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Effects of Carbenoxolone and P2X recepter antagonist combined therapy on oral neuropathic pain in rat
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 Title & Authors
Effects of Carbenoxolone and P2X recepter antagonist combined therapy on oral neuropathic pain in rat
Ku, In-Young; Moon, Seon-Jeong; Ka, Kyung-Hwan; Park, Min-Kyoung;
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 Abstract
The study was conducted to confirm the analgesic effects of the Carbenoxolone(CBX)and P2X receptor antagonist(iso-PPADS), which separates the gap junction in the facial neuropathic pain model. The experiment used white male Sprague-Dawley rats (240~280g). The second left molars on the lower jaw was extracted to induce facial neuropathic pain, and small dental implants were implanted to induce damage to the inferior alveolar nerve. When CBX was injected twice daily to the abdominal cavity, a significant analgesic effect at 5ug/kg was observed(p<0.05). In addition, when iso-PPADS was injected twice daily into the abdominal cavity, a significant analgesic reaction was observed at (p<0.05). When the two drugs were injected together at a low concentration, in which they did not display an effect, they displayed a significant analgesic reaction at CBX 1ug/kg and iso-PPADS 2.5ug/kg(p<0.05). When a gap injunction block using a low concentration of CBX and a low concentration P2X receptor antagonist was injected together, the pain suppressing effect was observed against the orofacial neuropathic pain mechanism. These results make it possible to determine that the gap junction block using CBX and the injection of the P2X receptor antagonist plays an important role in the pain management of the facial region.
 Keywords
Gap junction;Carbenoxolone;P2x recepter antagonist;
 Language
Korean
 Cited by
 References
1.
M. T. Mendlik, T. J. Uritsky, Treatment of Neuropathic Pain. Curr Treat Options. Neurol, Vol.50, No.12, 2015. DOI: http://dx.doi.org/10.1007/s11940-015-0381-2

2.
C. J. Woolf, Physiological, inflammatory and neuropathic pain. Adv Tech Stand Neurosurg. Vol.15, pp39-62. 1987. DOI: http://dx.doi.org/10.1007/978-3-7091-6984-1_2 crossref(new window)

3.
Y. Ge, F. Wu, X. Sun, Z. Xiang, L. Yang, S. Huang, Z. Lu, Y. Sun, W. F. Yu, Intrathecal infusion of hydrogen-rich normal saline attenuates neuropathic pain via inhibition of activation of spinal astrocytes and microglia in rats. PLoS One. Vol.23, No.5. ppe97436.2014. DOI: http://dx.doi.org/10.1371/journal.pone.0097436 crossref(new window)

4.
K. L. Sato, L. M. Johanek, L. S. Sanada, K. A. Sluka, Spinal cord stimulation reduces mechanical hyperalgesia and glial cell activation in animals with neuropathic pain. Anesth Analg .Vol.118, No.2. pp 464-472. 2014. DOI: http://dx.doi.org/10.1213/ANE.0000000000000047 crossref(new window)

5.
S. Lee, Y. Q. Zhao, A. Ribeiro-da-Silva, J. Zhang, Distinctive response of CNS glial cells in oro-facial pain associated with injury, infection and inflammation. Mol Pain. Vol.79, No.6. 2010. DOI: http://dx.doi.org/10.1186/1744-8069-6-79

6.
R. S. Tonkin, Y. Mao, S. J. O'Carroll, L. F. Nicholson, C. R. Green, C. A. Gorrie, Moalem-Taylor G. Gap junction proteins and their role in spinal cord injury. Front Mol Neurosci. Vol.7, No.6. 2015. DOI: http://dx.doi.org/10.3389/fnmol.2014.00102 crossref(new window)

7.
Q. Chang, R. J. Balice-Gordon, Gap junctional communication among developing and injured motor neurons. Brain Res. Vol.32, pp242-249. 2000. DOI: http://dx.doi.org/10.1016/S0165-0173(99)00085-5 crossref(new window)

8.
Q. Chang, M. Gonzalez, M. J. Pinter, R. J. Balice-Gordon, Gap junctional coupling and patterns of connexin expression among neonatal rat lumbar spinal motor neurons. J. Neurosci. Vol.19, pp10813-10828. 1999.

9.
M. V. Bennett, J. M. Garre, J. A. Orellana, F. F. Bukauskas, M. Nedergaard, C. Giaume, Connexin and pannexin hemichannels in inflammatory responses of glia and neurons. Brain Res. Vol.1487. pp3-15. 2012 DOI: http://dx.doi.org/10.1016/j.brainres.2012.08.042 crossref(new window)

10.
M. J. Chen, B. Kress, X. Han, K. Moll, W. Peng, R. R. Ji, Astrocytic CX43 hemichannels and gap junctions play a crucial role in development of chronic neuropathic pain following spinal cord injury. Glia. Vol.60, pp1660-1670. 2012. DOI: http://dx.doi.org/10.1002/glia.22384 crossref(new window)

11.
Y. H. Jeon, D. H. Youn, Spinal Gap Junction Channels in Neuropathic Pain. Korean J Pain. Vol.28, No.4, pp.231-235. 2015. DOI: http://dx.doi.org/110.3344/kjp.2015.28.4.231 crossref(new window)

12.
O. Chever, C.-Y. Lee, N. Rouach, Astroglial connexin43 hemichannels tune basal excitatory synaptic transmission. J. Neurosci. Vol.34 pp.11228-11232.2014 DOI: http://dx.doi.org/10.1523/JNEUROSCI.0015-14 crossref(new window)

13.
Burnstock G. Physiology and pathophysiology of purinergic neurotransmission. Physiol Rev. Vol.12 pp.659-797. 2007. DOI: http://dx.doi.org/10.1152/physrev.00043.2006

14.
M. K. Lee, S. R. Han, M. K. Park, M. J. Kim, Y. C. Bae, S. K. Kim, J. S. Park, D. K. Ahn, Behavioral evidence for the differential regulation of p-p38 MAPK and p-NF-${\kappa}B$ in rats with trigeminal neuropathic pain. Mol Pain. Vol.5. No. 7.pp.57-61.2011. DOI: http://dx.doi.org/10.1186/1744-8069-7-57

15.
H. R. Han, S .P. Yeo, M. K. Lee, Y. C. Bae, D. K. Ahn, Early dexamethasone relieves trigminal neuropathic pain. J Dent Res. Vol. 89, No. 9. pp 915-920. 2010. DOI: http://dx.doi.org/10.1177/0022034510374056 crossref(new window)

16.
M. Kamalpour, M. Fereidoni, A. Moghimi. Effects of intrathecal carbenoxolone treatment on nociception and analgesia in rat. Balkan Med J. Vol. 31, No. 2. pp164-172. 2014. DOI: http://dx.doi.org/10.5152/balkanmedj.2014.13099 crossref(new window)

17.
Q. Xu, Y. K. Cheong, F. Yang, V. Tiwari, J. Li, J. Liu, S. N. Raja, W. Li, Y. Guan, Intrathecal carbenoxolone inhibits neuropathic pain and spinal wide-dynamic range neuronal activity in rats after an L5 spinal nerve injury. Neurosci Lett. Vol. 563, No. 20. pp 45-50.2014. DOI: http://dx.doi.org/10.1016/j.neulet.2014.01.042. crossref(new window)

18.
M. P. Abbracchio, G. Burnstock, Purinergic signalling: pathophysiological roles. Jpn J Pharmacol. Vol.78, pp113-45. 1998. DOI: http://dx.doi.org/10.1254/jjp.78.113 crossref(new window)

19.
Chessell IP, Hatcher JP, Bountra C, Michel AD, Hughes JP, Green P, Egerton J, Murfin M, Richardson J, Peck WL, Grahames CB, Casula MA, Yiangou Y, Birch R, Anand P, Buell GN. Disruption of the P2X7 purinoceptor gene abolishes chronic inflammatory and neuropathic pain. Pain. Vol.114, pp386-96. 2005. DOI: http://dx.doi.org/10.1016/j.pain.2005.01.002 crossref(new window)

20.
W. Li, J. Li, A. E. Sama, H. Wang. Carbenoxolone blocks endotoxin-induced protein kinase R (PKR) activation and high mobility group box 1 (HMGB1) release. Mol Med. Vol.19, pp203-211, 2013. DOI: http://dx.doi.org/10.2119/molmed.2013.00064 crossref(new window)

21.
G. CHRISTIAN, V. LAURENT, Intercellular Calcium Signaling and Gap Junctional Communication in Astrocytes. Glia. Vol.24, pp50-64, 1998. DOI: http://dx.doi.org/10.1002/(SICI)1098-1136(199809)24:1%3C50::AID-GLIA6%3E3.3.CO;2-Z crossref(new window)