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Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma
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 Title & Authors
Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma
Khor, Goot Heah; Froemming, Gabrielle Ruth Anisah; Zain, Rosnah Binti; Abraham, Thomas Mannil; Lin, Thong Kwai;
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 Abstract
Background: Promoter hypermethylation is a frequent epigenetic mechanism for gene transcription repression in cancer and is one of the hallmarks of the disease. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) contributes to cell contact-mediated communication. Dysregulation of promoter methylation has been reported in various cancers. Objectives: The objectives of this study were to investigate the CELSR3 hypermethylation level in oral squamous cell carcinomas (OSCCs) using methylation-sensitive high-resolution melting analysis (MS-HRM) and to correlate CELSR3 methylation with patient demographic and clinicopathological parameters. Materials and Methods: Frozen tissue samples of healthy subjects` normal mucosa and OSCCs were examined with regard to their methylation levels of the CELSR3 gene using MS-HRM. Results: MS-HRM analysis revealed a high methylation level of CELSR3 in 86% of OSCC cases. Significant correlations were found between CELSR3 quantitative methylation levels with patient ethnicity (P
 Keywords
CELSR3;hypermethylation;oral squamous cell carcinoma;biomarker;
 Language
English
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