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Neural Transdifferentiation: MAPTau Gene Expression in Breast Cancer Cells
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 Title & Authors
Neural Transdifferentiation: MAPTau Gene Expression in Breast Cancer Cells
Lara-Padilla, E; Miliar-Garcia, A; Gomez-Lopez, M; Romero-Morelos, P; Bazan-Mendez, CI; Alfaro-Rodriguez, A; Anaya-Ruiz, M; Callender, K; Carlos, A; Bandala, C;
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Background: In tumor cells, aberrant differentiation programs have been described. Several neuronal proteins have been found associated with morphological neuronal-glial changes in breast cancer (BCa). These neuronal proteins have been related to mechanisms that are involved in carcinogenesis; however, this regulation is not well understood. Microtubule-associated protein-tau (MAP-Tau) has been describing in BCa but not its variants. This finding could partly explain the neuronal-glial morphology of BCa cells. Our aim was to determine mRNA expression of MAP-tau variants 2, 4 and 6 in breast cancer cell lines. Materials and Methods: Cultured cell lines MCF-10A, MDA-MB-231, SKBR3 and T47D were observed under phase-contrast microscopy for neural morphology and analyzed for gene expression of MAP-Tau transcript variants 2, 4 and 6 by real-time PCR. Results: Regarding morphology like neural/glial cells, T47D line shown more cells with these features than MDA-MB-231 and SKBR. In another hand, we found much greater mRNA expression of MAP-Tau transcript variants 2, and to a lesser extent 4 and 6, in T47D cells than the other lines. In conclusion, regulation of MAP-Tau could bring about changes in cytoskeleton, cell morphology and motility; these findings cast further light on neuronal transdifferentiation in BCa.
Breast cancer;neural transdifferentiation;neuron/glial-like cell;microtubule-associated protein tau;
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