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Antitumoral and Antioxidant Potential of Egyptian Propolis Against the PC3 Prostate Cancer Cell Line
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 Title & Authors
Antitumoral and Antioxidant Potential of Egyptian Propolis Against the PC3 Prostate Cancer Cell Line
Salim, Elsayed I; Abd El-Magid, Afaf D; Farara, Khalid M; Maria, Dina SM;
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 Abstract
It has been shown previously that nutritional supplements rich in polyphenolic compounds play a significant role in prostate cancer chemoprevention. Propolis is a natural, resinous hive product that has several pharmacological activities including antimicrobial, antioxidant, anti-inflammatory, and antitumoral activities. The aim of this study was to compare the cytotoxic, antioxidant and antitumoral activities of an ethanolic extract of Egyptian propolis (EEP) in vitro with an established chemotherapeutic drug such as doxorubicin (DOX), and the effects of their combination against the PC3 human prostate cancer cell line. Cellular viability and levels with EEP, DOX and their (v/v) combination were detected by sulphorhodamine-B (SRB) assay after incubation of PC3 cells for 72h with different doses (0, 0.01, 0.1, 1, 10 and ). Two selected doses of and were applied to cells for 24h for antitumor evaluation assay of treatment compounds. EEP and its (v/v) combination with DOX showed significant antitumor potential besides high antioxidant properties of superoxide dismutase (SOD), total antioxidant capacity (TAC), catalase (CAT), nitric oxide (NO) and reduced glutathione (GSH) levels when compared with the control untreated cells. DNA fragmentation assay and semi quantitative RT-PCR analyses for p53 and Bax genes showed that EEP activated cellular apoptosis and increased the mRNA expression levels more than other treatment. In conclusion, EEP alone or in combination with DOX at both doses used here showed greater antioxidant, antiproliferative and apoptotic effects against the PC3 cell lines as compared to treatment with DOX alone. Therefore, EEP could be considered as a promising candidate for prostate cancer chemotherapy.
 Keywords
Propolis;prostate cancer;PC3 cell line;p53;Bax;oxidative stress;apoptosis;
 Language
English
 Cited by
1.
Anti-proliferative and anti-migration effects of Polish propolis combined with Hypericum perforatum L. on glioblastoma multiforme cell line U87MG, BMC Complementary and Alternative Medicine, 2016, 16, 1  crossref(new windwow)
 References
1.
Abd El-Hady FK, Hegazi AG (1994). Gas chromatography -mass spectrometry (GC/MS) study of the Egyptian propolis 1-aliphatic, phenolic acids and their esters. Egypt J Appl Sci, 9, 749-60.

2.
Abozid MM, Ahmed AA (2013). Chemical composition of Egyptian and commercial propolis and its effects on liver function and lipid profiles in albino rats. J Biological Chem. Env Res, 8, 323-40.

3.
Adams JM, Cory S (1998). The bcl-2 protein family, Arbiters of cell survival. Science, 281, 1322-6. crossref(new window)

4.
Akyol S, Ugurcu V, Balci M, et al (2014). Caffeic acid phenethyl ester, its protective role against certain major eye diseases. J Ocul Pharmacol Ther, 30, 700-8. crossref(new window)

5.
Alyane M, Kebsa LB, Boussenane H, et al (2008). Cardioprtective effects and mechanism of action of poly phenols extracted from propolis against doxorubicin toxicity. Pak J pharm Sci, 1, 20-9.

6.
Aso K, Kanno S, Tadano T, et al (2004). Inhibitory effect of propolis on the growth of human leukemic U937. Biol Pharm Bull, 27, 727-30. crossref(new window)

7.
Atsushi K, Mutsuo F, Manabu M, et al (2001). Expression of Bax and apoptosis-related proteins in human esophageal squamous cell carcinoma including dysplasia. Canadian Academy Pathol, 14, 741-7.

8.
Baillie JK, Thompson AAR, Irving JB, et al (2009). Oral antioxidant supplementation does not prevent acute mountain sickness, double blind randomized placebocontrolled trial. QJM, Int J Med, 102, 341-8. crossref(new window)

9.
Begnini KR, Moura de Leon PM, Thurow H, et al (2014). Brazilian red propolis induces apoptosis-like cell death and decreases migration potential in bladder cancer cells. Evid Based Complement Alternat Med, 2014, 639856.

10.
Beutler E, Dunning D, Dabe IB, et al (1988). Erythrocyte glutathione S-transferase deficiency and hemolytic anemia. Blood, 72, 73-7.

11.
Chaabane W, User SD, El-Gazzah M, et al (2013). Autophagy, apoptosis, mitoptosis and necrosis, interdependence between those pathways and effects on cancer. Arch Immunol Ther Exp (Warsz), 61, 43-58. crossref(new window)

12.
Chan GC, Cheung KW, Sze DM. (2013). The immunomodulatory and anticancer properties of propolis. Clin Rev Allergy Immunol, 44, 262-73. crossref(new window)

13.
Chen CN, Wu CL, Lin JK (2007). Apoptosis of human melanoma cells induced by the novel compounds propolin A and propolin B from Taiwenese propolis. Cancer Lett, 245, 218-31. crossref(new window)

14.
Chen MJ, Chang WH, Lin CC, et al (2008). Caffeic acid phenethyl ester induces apoptosis of human pancreatic cancer cells involving caspase and mitochondrial dysfunction. Pancreatol, 8, 566-76. crossref(new window)

15.
Christine ME, Martine FR, Stanley JK, et al (2001). Bax loss impairs myc-induced apoptosis and circumvents the selection of p53 mutations during myc-mediated lymphomagenesis. Mol Cell Biol, 21, 7653-62. crossref(new window)

16.
Daugsch A, Moraes CS, Fort P, et al (2008). Brazilian red propolis chemical composition and botanical origin. Evid Based Comp Alternat Med, 5, 435-41. crossref(new window)

17.
Goel M, Eraslan G, Silici S (2009). Antioxidant effect of propolis against exposure to propetamphos in rats. Ecotoxicol. Environ Safety, 72, 900-915.

18.
Harris SR, Panaro NJ, Thorgeirsson UP (2000). Oxidative stress contributes to the anti-proliferative effects of flavone acetic acid on endothelial cells. Anticancer Res, 20, 2249-54.

19.
Hilger RA, Richly H, Grubert M, et al (2005). Pharmacokinetics (PK) of a liposomal encapsulated fraction containing doxorubicin and of doxorubicin released from the liposomal capsule after intravenous infusion of Caelyx/Doxil. Int J Clin Pharmacol Ther, 43, 588. crossref(new window)

20.
Janicke RU, Sohn D, Schulze-Osthoff K (2008). The dark side of a tumor suppressor, anti-apoptotic p53. Cell Death Differ, 15, 959-76. crossref(new window)

21.
Jasprica I, Mornar A, Debeljak Z, et al (2007). In vivo study of propolis supplementation effects on antioxidative status and red blood cells. J Ethnopharmacol, 110, 548-54. crossref(new window)

22.
Kasala ER, Bodduluru LN, Madana RM, et al (2015). Chemopreventive and therapeutic potential of chrysin in cancer, mechanistic perspectives. Toxicol Lett, 233, 214-25. crossref(new window)

23.
Khalil ML (2006). Biological activity of bee propolis in health and disease, Asian Pac J Cancer Prev, 7, 22-31.

24.
Kimoto T, Arai S, Kohguchi M, et al (1998). Apoptosis and suppression of tumor growthby artepillin C extracted from Brazilian propolis. Cancer Detect Prev, 22, 506-15. crossref(new window)

25.
Kimoto T, Aga M, Hino K, et al (2001). Apoptosis of human leukemia cells induced by Artepillin C, an active ingredient of Brazilian propolis. Anticancer Res, 21, 221-8.

26.
Kujumgiev A, Tsvetkova I, Serkedjieva Y, et al (1999). Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol, 64, 235-240. crossref(new window)

27.
Kumazawa S, Hamasaka T, Nakayama T. (2004). Antioxidant activity of propolis of various geographic origins. Food Chem, 84, 329-39. crossref(new window)

28.
Leist M, Jaattela M (2001). Four deaths and a funeral, from caspases to alternative mechanisms. Nature Rev Molecular Cell Biol, 2, 589-98.

29.
Marghitas LA, Laslo L, Dezmirean D, et al (2007). Total phenolics and antioxidant activity of romanian propolis. international conference agricultural and food sciences, progresses and technologies, sibiu, romania, 209-12.

30.
Marghitas AL, Dezmirean D, Moise A, et al (2009). DPPH method for evaluation of propolis antioxidant activity. Bulletin UASVM Animal Sci Biotechnol, 66, 253-8.

31.
Markiewicz-Ukowska R, Borawska MH, Fiedorowicz A, et al (2013). Propolis changes the anticancer activity of temozolomide in U87MG human glioblastoma cell line. BMCComplement Altern Med, 13, 50. crossref(new window)

32.
Mishima S, Narita Y, Chikamatsu S (2005). Effects of propolis on cell growth and gene expression in HL-60 cells. J Ethnopharmacol, 99, 5-11. crossref(new window)

33.
Montgomery JF, Hum S (1995). Field diagnosis of nitrite poisoning in cattle by testing aqueous humour samples with urine test strips. Vet Rec, 137, 593-4.

34.
Moreira L, Dias LG, Pereira JA, et al (2008). Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chem Toxicol, 46, 3482-85. crossref(new window)

35.
Motomura MK, Won KM, Suh SJ, et al (2008). Propolis induces cell cycle arrest and apoptosis in human leukemic U937 cells through Bcl-2/Bax regulation. Environ Toxicol Pharmacol, 26, 61-7. crossref(new window)

36.
Murtaza G, Karim S, Akram MR, et al (2014). Caffeic acid phenethyl ester and therapeutic potentials. Biomed Res Int, 145342, 9.

37.
Orsatti CL, Missima F, Pagliarone AC, et al (2010). Propolis immunomodulatory action in vivo on Toll-like receptors 2 and 4 expression and on pro-inflammatory cytokines production in mice. Phytotherapy Res, 24, 1141-6.

38.
Orsolic N, Horvatknezevic A, Benkovic V, et al (2008). Benefits of use of propolis and related flavonoids against the toxicity of chemotherapeutic agents. in orsolic, n; basic, i (eds). ethnopharmacology- review book. Res Signpost India, 195-222.

39.
Orsolic N (2010). A review of propolis antitumour action in vivo and in vitro. J Api Productand Ap Med Sci, 2, 1-20. crossref(new window)

40.
Quinn M, Babb P (2002). Patterns and trends in prostate cancer incidence, survival, prevalence and mortality. Part I, International comparisons. BJU Int, 90, 162-73. crossref(new window)

41.
Sabir SM, Ahmad SD, Abdul H, et al (2012). Antioxidant and Hepatoprotective activity of ethanolic extract of leaves of Solidago microglossa containing polyphenolic compounds. Food Chem, 131, 741-7. crossref(new window)

42.
Salim EI, Jazieh AR, Moore MA (2011). Lung cancer incidence in the Arab league countries, risk factors and control. Asian Pac J Cancer Prev, 12, 17-34.

43.
Samarghandian S, Shabestari MM (2013). DNA fragmentation and apoptosis induced by safranal in human prostate cancer cell line. Indian J Urol 29, 177-83 crossref(new window)

44.
Samarghandian S, Borji A, Delkhosh MB, et al. (2013). Safranal treatment improves hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced diabetic rats. J Pharm Pharm Sci, 16, 352-62.

45.
Samini F, Samarghandian S, Borji A, et al (2013). Curcumin pretreatment attenuates brain lesion size and improves neurological function following traumatic brain injury in the rat. Pharmacol Biochem Behav, 110, 238-44. crossref(new window)

46.
Scheller S, Krol W, Swiacik J, et al (1989). Antitumoral property of ethanolic extract of propolis in mice-bearing Ehrlich carcinoma, as compared to bleomycin. Z Naturforsch C, 44, 1063-5.

47.
Scifo C, Cardile V, Russo A, et al (2004). Resveratrol and propolis as necrosis or apoptosisinducers in human prostate carcinoma cells. Oncol Res, 14, 415-26.

48.
Shiraishi N, Inai Y, Bi W, Nishikimi M (2005). Fragmentation and dimerization of copper-loaded prion protein by coppercatalysed oxidation. Biochem J, 387, 247-55. crossref(new window)

49.
Siddhuraju P (2006). The antioxidant activity and free radicalscavenging capacity of phenolics of raw and dry heated moth bean. (Vigna aconitifolia) (Jacq.) Marcchal seed extracts, 99, 149-57. crossref(new window)

50.
Sinha BK, Trush MA, Kennedt KA, et al (1984). Enzymatic activation and binding of adriamycin to nuclear DNA. Cancer Res, 44, 2892.

51.
Skehan P, Storeng R, Scudiero D, et al (1990). New colorimetric cytotoxicity assay for anticancer-drug screening. J Nat Cancer Instit, 82, 1107-12. crossref(new window)

52.
Sujoy B, Ramesh MR, Leonard RJ (2009). Role of polyamines in p53-dependent apoptosis of intestinal epithelial cells. Cellular Signalling, 21, 509-22. crossref(new window)

53.
Szliszka E, Czuba ZP, Domino M, et al (2009). Ethanolic extract of propolis (EEP) enhances the apoptosis-inducing potential of TRAIL in cancer cells. Molecules, 14, 738-54. crossref(new window)

54.
Szliszka E, Czuba ZP, Bronikowska J, et al (2011). Ethanolic extract of propolis augments TRAIL-induced apoptotic death in prostate cancer cells. Evid Based Complement Alternat Med, 2011, 535172

55.
Takashi S (2009). Zinc-finger-based artificial transcription factors and their applications. Advanced Drug Delivery Reviews, 43, 8510-615.

56.
Taraswi B, Somsubhra N, Susanta R (2009). DNA damage induced p53 down regulates Cdc20 by direct binding to its promoter causing chromatin remodeling. Nucleic Acids Res, 10, 1-11.

57.
Teixeira EW, Message D, Negri G, et al (2010). Seasonal variation, chemical composition and antioxidant activity of Brazilian propolis samples. Evid Based Comp Alter Med, 7, 307-15. crossref(new window)

58.
Yong YK, Tan JJ, The SS, et al (2013). Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines. Evid Based Complement Alternat Med, 2013, 462751

59.
Youle RJ, Strasser A (2008). The BCL-2 protein family, opposing activities that mediate cell death. Nature Reviews Mol Cell Biol, 9, 47-59. crossref(new window)

60.
Zand RS, Jenkins DJ, Diamandis EP (2000). Steroid hormone activity of flavonoids and related compounds. Breast Cancer Res Treat, 62, 35-49. crossref(new window)

61.
Valente MJ, Baltazar AF, Henrique R, et al (2011). Biological activities of portuguese propolis, protection against free radical-induced erythrocyte damage and inhibition of human renal cancer cell growth in vitro. Food Chem. Toxicol, 49, 86-92. crossref(new window)

62.
Vatansever HS, Sorkun K, Gurhan SID (2010). Propolis from Turkey induces apoptosis through activating caspases inhuman breast carcinoma cell lines. Acta Histochemica, 112, 546-56. crossref(new window)

63.
Vogelstein B, Lane D, Levine AJ (2000). Surfing the p53 network. Nature, 408, 307-10. crossref(new window)

64.
Yagmurca M, Erdogan H, Iraz M, et al (2004). Caffeic acid phenethyl ester as a protective agent against doxorubicin nephrotoxicity in rats. Clin Chim Acta, 348, 27-34. crossref(new window)

65.
Yasuo T, Nobuyuki A, Shinsuke I, et al (2004). Loss of caspase-8 activation pathway is a possible mechanism for CDDP resistance in human laryngeal squamous cell carcinoma HEP-2 cells. International J Oncol, 25, 721-8.