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Upregulation of Mir-34a in AGS Gastric Cancer Cells by a PLGA-PEG-PLGA Chrysin Nano Formulation
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 Title & Authors
Upregulation of Mir-34a in AGS Gastric Cancer Cells by a PLGA-PEG-PLGA Chrysin Nano Formulation
Mohammadian, Farideh; Abhari, Alireza; Dariushnejad, Hassan; Zarghami, Faraz; Nikanfar, Alireza; Pilehvar-Soltanahmadi, Yones; Zarghami, Nosratollah;
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Background: Nano-therapy has the potential to revolutionize cancer therapy. Chrysin, a natural flavonoid, was recently recognized as having important biological roles in chemical defenses and nitrogen fixation, with anti-inflammatory and anti-oxidant effects but the poor water solubility of flavonoids limitstheir bioavailability and biomedical applications. Objective: Chrysin loaded PLGA-PEG-PLGA was assessed for improvement of solubility, drug tolerance and adverse effects and accumulation in a gastric cancer cell line (AGS). Materials and Methods: Chrysin loaded PLGA-PEG copolymers were prepared using the double emulsion method (W/O/W). The morphology and size distributions of the prepared PLGA-PEG nanospheres were investigated by 1H NMR, FT-IR and SEM. The in vitro cytotoxicity of pure and nano-chrysin was tested by MTT assay and miR-34a was measured by real-time PCR. Results: 1H NMR, FT-IR and SEM confirmed the PLGA-PEG structure and chrysin loaded on nanoparticles. The MTT results for different concentrations of chrysin at different times for the treatment of AGS cell line showed IC50 values of 68.2, 56.2 and and 58.2, 44.2, after 24, 48, and 72 hours of treatment, respectively for chrysin itslef and chrysin-loaded nanoparticles. The results of real time PCR showed that expression of miR-34a was upregulated to a greater extent via nano chrysin rather than free chrysin. Conclusions: Our study demonstrates chrysin loaded PLGA-PEG promises a natural and efficient system for anticancer drug delivery to fight gastric cancer.
Chrysin;PLGA-PEG;MTT assay;gastric cancer;miR-34a;
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Alimohammadi YH, Joo SW (2014). PLGA-based nanoparticles as cancer drug delivery systems. Asian Pac J Cancer Prev, 15, 517-35. crossref(new window)

Basmadjian C, Zhao Q, Bentouhami E, et al (2014). Cancer wars: natural products strike back. Frontiers in chemistry, 2.

Chen S, Pieper R, Webster DC, et al (2005). Triblock copolymers: synthesis, characterization, and delivery of a model protein. International journal of pharmaceutics, 288, 207-18. crossref(new window)

Dwivedi M, Sharma S, Shukla P, et al (2014). Development and Evaluation of Anticancer Polymeric Nano-Formulations Containing Curcumin and Natural Bioenhancers. Journal of Biomaterials and Tissue Engineering, 4, 198-202. crossref(new window)

Fock K (2014). Review article: the epidemiology and prevention of gastric cancer. Alimentary pharmacology & therapeutics, 40, 250-60. crossref(new window)

Ghalhar MG, Akbarzadeh A, Rahmati M, et al (2013). Comparison of Inhibitory Effects of 17-AAG Nanoparticles and Free 17-AAG on HSP90 Gene Expression in Breast Cancer. Asian Pacific journal of cancer prevention: APJCP, 15, 7113-8.

Hu C-E, Liu Y-C, Zhang H-D, et al (2014). The RNA-binding protein PCBP2 facilitates gastric carcinoma growth by targeting miR-34a. Biochemical and biophysical research communications, 448, 437-42. crossref(new window)

Hur K, Toiyama Y, Takahashi M, et al (2013). MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis. Gut, 62, 1315-26. crossref(new window)

Ji Q, Hao X, Meng Y, et al (2008). Restoration of tumor suppressor miR-34 inhibits human p53-mutant gastric cancer tumorspheres. BMC cancer, 8, 266. crossref(new window)

Kasala ER, Bodduluru LN, Madana RM, et al (2015). Chemopreventive and therapeutic potential of chrysin in cancer: mechanistic perspectives. Toxicology Letters, 233, 214-25. crossref(new window)

Khoo BY, Chua SL, Balaram P (2010). Apoptotic effects of chrysin in human cancer cell lines. International J Molecular Sci, 11, 2188-99. crossref(new window)

Kwon Y, Kim H, Park S, et al (2010). Enhancement of solubility and antioxidant activity of some flavonoids based on the inclusion complexation with sulfobutylether ${\beta}$-cyclodextrin. Bull Korean Chem Soc, 31, 3035-7. crossref(new window)

Lee HJ, Song I-C, Yun H-J, et al (2013). CXC chemokines and chemokine receptors in gastric cancer: From basic findings towards therapeutic.

Munin A, Edwards-Levy F (2011). Encapsulation of natural polyphenolic compounds; a review. Pharmaceutics, 3, 793-829. crossref(new window)

Nejati-Koshki K, Akbarzadeh A, Pourhasan-Moghadam M, et al (2013). Inhibition of leptin and leptin receptor gene expression by silibinin-curcumin combination. Asian Pac J Cancer Prev, 14, 6595-9. crossref(new window)

Okuchukwu EH, Olayiwola OA (2015). Epidemiology and clinicopathological characteristics of gastric cancer--the Nigerian setting in view. Niger J Med, 24, 71-80.

Samarghandian S, Afshari JT, Davoodi S (2011). Chrysin reduces proliferation and induces apoptosis in the human prostate cancer cell line pc-3. Clinics, 66, 1073-9. crossref(new window)

Wang Q-X, Zhu Y-Q, Zhang H, et al (2015). Altered MiRNA Expression in Gastric Cancer: a Systematic Review and Meta-Analysis. Cellular Physiology and Biochemistry, 35, 933-44. crossref(new window)

Zarouni M, Salehi R, Akbarzadeh A, et al (2015). Biocompatible Polymer Coated Paramagnetic Nanoparticles for Doxorubicin Delivery: Synthesis and Anticancer Effects Against Human Breast Cancer Cells. International Journal of Polymeric Materials and Polymeric Biomaterials, 64, 718-26. crossref(new window)

Zhang T, Chen X, Qu L, et al (2004). Chrysin and its phosphate ester inhibit cell proliferation and induce apoptosis in Hela cells. Bioorganic & medicinal chemistry, 12, 6097-105. crossref(new window)