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A Simple and Reliable Molecular Detection Method for Tomato yellow leaf curl virus in Solanum lycopersicum without DNA Extraction
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  • Journal title : Research in Plant Disease
  • Volume 21, Issue 3,  2015, pp.180-185
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.3.180
 Title & Authors
A Simple and Reliable Molecular Detection Method for Tomato yellow leaf curl virus in Solanum lycopersicum without DNA Extraction
Yoon, Ju-Yeon; Kim, Su; Choi, Gug-Seoun; Choi, Seung-Kook;
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 Abstract
In the present work, a pair of primers specific to Tomato yellow leaf curl virus (TYLCV) was designed to allow specific amplification of DNA fragments from any TYLCV isolates using an extensive alignment of the complete genome sequences of TYLCV isolates deposited in the GenBank database. A pair of primers which allows the specific amplification of tomato -tubulin gene was also analyzed as an internal PCR control. A duplex PCR method with the developed primer sets showed that TYLCV could be directly detected from the leaf crude sap of infected tomato plants. In addition, our developed duplex PCR method could determine PCR errors for TYLCV diagnosis, suggesting that this duplex PCR method with the primer sets is a good tool for specific and sensitive TYLCV diagnosis. The developed duplex PCR method was further verified from tomato samples collected from some farms in Korea, suggesting that this developed PCR method is a simple and reliable tool for rapid and large-scale TYLCV detections in tomato plants.
 Keywords
Detection;Duplex PCR;Internal Control;Tomato;TYLCV;
 Language
English
 Cited by
 References
1.
Aidawati, N., Hidayat, S. H., Suseno, R. and Sosromarsono, S. 2002. Transmission of an Indonesian isolate of Tobacco leaf curl virus (Geminivirus) by Bemisia tabaci Genn. (Hemiptera: Aleyrodidae). Plant Pathol. J. 18: 231-236. crossref(new window)

2.
Al-Bitar, L. and Luisoni, E. 1995. Tomato yellow leaf curl geminivirus: serological evaluation of an improved purification method. EPPO Bull. 25: 269-276. crossref(new window)

3.
Atzmon, G., van Oss, H. and Czosnek, H. 1998. PCR amplification of tomato yellow leaf curl virus (TYLCV) DNA from squashes of plants and whitefly vectors: Application to the study of TYLCV acquisition and transmission. Eur. J. Plant Pathol. 104: 189-194. crossref(new window)

4.
Brown, J. K. and Idris, A. M. 2008. Introduction of the exotic monopartite Tomato yellow leaf curl virus into west coast Mexico. Plant Dis. 90: 1360.

5.
Choi, S. K., Choi, H. S., Yang, E. Y., Cho, I. S., Cho, J. D. and Chung, B. N. 2013. Construction of tomato yellow leaf curl virus clones for resistance assessment in tomato plants. Korean J. Hort. Sci. Technol. 31: 246-254. crossref(new window)

6.
Cohen, S. and Harpaz, I. 1964. Periodic, rather than continual acquisition of new tomato virus by its vector, the tobacco whitefly (Bemisia tabaci Gennadius). Ent. Exp. Applicata 7: 155-166. crossref(new window)

7.
Czosnek, H. 2007. Tomato yellow leafcurl disease. Springer, Netherlands.

8.
Czosneck, H. and Laterrot, H. 1997. A worldwide survey of tomato yellow leaf curl viruses. Arch. Virol. 142: 1391-1406. crossref(new window)

9.
Di Martino, M. T., Albanese, G., Silvestro, I. and Catara, A. 1993. Rapid detection of tomato yellow leaf curl virus in plants by polymerase chain reaction. Riv. Pat. Veg. 3: 35-40.

10.
Duffy, S. and Holmes, E. 2007. Multiple introduction of the old world Begomovirus Tomato yellow leaf curl virus into the new world. Amer. Soc. Microbiol. 73: 7114-7117.

11.
Ghanim, M. and Czosnek, H. 2000. Tomato yellow leaf curl geminivirus (TYLCV-Is) is transmitted among whiteflies (Bemisia tabaci) in a sex-related manner. J. Virol. 74: 4738-4745. crossref(new window)

12.
Ghanim, M., Morin, S., Zeidan, M. and Czosneck, H. 1988. Evidence for transovarial transmission of tomato yellow leaf curl virus by its vector, the whitefly Bemisia tabaci. Virology 240: 295-303.

13.
Innis, M. A. and Gelfand, D. H. 1990. Optimization of PCRs. In: PCR Protocols. A Guide to Methods and Applications, eds. by M. A. Gelfand, D. H. Sninsky and T. J. White, pp. 3-20. Academic Press, San Diego, USA.

14.
Lee, H., Song, W., Kwak, H. R., Kim, J. D., Park, J. G., Auh, C. K., Kim, D. H., Lee, S. C., Lee, S. C. and Choi, H. S. 2010. Phylogenetic analysis and inflow route of Tomato yellow leaf curl virus (TYLCV) and Bemisia tabaci in Korea. Mol. Cells 30: 467-476. crossref(new window)

15.
Martinez-Zubiaur, Y., Zabalgogeazcoa, I., De Blas, C., Sanchez, F., Peralta, E. L., Romero, J. and Ponz, F. 1996. Geminiviruses associated with diseased tomatoes in Cuba. J. Phytopathology 144: 277-279. crossref(new window)

16.
Maruthi, M. N., Alam, S. N., Kader, K. A., Rekha, A. R., Cork, A. and Colvin, J. 2005. Nucleotide sequencing, whitefly transmission and screening tomato for resistance against two newly described Begomoviruses in Bangladesh. Phytopathology 95: 1472-1481. crossref(new window)

17.
Momol, M. T., Simone, G. W., Dankers, W., Sprenkel, R. K., Olson, M., Momol, E. A., Polston, J. E. and Hiebert, E. 1999. First report of tomato yellow leaf curl virus in tomato in Georgia. Plant Dis. 83: 487.

18.
Moriones, E. and Navas-Castillo, J. 2000. Tomato yellow leaf curl virus, an a dead-end host for tomato yellow leaf curl virus. Phytopathology 95: 1089-1097.

19.
Nakhla, M. K., Mazyad, H. M. and Maxwell, D. P. 1993. Molecular characterisation of four tomato yellow leaf curl virus isolates from Egypt and development of diagnostic methods. Phytopathol. Mediterr. 32: 163-173.

20.
Navas-Castillo, J., Diaz, J. A., Sanchez-Campos, S. and Moriones, E. 1998. Improvement of the print-capture polymerase chain reaction procedure for efficient amplification of DNA virus genomes from plants and insect vectors. J. Virol. Methods 75: 195-198. crossref(new window)

21.
Navot, N., Ber, R. and Czosnek, H. 1989. Rapid detection of tomato yellow leaf curl virus in squashes of plant and insect vectors. Phytopathology 79: 562-568. crossref(new window)

22.
Navot, N., Pichersky, E., Zeidan, M., Zamir, D. and Czosneck, H. 1991. Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic component. Virology 185: 151-161. crossref(new window)

23.
Polston, J. E., McGovern, R. J. and Brown, L. G. 1999. Introduction of tomato yellow leaf curl virus in Florida and implications for the spread of this and other geminiviruses of tomato. Plant Dis. 83: 984-988. crossref(new window)

24.
Rojas, M. R., Gilbertson, R. L., Russell, D. R. and Maxwell, D. P. 1992. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis. 77: 340-347.

25.
Roye, M. E., Wernecke, M. E., McLaughlin, W. A., Nakhla, M. K. and Maxwell, D. P. 1999. Tomato dwarf leaf curl virus, a new bipartite geminivirus associated with tomatoes in Jamaica and mixed infection with tomato yellow leaf curl virus. Plant Pathol. 48: 370-378. crossref(new window)

26.
Tsai, W. S., Shih, S. L., Kenyon, L., Green, S. K. and Jan, F. J. 2011a. Temporal distribution and pathogenicity of the predominant tomato-infecting begomoviruses in Taiwan. Plant Pathol. 60: 787-799. crossref(new window)

27.
Tsai, W. S., Shih, S. L., Venkatesan, S. G., Aquino, M. U., Green, S. K., Kenyon, L. and Jan, F. J. 2011b. Distribution and genetic diversity of begomoviruses infecting tomto and pepper plants in the Philippines. Ann. Appl. Biol. 158: 275-287. crossref(new window)

28.
Valverde, R. A., Lotrakul, P. and Landry, A. D. 2001. First report of tomato yellow leaf curl virus in Louisiana. Plant Dis. 85: 230.

29.
Wu, J. X., Shang, H. L., Xie, Y. and Zhou, X. P. 2012. Monoclonal antibodies against the whitefly-transmitted tomato yellow leaf curl virus and their application in virus detection. J. Integr. Agri. 11: 263-268. crossref(new window)

30.
Wyatt, S. D. and Brown, J. K. 1996. Detection of subgroup III geminivirus isolated in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86: 1288-1293. crossref(new window)

31.
Xie, Y., Jiao, X., Zhou, X. Liu, H., Ni, Y. and Wu, J. 2013. Highly sensitive serological methods for detecting tomato yellow leaf curl virus in tomato plants and whiteflies. Virology J. 10: 142. crossref(new window)

32.
Yoon, J. Y., Choi, S. K., Palukaitis, P. and Gray, S. M. 2011. Agrobacteriummediated infection of whole plants by yellow dwarf viruses. Virus Res. 160: 428-434. crossref(new window)

33.
Zilberstein, A., Navot, N., Ovadia, S., Reinhartz, A., Herzberg, M. and Czosnek, H. 1989. Field-usable assay for diagnosis of the tomato yellow leaf curl virus in squashes of plants and insects by hybridization with a chromogenic DNA probe. Technique 1: 118-124.