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DNA Ploidy and S-phase Fraction Analysis in Paediatric B-cell Acute Lymphoblastic Leukemia Cases: a Tertiary Care Centre Experience
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 Title & Authors
DNA Ploidy and S-phase Fraction Analysis in Paediatric B-cell Acute Lymphoblastic Leukemia Cases: a Tertiary Care Centre Experience
Kumar, Banothu Kiran; Bhatia, Prateek; Trehan, Amita; Singh, Ajit Pal; Kaul, Deepak; Bansal, Deepak;
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 Abstract
DNA ploidy is an important prognostic parameter in paediatric B-ALL, but the significance of the S-phase fraction is unclear. In present study, DNA ploidy was assessed in 40 pediatric B-ALL cases by flow cytometry. The DI (DNA index) and percentage of cells in S-phase were calculated using Modfit software. Aneuploidy was noted in 26/40 (65%) cases. A DI of 1.10-1.6 (hyperdiploidy B) was noted in 20/40 (50%) and 6/40 (15%) had a DI>1.60 (triploid and tetraploid range). Some 14/40 (35%) cases had a diploid DI between 0.90-1.05. None of the cases had a DI <0.90 (hypodiploid) or in the 1.06-1.09 (hyperdiploid A) range. The mean S-phase fraction was 2.6%, with 24/40 (60%) having low and 16/40 (40%) high S-phase fractions. No correlation was noted with standard ALL risk and treatment response factors with DI values or S-phase data, except for a positive correlation of low S-phase with high NCI risk category (p
 Keywords
ALL;DNA-index;ploidy;S-phase fraction;paediatric leukemia cases;
 Language
English
 Cited by
1.
Simultaneous use of multiplex ligation-dependent probe amplification assay and flow cytometric DNA ploidy analysis in patients with acute leukemia, Cytometry Part B: Clinical Cytometry, 2017, 15524949  crossref(new windwow)
 References
1.
Amare P, Gladstone B, Varghesse C, Pai S, Advani S (1999). Clinical significance of cytogenetic findings at diagnosis and in remission in childhood and adult Acute Lymphoblastic leukemia: Experience from India. Cancer Genet Cytogenet, 110, 44-53. crossref(new window)

2.
Basu O, Zolzer F, Uma DP (2009). DNA Ploidy - A Prognostic Factor of Acute Lymphoblastic Leukemia (ALL) in Childhood. Asian J Exp Sci, 23, 33-8.

3.
Faderl S, Kantarjian HM, Talpaz M, Estrov Z (1998). Clinical significance of cytogenetic abnormalities in adult acute lymphoblastic leukemia. Blood, 91, 3995-4019.

4.
Foud M AT, Maha R AW, Hala A AA, Amal A Z, Saed S E (2002). Flow cytometric assessment of nuclear DNA ploidy and proliferative activity in acute lymphoblastic leukemia. J Nat Egyptian Cancer Inst, 14, 303-10.

5.
Hilden JM, Dinndorf PA, Meerbaum SO, et al (2006). Children's Oncology Group. Analysis of prognostic factors of acute lymphoblastic leukemia in infants: report on CCG 1953 from the Children's Oncology Group. Blood, 108, 441-51. crossref(new window)

6.
Jena RK, Patnaik SC, Sahu GR, Ray B, Swain K (2002). Secondary chromosomal abnormalities in Acute lymphoblastic Leukemia. Caryologia, 55, 349-55. crossref(new window)

7.
Kaaijk P, Kaspers GJL, Van-Wering ER, Broekema GJ, Loonen AH, Hahlen K (2003). Cell proliferation is related to in vitro drug resistance in childhood acute leukaemia. Br J Cancer. 88, 775-81. crossref(new window)

8.
Khalifa AS, El-Kardani TA, Faeyk MH (1997). Flowcytometric study childhood acute leukemia immunophenotype and DNA analysis. Egypt J Haematol, 22, 1-23.

9.
Lustosa de Sousa DW, de Almeida Ferreira FV, Cavalcante Felix FH, de Oliveira Lopes MV (2015). Acute lymphoblastic leukemia in children and adolescents: prognostic factors and analysis of survival. Rev Bras Hematol Hemoter. 37, 223-9. crossref(new window)

10.
Mullighan CG, Su X, Zhang J, et al (2009). Children's Oncology Group. Deletion of IKZF1 and prognosis in acute lymphoblastic leukemia. N Engl J Med, 360, 470-80. crossref(new window)

11.
N Chaudhary, A Borker (2013). Significance of karyotyping in childhood acute lymphoblastic leukemia. Pediatric Oncall Journal, 10, 39-41.

12.
Olah E, Balogh E, Pajor L, Jakab Z (2011). Ten-year experiences on initial genetic examination in Childhood Acute Lymphoblastic Leukemia in Hungary (1993-2002): Technical Approaches and Clinical Implementation. Pathol Oncol Res, 17, 81-90. crossref(new window)

13.
Raimondi SC, Behm FG, Roberson PK, et al (1988). Cytogenetics of childhood T-cell leukemia. Blood, 72, 1560-6.

14.
Raimondi SC, Zhou Y, Mathew S, et al (2003). Reassessment of the prognostic significance of hypodiploidy in pediatric patients with acute lymphoblastic leukemia. Cancer, 98, 2715-22. crossref(new window)

15.
Shaikh MS, Ali SS, Khurshid M, Fadoo Z (2014). Chromosomal abnormalities in Pakistani children with acute lymphoblastic leukemia. Asia Pac J Cancer Prev, 15, 3907-9. crossref(new window)

16.
Somnath P, Rajlaxmi S, Pranati M, Rupa Das, Sukumar C, Raghumani M (2011). Cytogenetic profile of pediatric acute lymphoblastic leukemia (ALL): Analysis of 31 cases with review of literature. Caryologia, 64, 33-41. crossref(new window)

17.
Woo JS, Alberti MO, Tirado CA (2014). Childhood B-acute lymphoblastic leukemia: a genetic update. Experimental Hematology & Oncology, 3, 1-14. crossref(new window)

18.
XM Wang (2014). Advances and issues in flow cytometric detection of immunophenotypic changes and genomic rearrangements in acute pediatric leukemia. Transl Pediatr, 3, 149-55.

19.
Yeoh EJ, Ross ME, Shurtleff SA, et al (2002). Classification, subtype discovery and prediction of outcome in pediatric acute lymphoblastic leukemia by gene expression profiling. Cancer Cell, 1, 133-43. crossref(new window)