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Development of an Efficient Simple Mass-Screening Method for Resistant Melon to Fusarium oxysporum f. sp. melonis
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  • Journal title : Research in Plant Disease
  • Volume 21, Issue 3,  2015, pp.201-207
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.3.201
 Title & Authors
Development of an Efficient Simple Mass-Screening Method for Resistant Melon to Fusarium oxysporum f. sp. melonis
Lee, Won Jeong; Jang, Kyoung Soo; Choi, Yong Ho; Kim, Heung Tae; Kim, Jin-Cheol; Choi, Gyung Ja;
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 Abstract
This study was conducted to establish a simple mass-screening method for resistant melon to Fusarium wilt caused by Fusarium oxysporum f. sp. melonis (FOM). Root-dipping inoculation method has been used to investigate resistance of melon plants to Fusarium wilt. However, the inoculation method requires a lot of labor and time because of complicate procedure. To develop a simple screening method on melon Fusarium wilt, occurrence of Fusarium wilt on susceptible and resistant cultivars of melon according to inoculation method including root-dipping, soil-drenching, tip, and scalpel methods was investigated. Scalpel and tip methods showed more clear resistant and susceptible responses in the melon cultivars than root-dipping inoculation method, but tip method represented slightly variable disease severity. In contrast, in the case of soil-drenching inoculation method, disease severity of the susceptible cultivars was very low. Thus we selected scalpel method as inoculation method of a simple screening method for melon Fusarium wilt. By using the scalpel inoculation method, resistance degrees of the cultivars according to incubation temperature after inoculation (25 and ) and inoculum concentration ( and ) were measured. The resistance or susceptibility of the cultivars was hardly affected by all the tested conditions. To look into the effectiveness of scalpel inoculation methods, resistance of 22 commercial melon cultivars to FOM was compare with root-dipping inoculation method. When the melon cultivars were inoculated by scalpel method, resistance responses of all the tested cultivars were clearly distinguished as by root-dipping method. Taken together, we suggest that an efficient simple mass-screening method for resistant melon plant to Fusarium wilt is to sow the seeds of melon in a pot (70 ml of soil) and to grow the seedlings in a greenhouse () for 7 days, to cut the root of seedlings with a scalpel and then pour a 10 ml-aliquot of the spore suspension of on soil. The infected plants were cultivated in a growth room at 25 to for about 3 weeks with 12-hr light a day.
 Keywords
Breeding;Disease resistance;Fusarium wilt;Inoculation method;Resistant screening;
 Language
Korean
 Cited by
1.
Establishing a high throughput screening method for large scale phenotyping of castor genotypes for resistance to Fusarium wilt disease, Phytoparasitica, 2016  crossref(new windwow)
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