JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Protection against Whole Body γ-Irradiation Induced Oxidative Stress and Clastogenic Damage in Mice by Ginger Essential Oil
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Protection against Whole Body γ-Irradiation Induced Oxidative Stress and Clastogenic Damage in Mice by Ginger Essential Oil
Jeena, Kottarapat; Liju, Vijayasteltar B; Ramanath, Viswanathan; Kuttan, Ramadasan;
  PDF(new window)
 Abstract
Radioprotective effects of ginger essential oil (GEO) on mortality, body weight alteration, hematological parameters, antioxidant status and chromosomal damage were studied in irradiated mice. Regression analysis of survival data in mice exposed to radiation yielded LD50/30 as 7.12 and 10.14 Gy for control (irradiation alone) and experimental (GEO-treated irradiated) mice, respectively, with a dose reduction factor (DRF) of 1.42. In mice exposed to whole-body gamma-irradiation (6 Gy), GEO pre-treatment at 100 and 500 mg/kg b.wt (orally) significantly ameliorated decreased hematological and immunological parameters. Radiation induced reduction in intestinal tissue antioxidant enzyme levels such as superoxide dismutase, catalase, glutathione peroxidase and glutathione was also reversed following administration of GEO. Tissue architecture of small intestine which was damaged following irradiation was improved upon administration of GEO. Anticlastogenic effects of GEO were studied by micronuclei assay, chromosomal aberration and alkaline gel electrophoresis assay. GEO significantly decreased the formation of micronuclei, increased the P/N ratio, inhibited the formation of chromosomal aberrations and protected agaisnt cellular DNA damage in bone marrow cells as revealed by comet assay. These results are supportive of use of GEO as a potential radioprotective compound.
 Keywords
Ginger essential oil;radioprotection;dose reduction factor;comet assay-chromosomal;
 Language
English
 Cited by
 References
1.
Aebi M (1974). Catalase estimation. In 'Methods of enzymatic analysis', Eds Berg Meyer HV. Verlag, Chemie, New York, 673-84.

2.
Bancroft JD, Cook HF (1984). Manual of Histologic Techniques. Churchill Livingston, London, 171-4.

3.
Drabkin DL, Austin M (1932). Spectrometric studies; spectrometric constants for common haemoglobin derivatives in human, dog and rabbit blood. J Biol Chem, 98, 719-33.

4.
Gandhi NM, Maurya DK, Salvi VP, et al (2004). Radioprotection of DNA by glycyrrhizic acid through scavenging free radicals. J Radiat Res, 45, 461-8. crossref(new window)

5.
Gurdip S, Kapoor IPS, Pratibha S, et al (2008). Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food Chem Toxicol, 46, 3295-02. crossref(new window)

6.
Hafeman DG, Sundae RA, Houestra WG (1974). Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutr, 104, 580-7. crossref(new window)

7.
Harikumar KB, Kuttan, R (2004). Protective effect of an extract of Phyllanthus amarus against radiation induced damage in mice. J Radiat Res, 45, 133-9. crossref(new window)

8.
Indu T, Uma Devi P, Papiya B (2011). Protection against radiation clastogenicity in mouse bone marrow by Phyllanthus niruri. Indian J Exp Biol, 49, 704-10.

9.
Jeena K, Liju VB, Kuttan R (2011). A preliminary 13-week oral toxicity study of ginger oil in male and female wistar rats. Int J Toxicol, 30, 662-70. crossref(new window)

10.
Jeena K, Liju VB, Viswanathan R, Kuttan R (2014).Antimutagenic potential and modulation of carcinogen-metabolizing enzymes by ginger essential oil. Phytother Res, 28, 849-55. crossref(new window)

11.
Jeena K, Liju VB, Kuttan R (2013). Antioxidant, anti-inflammatory and antinociceptive activities of essential oil from ginger. Indian J Physiol Pharmacol, 57, 51-62.

12.
Koch C, Reichling J, Schneele J (2008). Inhibitory effect of essential oils against herpes simplex virus type-2. Phytomedicine, 15, 71-80. crossref(new window)

13.
Konca K, Lankoff A, Banasik A, et al (2003). A cross platform public domain pc image analysis program for the comet assay. Mutat Res, 534, 15-20. crossref(new window)

14.
Londhe JS, Devasagayam TP, Foo LY, et al (2009). Radioprotective properties of polyphenols from Phyllanthus amarus Linn. J Radiat Res, 50, 303-9. crossref(new window)

15.
Lowry OH, Rosenbrough NJ, Farr AL, et al (1951). Protein measurement with Folin Wu reagent. J Biol Chem, 193, 265-75.

16.
Mackova P, Fedoroeko (2002). Effect of Liposomal muramyl tripeptide phosphatidylethanolamine and Indomethacin on hematopoietic recovery in irradiated mice. Physiol Res, 51, 511-21.

17.
Mc Cord JM, Fridovich I (1969). Superoxide dismutase enzyme function for erythrocaprein. J Biochem, 244, 6049-56.

18.
Moron MA, Depierre JW, Manner Vick B (1979). Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat liver. Biochim Biophys Acta, 582, 67-8. crossref(new window)

19.
Nair CKK, Salvi VP (2005). Protection of DNA from gamma radiation induced strand breaks by epicatechin. Mutat Res, 650, 48-54.

20.
Ping X, Junqing J, Junfeng J, et al (2011). Radioprotective effects of troxerutin against gamma irradiation in V79 cells and mice. Asian Pac J Cancer Prev, 12, 2593-6.

21.
Ronald EC, Annette MB, Susan MB, et al (2008). Resveratrol Reduces Radiation-Induced Chromosome Aberration Frequencies in Mouse Bone Marrow Cells. Radiat Res, 169, 633-8. crossref(new window)

22.
Schmid W (1975). The micronucleus test. Mutat Res, 31, 9-15. crossref(new window)

23.
Singh G, Maurya S, Catalan C, et al (2005). Studies on essential oils, Part 42: chemical, antifungal, antimicrobial and sprout suppressant studies on ginger essential oil and its oleoresin. Flavour Frag J, 20, 1-6. crossref(new window)

24.
Singh NP (2000). Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat Res, 455, 111-27. crossref(new window)

25.
Song LH, Yan HL, Cai DL (2006). Protective effects of soybean isoflavone against gamma-irradiation induced damages in mice. J Radiat Res, 47, 157-65. crossref(new window)

26.
Thulasi GP, Damodharan DP (2007). Prevention of radiation-induced chromosome damage in mouse bone marrow by aqueous leaf extract of Chicorium intybus. J Cell Molecular Biol, 6, 59-64.

27.
Vendruscolo A, Takaki I, Bersani-Amado LE, et al (2006). Antiinflammatory and antinociceptive activities of Zingiber officinale Roscoe essential oil in experimental animal models. Ind Jour Pharm, 8, 58-9.

28.
Wang K, Liu C, Di CJ, et al (2014). Kojic acid protects C57BL/6 mice from gamma-irradiation induced damage. Asian Pac J Cancer Prev, 15, 291-7. crossref(new window)

29.
Weiss JF, Landauer MR (2003). Protection against ionizing radiation by antioxidants, nutrients and phytochemicals. Toxicol, 189, 1-30. crossref(new window)

30.
Xu P, Zhang WB, Cai XH, et al (2014). Flavonoids of Rosa roxburghii Tratt act as radioprotectors. Asian Pac J Cancer Prev, 15, 8171-5. crossref(new window)