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Influence of Commercial Antibiotics on Biocontrol of Soft Rot and Plant Growth Promotion in Chinese Cabbages by Bacillus vallismortis EXTN-1 and BS07M
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  • Journal title : Research in Plant Disease
  • Volume 21, Issue 4,  2015, pp.255-260
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.4.255
 Title & Authors
Influence of Commercial Antibiotics on Biocontrol of Soft Rot and Plant Growth Promotion in Chinese Cabbages by Bacillus vallismortis EXTN-1 and BS07M
Sang, Mee Kyung; Dutta, Swarnalee; Park, Kyungseok;
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 Abstract
We investigated influence of three commercial antibiotics viz., oxolinic acid, streptomycin, and validamycin A, on biocontrol and plant growth promoting activities of Bacillus vallismortis EXTN-1 and BS07M in Chinese cabbage. Plants were pre-drenched with these strains followed by antibiotics application at recommended and ten-fold diluted concentration to test the effect on biocontrol ability against soft rot caused by Pectobacterium carotovorum SCC1. The viability of the two biocontrol strains and bacterial pathogen SCC1 was significantly reduced by oxolinic acid and streptomycin in vitro assay, but not by validamycin A. In plant trials, strains EXTN-1 and BS07M controlled soft rot in Chinese cabbage, and there was a significant difference in disease severity when the antibiotics were applied to the plants drenched with the two biocontrol agents. Additional foliar applications of oxolinic acid and streptomycin reduced the disease irrespective of pre-drench treatment of the PGPRs. However, when the plants were pre-drenched with EXTN-1 followed by spray of validamycin A at recommended concentration, soft rot significantly reduced compared to untreated control. Similarly, strains EXTN-1 and BS07M significantly enhanced plant growth, but it did not show synergistic effect with additional spray of antibiotics. Populations of the EXTN-1 or BS07M in the rhizosphere of plants sprayed with antibiotics were significantly affected as compared to control. Taken together, our results suggest that the three antibiotics used for soft rot control in Chinese cabbage could affect bacterial mediated biocontrol and plant growth promoting activities. Therefore, combined treatment of the PGPRs and the commercial antibiotics should be carefully applied to sustain environmental friendly disease management.
 Keywords
Antibiotics;Biocontrol;Chinese cabbage;Plant growth promotion;Soft rot;
 Language
English
 Cited by
 References
1.
Ahemad, M. and Khan, M. S. 2012. Effect of fungicides on plant growth promoting activities of phosphate solubilizing Pseudomonas putida isolated from mustard (Brassica compestris) rhizosphere. Chemosphere 86: 945-950. crossref(new window)

2.
Bais, H. P., Weir, T. L., Perry, L. G., Gilroy, S. and Vivanco, J. M. 2006. The role of root exudates in rhizosphere interactions with plants and other organisms. Annu. Rev. Plant Biol. 57: 233-266. crossref(new window)

3.
Dutta, S. and Podile, A. R. 2010. Plant growth promoting rhizobacteria (PGPR): the bugs to debug the root zone. Crit. Rev. Microbiol. 36: 232-244. crossref(new window)

4.
Fukusawa, K., Sakurai, H., Shimizu, S., Naganawa, H. and Kondo, S. 1980. 3-Phosphoryldihydro-streptomycin produced by the inactivating enzyme of Erwinia carotovora. J. Antibiot. 33: 122-123. crossref(new window)

5.
Gerhardson, B. 2002. Biological substitutes for pesticides. Trends Biotechnol. 20: 338-343. crossref(new window)

6.
Huang, T. C. and Burr, T. J. 1990. Characterization of plasmids that encode streptomycin-resistance in bacterial epiphytes of apple. J. Appl. Microbiol. 86: 741-751.

7.
Hyun, J.-W., Kim, H.-J., Yi, P.-H., Hwang, R.-Y. and Park, E.-W. 2012. Mode of action of streptomycin resistance in the citrus canker pathogen (Xanthomonas smithii subsp. citri) in Jeju island. Plant Pathol. J. 28: 207-211. crossref(new window)

8.
Ishikawa, R., Suzuki-Nishimoto, M., Fukuchi, A. and Matsuura, K. 2004. Effective control of cabbage black rot by validamycin A and its effect on extracellular polysaccharide-production of Xanthomonas campestris pv.campestris. J. Pestic. Sci. 29: 209-213. crossref(new window)

9.
Jacobsen, B. J., Zidack, N. K. and Larson, B. J. 2004. The role of Bacillusbased biological control agents in integrated pest management systems: Plant diseases. Phytopathology 94: 1272-1275. crossref(new window)

10.
Kwon, H. R., Choi, G. J., Choi, Y. H., Jang, K. S., Sung, N.-D., Kang, M. S., Moon, Y., Lee, S. K. and Kim, J.-C. 2009. Suppression of pine wild disease by an antibacterial agent, oxolinic acid. Pest Manag. Sci. 66: 634-639.

11.
Levene, H. 1960. Contributions to Probability and Statistics: Essays in Honor of Harold Hotelling. Stanford University Press, Stanford, CA.

12.
Obradovic, A., Jones, J. B., Momol, M. T., Olson, S. M., Jackson, L. E., Balogh, B., Guven, K. and Iriarte, F. B. 2005. Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. Plant Dis. 89: 712-716. crossref(new window)

13.
Park, K. S., Diby, P., Kim, Y. K., Nam, K. W., Lee, Y. K., Choi, H. W. and Lee, S. Y. 2007. Induced systemic resistance by Bacillus vallismortis EXTN-1 suppressed bacterial wilt in tomato caused by Ralstonia solanacearum. Plant Pathol. J. 23: 22-25. crossref(new window)

14.
Park, K. S., Paul, D., Ryu, K. R., Kim, E. Y. and Kim, Y. K. 2006a. Bacillus vallismortis strain EXTN-1 mediated systemic resistance against Potato Virus X and Y (PVX & PVY) in the field. Plant Pathol. J. 22: 360-363. crossref(new window)

15.
Park, K. S., Paul, D. and Ye, W. H. 2006b. Bacillus vallismortis EXTN-1 mediated growth promotion and disease suppression in rice (Oryza sativa L.). Plant Pathol. J. 22: 278-282. crossref(new window)

16.
Park, J.-W., Balaraju, K., Kim, J.-W., Lee, S.-W. and Park, K. 2013. Systemic resistance and growth promotion of chili pepper induced by an antibiotic producing Bacillus vallismortis strain BS07. Biol. Control 65: 246-257. crossref(new window)

17.
Sang, M. K. and Kim, K. D. 2012. The volatile-producing Flavobacterium johnsoniae strain GSE09 shows biocontrol activity against Phytophthora capsici in pepper. J. Appl. Microbiol. 113: 383-398. crossref(new window)

18.
Sisler, H. D. 1986. Control of fungal diseases by compounds acting as antipenetrants. Crop Prot. 5: 306-313. crossref(new window)

19.
Smith, W. L. 1962. Chemical treatments to reduce postharvest spoilage of fruits and vegetables. Bot. Rev. 28: 411-445. crossref(new window)

20.
Smith, M. D., Hartnett, D. C. and Rice, C. W. 2000. Effects of longterm antibiotic applications on microbial properties in tallgrass prairie soil. Soil Biol. Biochem. 32: 935-946. crossref(new window)

21.
Snyder, M. and Drlica, K. 1979. DNA gyrase on the bacterial chromosome: DNA cleavage induced by oxolinic acid. J. Mol. Biol. 131: 287-302. crossref(new window)

22.
Patricia, S. M., Virginia, O. S., George, W. S. and Alan, L. J. 2002. Antibiotic use in plant agriculture. Annu. Rev. Phytopathol. 40: 443-465. crossref(new window)

23.
Ren, J. P., Petzoldt, R. and Dickson, M. H. 2001. Screening and identification of resistance to bacterial soft rot in Brassica rapa. Euphytica 118: 271-280. crossref(new window)

24.
Thanh, D. T., Tarn, L. T. T., Hanh, N. T., Tuyen, N. H., Bharathkumar, S., Lee, S. Y. and Park, K.-S. 2009. Biological control of soilborne diseases on tomato, potato and black pepper by selected PGPR in the greenhouse and field in Vietnam. Plant Pathol. J. 25: 264-269.

25.
Vanjildorj, E., Song, S. Y., Yang, Z. H., Choi, J. E., Noh, Y. S., Park, S., Lim, W. J., Cho, K. M., Yun, H. D. and Lim, Y. P. 2009. Enhancement of tolerance to soft rot disease in the transgenic Chinese cabbage (Brassica rapa L. spp. pekinensis) inbred line, Kenshin. Plant Cell Rep. 28: 1581-1591. crossref(new window)

26.
Whipps, J. M. 2001. Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 52: 487-511. crossref(new window)

27.
Wang, Z.-J., Ji, S., Si, Y.-X., Yang, J.-M., Qian, G.-Y., Lee, J. and Yin, S.-J. 2013. The effect of validamycin A on tyrosinase: Inhibition kinetics and computational simulation. Int. J. Biol. Macromol. 55: 15-23. crossref(new window)

28.
Yang, C., Hamel, C., Gan, Y. and Vujanovic, V. 2012. Tag-encoded pyrosequencing analysis of the effects of antibiotic application and plant genotype on rhizobacterial communities. Appl. Soil Ecol. 60: 92-97. crossref(new window)