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Effect of First Line Gastric Cancer Chemotherapy Regime on the AGS Cell Line - MTT Assay Results
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 Title & Authors
Effect of First Line Gastric Cancer Chemotherapy Regime on the AGS Cell Line - MTT Assay Results
Alizadeh-Navaei, Reza; Rafiei, Alireza; Abedian-Kenari, Saeid; Asgarian-Omran, Hossein; Valadan, Reza; Hedayatizadeh-Omran, Akbar;
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Background: Combination chemotherapy regimes are common treatments for cancer. The aim of this study was to evaluation the effect of individual chemotherapeutic agents in comparison with a first line chemotherapy regime treatment in the AGS gastric cancer cell line by MTT assay. Materials and Methods: In this experimental study, AGS cells were grown in RPMI-1640 supplemented with 10% fetal calf serum and 100 IU/ml penicillin, and streptomycinin, under a humidified condition at with 5% CO2. All cells were washed with PBS and detached with trypsin, centrifuged and 8000 cells re-plated on to 96- well plates. LD50 doses of Epirubicin, Cisplatin and 5-fluorouracil were added to each well in mono or triple therapy. Anti-proliferative activities were determined by MTT assay after 24, 48 or 72 h. Results: Results of MTT assays showed that there were no significant differences among 3 drugs in monotherapy (p
Gastric cancer;chemotherapy;AGS cell line;MTT;monotherapy;combined therapy;
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