Advanced SearchSearch Tips
Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma
Khor, Goot Heah; Froemming, Gabrielle Ruth Anisah; Zain, Rosnah Binti; Abraham, Thomas Mannil; Lin, Thong Kwai;
  PDF(new window)
Background: Promoter hypermethylation is a frequent epigenetic mechanism for gene transcription repression in cancer and is one of the hallmarks of the disease. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) contributes to cell contact-mediated communication. Dysregulation of promoter methylation has been reported in various cancers. Objectives: The objectives of this study were to investigate the CELSR3 hypermethylation level in oral squamous cell carcinomas (OSCCs) using methylation-sensitive high-resolution melting analysis (MS-HRM) and to correlate CELSR3 methylation with patient demographic and clinicopathological parameters. Materials and Methods: Frozen tissue samples of healthy subjects` normal mucosa and OSCCs were examined with regard to their methylation levels of the CELSR3 gene using MS-HRM. Results: MS-HRM analysis revealed a high methylation level of CELSR3 in 86% of OSCC cases. Significant correlations were found between CELSR3 quantitative methylation levels with patient ethnicity (P
CELSR3;hypermethylation;oral squamous cell carcinoma;biomarker;
 Cited by
MicroRNA-34a/EGFR axis plays pivotal roles in lung tumorigenesis, Oncogenesis, 2017, 6, 8, e372  crossref(new windwow)
MiRNA expression profiles reveal the involvement of miR-26a, miR-548l and miR-34a in hepatocellular carcinoma progression through regulation of ST3GAL5, Laboratory Investigation, 2017, 97, 5, 530  crossref(new windwow)
Amornpisutt R, Sriraksa R, Limpaiboon T (2012). Validation of methylation-sensitive high resolution melting for the detection of DNA methylation in cholangiocarcinoma. Clin Biochem, 45, 1092-4. crossref(new window)

Baylin SB, Ohm JE (2006). Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction? Nature Rev Cancer, 6, 107-16. crossref(new window)

Cianfrocca M, Goldstein LJ (2004). Prognostic and predictive factors in early-stage breast cancer. The Oncologist, 9, 606-16. crossref(new window)

Dobrowolski SF, Wittwer CT (2011). High-resolution melt profiling, in molecular analysis and genome discovery. John Wiley & Sons, Ltd, p. 81-113.

Einav Nili GY, Saito Y, Egger G, et al (2008). Cancer epigenetics, modifications, screening, and therapy. Annu Rev Med, 59, 267-80. crossref(new window)

Erkan M, Weis N, Pan Z, et al (2010). Organ-, inflammation-and cancer specific transcriptional fingerprints of pancreatic and hepatic stellate cells. Mol Cancer, 9, 88-103. crossref(new window)

Feng, J, Han Q, Zhou L (2012). Planar cell polarity genes, Celsr1-3 in neural development. Neurosci Bulletin, 28, 39-15. crossref(new window)

Herman JG, Baylin SB (2003). Gene silencing in cancer in association with promoter hypermethylation. New Engl J Med, 349, 2042-54. crossref(new window)

Jemal A, Siegel R, Ward E (2007). Ca_statistica. CA Cancer J Clin, 57, 43-66. crossref(new window)

Jeong P, Ha YS, Cho IC, et al (2011). Three-gene signature predicts disease progression of non-muscle invasive bladder cancer. Oncol Lett, 2, 679-84. crossref(new window)

Katoh M (2005). WNT/PCP signaling pathway and human cancer (Review). Oncol Reports, 14, 1583-8.

Katoh M, Katoh M (2007). Comparative integromics on noncanonical WNT or planar cell polarity signaling molecules, Transcriptional mechanism of PTK7 in colorectal cancer and that of SEMA6A in undifferentiated ES cells. Inter J Mol Med, 20, 405-9.

Khor GH, Froemming GRA, Zain RB, et al (2013). DNA methylation profiling revealed promoter hypermethylationinduced silencing of p16, DDAH2 and DUSP1 in primary oral squamous cell carcinoma. Inter J Med Sci, 10, 1727-39. crossref(new window)

Khor GH, Froemming GRA, Zain RB, et al (2014). Screening of differential promoter hypermethylated genes in primary oral squamous cell carcinoma. Asian Pacific J Cancer Prev, 15, 8957-61. crossref(new window)

Mascolo M, Siano M, Ilardi G, et al (2012). Epigenetic disregulation in oral cancer. Int J Mol Sc, 13, 2331-53. crossref(new window)

Montgomery JL, Sanford LN, Wittwer CT (2010). Highresolution DNA melting analysis in clinical research and diagnostics. Expert Rev Mol Diagn, 10, 219-40. crossref(new window)

Morris MR, Ricketts CJ, Gentle D, et a (2010). Genome-wide methylation analysis identifies epigenetically inactivated candidate tumour suppressor genes in renal cell carcinoma. Oncogene, 30, 1390-401.

Pimenta Amaral TM, da Silva Freire AR, Carvalho AL, et al (2004). Predictive factors of occult metastasis and prognosis of clinical stages I and II squamous cell carcinoma of the tongue and floor of the mouth. Oral Oncol, 40, 780-6. crossref(new window)

Reed GH, Kent JO, Wittwer CT (2007). High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics, 8, 597-608. crossref(new window)

Shen J, Wang S, Zhang YJ, et al (2012). Genome wide DNA methylation profiles in hepatocellular carcinoma. Hepatology, 55, 1799-808. crossref(new window)

Tian XY, Zhang L, Sun LG, et al (2015). Epigenetic Regulation of miR-129-2 Leads to Overexpression of PDGFRa and FoxP1 in Glioma Cells. Asian Pac J Cancer Prev, 16, 6129-33. crossref(new window)

Tissir F, Bar I, Jossin Y, et al (2005). Protocadherin Celsr3 is crucial in axonal tract development. Nature Neurosci, 8, 451-7. crossref(new window)

Wittwer CT (2009). High-resolution DNA melting analysis. advancements and limitations. Hum Mutat, 30, 857-9. crossref(new window)

Wojdacz TK, Dobrovic A (2007). Methylation-sensitive high resolution melting (MS-HRM), a new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res, 35, 41. crossref(new window)

Wojdacz TK, Dobrovic A, Hansen LL (2008). Methylationsensitive high-resolution melting. Nature Protoc, 3, 1903-8. crossref(new window)

Wojdacz TK, Borgbo T, Hansen LL (2009). Primer design versus PCR bias in methylation independent PCR amplifications. Epigenetics, 4, 231-4. crossref(new window)

Wojdacz TK, Moller TH, Thestrup BB, et al (2010). Limitations and advantages of MS-HRM and bisulfite sequencing for single locus methylation studies. Expert Rev Mol Diagn, 10, 575-80. crossref(new window)