Advanced SearchSearch Tips
Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells
Qian, Jun; Li, Jing; Jia, Jian-Guang; Jin, Xin; Yu, Da-Jun; Guo, Chen-Xu; Xie, Bo; Qian, Li-Yu;
  PDF(new window)
Objectives: To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells. Materials and Methods: SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting. Results: It was found that the proliferation of SP was significantly faster than that of NSP (P<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (P<0.05). Conclusions: With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.
Gastric cancer;side population cells;ginsenoside -rh2;proliferation;apoptosis;
 Cited by
Akagi H, Higuchi H, Sumimoto H, et al (2013). Suppression of myeloid cell leukemia-1 (Mcl-1) enhances chemotherapyassociated apoptosis in gastric cancer cells. Gastric Cancer, 16, 100-10. crossref(new window)

Choi S, Oh JY and Kim SJ (2011). Ginsenoside Rh2 induces Bcl-2 family proteins-mediated apoptosis in vitro and in xenografts in vivo models. J Cell Biochem, 112, 330-40. crossref(new window)

Goodell MA, Brose K, Paradis G, et al (1996). Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med, 183, 1797-806. crossref(new window)

Kato K, Gong J, Iwama H, et al (2012). The antidiabetic drug metformin inhibits gastric cancer cell proliferation in vitro and in vivo. Mol Cancer Ther, 11, 549-60. crossref(new window)

Kong X, Ma MZ, Zhang Y, et al (2014). Differentiation therapy: sesamin as an effective agent in targeting cancer stem-like side population cells of human gallbladder carcinoma. BMC Complement Altern Med, 14, 254. crossref(new window)

Li B, Zhao J, Wang CZ, et al (2011). Ginsenoside Rh2 induces apoptosis and paraptosis-like cell death in colorectal cancer cells through activation of p53. Cancer Letters, 301, 185-92. crossref(new window)

Nakata H, Kikuchi Y, Tode T, et al (1998). Inhibitory effects of ginsenoside Rh2 on tumor growth in nude mice bearing human ovarian cancer cells. Jpn J Cancer Re, 89, 733-40. crossref(new window)

Qian J, Xie H, Guo, CX, et al (2015). Sijunzi decoction demolition parties inhibit proliferation and induce apoptosis of human gastric cancer BGC-823 side population. Afr J Tradit Complement Altern Med, 12, 77-89. crossref(new window)

Saricanbaz I, Karahacioglu E, Ekinci O, et al (2014). Prognostic Significance of Expression of CD133 and Ki-67 in Gastric Cancer. Asian Pac J Cancer Prev, 15, 8215-9. crossref(new window)

Shaharuddin B, Harvey I, Ahmad S, et al (2014). Characterisation of human limbal side population cells isolated using an optimised protocol from an immortalised epithelial cell line and primary limbal cultures. Stem Cell Rev, 10, 240-50. crossref(new window)

Tang XP, Tang GD, Fang CY, et al (2013). Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells. World J Gastroenterol, 19, 1582-92. crossref(new window)

Wang YX, Cai H, Jiang G, et al (2014). Silibinin inhibits proliferation, induces apoptosis and causes cell cycle arrest in human gastric cancer MGC803 cells via STAT3 pathway inhibition. Asian Pac J Cancer Prev, 15, 6791-8. crossref(new window)

Zheng T, Meng X, Wang JB, et al (2010). PTEN-and p53-mediated apoptosis and cell cycle arrest by FTY720 in gastric cancer cells and nude mice. J Cell Biochem, 111, 218-28. crossref(new window)