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Effect of salinity and used medium on the induction of sexual reproduction in the rotifer Brachionus rotundiformis
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 Title & Authors
Effect of salinity and used medium on the induction of sexual reproduction in the rotifer Brachionus rotundiformis
Lee, Kyun-Woo; Sim, So-Myung;
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This study examined the effects of salinity and medium used on the induction of sexual reproduction for the mass production of the resting eggs in the marine rotifer Brachionus rotundiformis for 9 days. After exposure to various salinities, the mixis rate in all treatments began to increase on the third day and decreased after the 4th or 5th day. The resting eggs appeared on the 4th day at 10‰, 15‰, 20‰, 25‰. In particular, the fertilization rates with 33.3% and 31.3% at 20‰ and 25‰ on day 6 were higher than those of the others, respectively. Mean mixis rate for 9 day at 0‰ and 20‰ were the highest with 25.3% and 20.2%, respectively, and the fertilization rate (14.0%) at 25‰ was the highest. In the medium experiment used, the mixis rate in all treatments were similar to the results of the salinity experiment. The resting eggs were only found at 0% and 25% of the used medium treatments. While there were no significant differences in the mean fertilization rate of all treatments, the mean mixis rate (22.3%) in the 25% treatment was higher than that of the control (0% treatment). As a result, salt stress was more effective than using the used medium to induce sexual reproduction in B. rotundiformis. The optimal salinity was considered to be 20-25‰, which caused a high mixis and fertilization rate in the rotifer. These results provide basic data for the mass production of rotifer resting eggs.
Brachionus rotundiformis;Salinity;Used medium;Sexual production;Resting egg;
 Cited by
기수산 요각류 Paracyclopina nana의 대량배양,이균우;최영웅;

한국산학기술학회논문지, 2016. vol.17. 8, pp.262-266 crossref(new window)
J. J. Gilbert, "Environmental and endogenous control of sexuality in a rotifer life cycle: developmental and population biology", Evol. Dev. 5, 19-24. 2003. DOI: crossref(new window)

J. J. Gilbert and M. C. Dieguez, "Low crowding threshold for induction of sexual reproduction and diapause in a Patagonian rotifer", Freshw. Biol. 55, 1705-1718. 2010. DOI: crossref(new window)

S. J. Hanson, C. P. Stelzer, D. B. M. Welch and J. M. Logsdon, "Comparative transcriptome analysis of obligately asexual and cyclically sexual rotifers reveals genes with putative functions in sexual reproduction, dormancy, and asexual egg production", Bmc Genomics 14 2013. DOI: crossref(new window)

H. J. Kim, K. Suga, B. M. Kim, J. S. Rhee, J. S. Lee and A. Hagiwara, "Light-dependent transcriptional events during resting egg hatching of the rotifer Brachionus manjavacas", Mar Genom 20, 25-31. 2015. DOI: crossref(new window)

C. Gabaldon and M. J. Carmona, "Allocation patterns in modes of reproduction in two facultatively sexual cryptic rotifer species", J. Plankton. Res. 37, 429-440. 2015. DOI: crossref(new window)

V. Cruciani, C. Iovine, J. P. Thome and C. Joaquim-Justo, "Impact of three phthalate esters on the sexual reproduction of the Monogonont rotifer, Brachionus calyciflorus" Ecotoxicology 25, 192-200. 2016. DOI: crossref(new window)

S. B. Hur and H. G. Park, "Size and resting egg formation of Korean Rotifer, Brachionus plicatilis and B. calyciflorus", J. Aquacult. 9, 187-194. 1996.

H. G. Park and S. B. Hur, "Production and hatching rate of resting egg of Korean rotifer, Brachionus plicatilis (S-type) with different diets", J. Aquacult. 9, 329-337. 1996.

T. W. Snell and N. J. D. DesRosiers, "Effect of progesterone on sexual reproduction of Brachionus manjavacas (Rotifera)", J. Exp. Mar. Biol. Ecol. 363, 104-109. 2008. DOI: crossref(new window)

T. W. Snell, J. Kubanek, W. Carter, A. B. Payne, J. Kim, M. K. Hicks and C. P. Stelzer, "A protein signal triggers sexual reproduction in Brachionus plicatilis (Rotifera)", Mar. Biol. 149, 763-773. 2006. crossref(new window)

K. W. Lee, J. H. Kang and H. G. Park, "Effect of food concentration on grazing, growth and fecundity of cyclopoid copepod Paracyclopina nana", J. Korea Acad. Industr. Coop. Soc. 13, 5206-5210. 2012. DOI: crossref(new window)

H. G. Park, "Growth and production of resting eggs of freshwater rotifer, Brachionus calyciflorus Pallas at the different temperatures", J. Korean Fish. Soc. 31, pp. 779-784, 1998.

H. G. Park and S. B. Hur, "Resting egg production of six strains of Korean rotifer, Brachionus plicatilis (S-type)", J. Aquacult. 9, pp. 195-203, 1996.

T. W. Snell and E. M. Boyer, "Thresholds for mictic female production in the rotifer Brachionus plicatilis (Muller)", J. Exp. Mar. Biol. Ecol. 124, 73-85. 1988. DOI: crossref(new window)

T. Schroder, "Precopulatory mate guarding and mating behaviour in the rotifer Epiphanes senta (Monogononta : Rotifera)", Proc. R. Soc. Lond. B 270, 1965-1970. 2003. DOI: crossref(new window)

K. W. Lee, H. G. Park, S.-M. Lee, H.-S. Han and Y. S. Lim, "Food value or freshwater rotifer (Brachionus calyciflorus) for culture of sweetfish (Plecoglossus altivelis) Larvae", J. Kor. Fish. Soc. 37, 7-12. 2004. DOI: crossref(new window)

H. J. Kim and A. Hagiwara, "Effect of salinity during resting egg formation and hatching on descendent reproduction in the rotifer Brachionus rotundiformis Tschugunoff", J. Plankton. Res. 33, 1033-1042. 2011. DOI: crossref(new window)

T. W. Snell, "Chemical ecology of rotifers", Hydrobiologia 387/388, 267-276. 1998. DOI: crossref(new window)

N. Y. Denekamp, M. A. S. Thorne, M. S. Clark, M. Kube, R. Reinhardt and E. Lubzens, "Discovering genes associated with dormancy in the monogonont rotifer Brachionus plicatilis", BMC Genomics 10, -. 2009.

T. W. Snell, R. Ricomartinez, L. N. Kelly and T. E. Battle, "Identification of a sex-pheromone from a rotifer", Mar. Biol. 123, pp. 347-353, 1995. DOI: crossref(new window)

T. Snell, T. Shearer, H. Smith, J. Kubanek, K. Gribble and D. Welch, "Genetic determinants of mate recognition in Brachionus manjavacas (Rotifera)", BMC Biol. 7, 2009. DOI: crossref(new window)

E. P. Stout, J. J. L. Clair, T. W. Snell, T. L. Shearer, and J. Kubanek, "Conservation of progesterone hormone function in invertebrate reproduction", Proc. Natl. Acad. Sci. USA 107, 11859-11864 2010. DOI: crossref(new window)