman H, El-Matbouli M (2006). Reverse transcription loopmediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS). Vet Microbiol, 114, 205-13. crossref(new window)

16.
Tomita N, Mori Y, Kanda H, et al (2008). Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc, 3, 877-82. crossref(new window)