An Experimental Study on the Freeze Drying Process for Poly γ Glutamic Acid

Title & Authors
An Experimental Study on the Freeze Drying Process for Poly γ Glutamic Acid
Kang, Jisu; Sim, Yeon-Ho; Byun, Si-Ye; Chang, Young Soo; Kang, Byung Ha;

Abstract
This paper presents an experimental study on the freeze drying process for poly $\small{{\gamma}}$ glutamic acid. The physical properties of poly $\small{{\gamma}}$ glutamic acid are measured during the freeze-drying process. The moisture contents of poly $\small{{\gamma}}$ glutamic acid according to the glass transient temperature are obtained by DSC (Differential Scanning Calorimetry) analysis. The end point of primary drying for the poly $\small{{\gamma}}$ glutamic acid with a thickness of 3 mm is obtained by measuring the thickness of the dried layer, the amount of moisture evaporation, the moisture content, and the pressure in the drying vacuum chamber during the freeze-drying process. By considering the variation in the glass transient temperature with respect to the moisture content of the material, a control schedule for the heating plate temperature is suggested during the secondary drying process.
Keywords
Vacuum freeze drying;Biomaterial;Poly $\small{{\gamma}}$ glutamic acid;Glass transient temperature;
Language
Korean
Cited by
References
1.
Franks, F., 1998, Freeze-drying of bioproducts:putting principles into practice, European Journal of Pharmaceutics and Biopharmaceutics, Vol. 45, No. 3, pp. 221-229.

2.
Adams, G. D. J., Cook, I., and Ward, K. R., 2014, The Principles of Freeze-Drying, Methods in Molecular Biology, Vol. 1257, pp. 121-143.

3.
Kang, J. Y., 2004, Automatic control of pharmaceutical vacuum freeze dryer, Dongguk Graduate school.

4.
Park, S. J. and Song, C. S., 2003, Vacuum freezing dryer, Korean Journal of Air-Conditioning and Refrigeration Engineering, pp. 29-40.

5.
Sung, M. H. and Park, C., 2009, New bioindustrial development of high molecular weight of poly-gamma-glutamic acid produced by Bacillus subtilis(chungkookjang), Polymer Science and Technology, Vol. 20, No. 5, pp. 440-446.

6.
Jung, G. H., Moon, S. H., Choi, J. H., Lee, J. W., and Kim, N. I., 2011, Poly-${\gamma}$-glutamic acid modifies cytokine release in vitro, J Kyung Hee Univ Med cent, Vol. 27, No. 1, pp. 34-42.

7.
Ogunleye, A., Bhat, A., Irorere, V. U., Hill, D., Williams, C., and Radecka, I., Poly-${\gamma}$-glutamic acid production, properties and applications, Faculty of Science and Engineering, University of Wolverhampton, Wulfuruna street, Wolverhampton, UK.

8.
Park, H. Y., 1990, Freezing dryer, The magazine of the Korea refrigerating and air conditioning technology association, Vol. 7, No. 9, pp. 42-57.

9.
Park, N. H., Kim, B. S., Kang, T. S., and Shin, D. H., 1998, Modification of conventional freeze dryer, Korean J. Food SCI. Technol, Vol. 20, No. 3, pp. 350-356.

10.
Roy, M. L. and Pikal, M. J., 1989, Process control in freeze drying:determination of the end point of sublimation drying by an electronic moisture sensor, Pharmaceutical Science and Technology, Vol. 43, No. 2, pp. 60-66.

11.
Bhandrari, B. R. and Howes, T., 1999, Implication of glass transition for the drying and stability of dried foods, Journal of Food Engineering, Vol 40, pp. 71-79.

12.
Lee, S. J., 2004, Considertion for the optimum operation of vacuum freeze drying, Food Engineering Progress, Vol. 8, No. 3, pp. 147-157.

13.
Tang, X. and Pikal, M. J., 2004, Design of freeze drying process for pharmaceuticals:practical advice, Pharmaceutical research, Vol. 21, No. 2, pp. 191-200.

14.
Pikal, M. J., Shah, S., Roy, M. L., and Putman, R., 1990, International Journal of Pharmaceutics, The secondary drying stage of freeze drying:drying kinetics as a function of temperature and chamber preesure, Vol. 60, No. 3, pp. 203-207.

15.
Zhai, S., Taylor, R., Sanches, R., and Slater, N. K. H., 2003, Measurement of lyophilisation primary drying by freeze-drying microscopy, Chemical Engineering Science, Vol. 58, pp. 2313-2323.