Advanced SearchSearch Tips
Development of an Efficient Screening System for Resistance of Tomato Cultivars to Ralstonia solanacearum
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Research in Plant Disease
  • Volume 21, Issue 4,  2015, pp.290-296
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.4.290
 Title & Authors
Development of an Efficient Screening System for Resistance of Tomato Cultivars to Ralstonia solanacearum
Lee, Ji Hyun; Jang, Kyoung Soo; Choi, Yong Ho; Kim, Jin-Cheol; Choi, Gyung Ja;
  PDF(new window)
This study was conducted to establish an efficient screening system for resistant tomato to bacterial wilt caused by Ralstonia solanacearum. Under several conditions such as inoculation methods, growth stages of tomato seedlings, inoculum concentrations, and incubating temperatures after inoculation, development of bacterial wilt on nine resistant or susceptible cultivars of tomato was investigated. To inoculate by drenching the non-cut roots with the bacterial suspension was better to distinguish resistance and susceptibility of tomato cultivars than by drenching the cut roots using scalpel. And 'Hawaii7996' a resistant tomato to R. solanacearum showed high resistance at all the tested conditions including growth stages (3-, 6-, 8-, 10-leaf stages), inoculum concentrations () and incubation temperatures (25, 30, ). On the other hands, susceptible cultivars represented disease index of 3.7 and 3.9 at 6- and 8-leaf stages, respectively. At 3- and 10-leaf stages, the cultivars demonstrated lower disease severity of 2.1 and 0.5, respectively, than at 6- and 8-leaf stages. When the inoculated seedlings were incubated in growth chambers of 25, 30 and , disease severity of susceptible cultivars was significantly greater at 30 and than at . In addition, the level of resistance of the tomato cultivars was not significantly affected by inoculum concentrations of . On the basis of the results, we suggest an efficient screening method to measure resistance level of tomato cultivars to bacterial wilt. The eight-leaf stage seedlings transplanted 7 days before inoculation, are inoculated with R. solanacearum by drenching the non-cut roots with a bacterial suspensions () to give inoculum volume of 50 ml/soil l. The inoculated plants are incubated in a growth room at for 12-13 days with 12-hour light a day.
Bacterial wilt;Breeding;Disease resistance;Screening;Solanum lycopersicum;
 Cited by
Acosta, J. C., Gilbert, J. C. and Quinon, V. L. 1964. Heritability of bacterial wilt resistance in tomato. P. Am. Soc. Hortic. Sci. 84: 455-462.

Anand, N., Sadashiva, A. T., Tikoo, S. K., Ramkishun, M. and Reddy, K. 1993. Resistance to bacterial wilt in tomato: gene dosage effects. In: Bacterial Wilt, eds. by G. L. Hartman and A. C. Hayward, pp. 142-148. Hayward ACIAR Proc. 45, Kaoshiung, Taiwan.

Anon. 1975. The Asian Vegetable Research and Development Center. Tomato Report for 1975. pp. 25-28.

Buddenhagen, I. and Kelman, A. 1964. Biological and physiological aspects of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 2: 203-230. crossref(new window)

Buddenhagen, I., Sequeira, L. and Kelman, A. 1962. Designation of races in Pseudomonas solanacearum. Phytopathology 52: 726.

Carmeille, A., Caranta, C., Dintinger, J., Prior, P., Luisetti, J. and Besse, P. 2006. Identification of QTLs for Ralstonia solanacearum race 3-phylotype II resistance in tomato. Theor. Appl. Genet. 113: 110-121. crossref(new window)

Danesh, D., Aarons. S., McGill, G. E. and Young, N. D. 1994. Genetic dissection of oligogenic resistance to bacterial wilt in tomato. Mol. Plant-Microbe Interact. 7: 464-471. crossref(new window)

Grimault, V., Gelie, B., Lamattre, M., Prior, P. and Schimidt, J. 1994. Comparative histology of resistant and susceptible tomato cultivars infected by Pseudomonas solanacearum. Physiol. Mol. Pathol. 44: 105-123. crossref(new window)

Han, Y., Min, J., Park, J., Han, K., Kim, D., Lee, J. and Kim, H. 2009. Screening of tomato cultivars resistant to bacterial wilts. Res. Plant Dis. 15: 198-201. (In Korean) crossref(new window)

Han, Y.-K., Han, K.-S., Lee, S.-C. and Kim, S. 2011. Control of bacterial wilt of tomato using copper hydroxide. Korean J. Pestic. Sci. 15: 298-302. (In Korean)

Hayward, A. C. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29: 6587.

He, L. Y., Sequeira, L. and Kelman, A. 1983. Characteristics of strains of Pseudomonas solanacearum from China. Plant Dis. 67: 1357-1361. crossref(new window)

Jeong, Y., Kim, J., Kang, Y., Lee, S. and Hwang, I. 2007. Genetic diversity and distribution of Korea isolates of Ralstonia solanacearum. Plant Dis. 91: 1277-1287. crossref(new window)

Kelman, A. and Sequeira, L. 1965. Root to root spread of Pseudomonas solanacearum. Phytopathology 55: 304-309.

Krausz, J. P. and Thurston, H. D. 1975. Breakdown of resistance to Ralstonia solanacearum in tomato. Phytopathology 34: 443-458.

Lee, H. J., Jo, E. J., Kim, N. H., Chae, Y. and Lee, S. W. 2011. Disease responses of tomato pure lines against Ralstonia solanacearum strains from Korea and susceptibility at high temperature. Res. Plant Dis. 17: 326-333. (In Korean) crossref(new window)

Mew, T. W. and Ho, W. C. 1976. Varietal resistance to bacterial wilt in tomato. Plant Dis. Rep. 60: 264-268.

Monma, S. and Sakata, Y. 1993. Inheritance of resistance to bacterial wilt in tomato. In: Bacterial Wilt, eds. by G. L. Hartman and A. C. Hayward, pp. 149-153. Hayward ACIAR Proc. 45. Kaoshiung, Taiwan.

Nakaho, K. and Takaya, S. 1993. Resistance of tomato rootstock cultivars to Pseudomonas solanacearum evaluated by infection rate under different testing conditions. In: Bacterial Wilt, eds. by G. L. Hartman and A. C. Hayward, pp. 138-141. Hayward ACIAR Proc. 45, Kaoshiung, Taiwan.

Park, E. J., Lee, S. D., Chung, E. J., Lee, M. H., Um, H. Y., Murugaiyan, S., Moon, B. J. and Lee, S.-W. 2007. MicroTom-A model plant system to study bacterial wilt by Ralstonia solanacearum. Plant Pathol. J. 23: 239-244. crossref(new window)

Roberts, P. D., Denny, T. P. and Schell, M. A. 1988. Cloning of the egl gene of Pseudomonas solanacearum and analysis of its role in phytopathogenicity. J. Bacteriol. 170: 1445-1451.

SAS Institute Inc. 1989. SAS/STAT user' guide, version 6.4th ed. SAS Institute Inc., Gary, N. C.

Sceelathakumary, L. A. and Peter, K. V. 1984. Inheritance of combined wilt resistance in tomato. Tomato Genet. Coop. Rep. 34: 16.

Schaad, N. W., Jones, J. B. and Chun, W. 2001. Laboratory, Guide for Identification of Plant Pathogenic Bacteria. 3rd ed. APS Press, St. Paul, USA.

Schmit, J. 1978. Microscopic study of early stages of infection by Pseudomonas solanacearum E. F. S. on "in vitro" grown tomato seedlings. Proc. 4th Int. Conf. Plant Pathol. Bacteriol. pp. 841-856.

Seo, S. T., Park, J. H., Han, K. S., Cheong, S. R. and Lee, S. 2007. Genetic diversity of Ralstonia solanacearum strain isolated from pepper and tomato plants in Korea. Res. Plant Dis. 13: 24-29. (In Korean) crossref(new window)

Singh, K. 1961. Inheritance of North Carolina type of bacterial wilt resistance in tomato Lycopersicon esculentum L.M.S. Thesis. University of Hawaii, Honolulu.

Thoquet, P., Olivier, J., Sperisen, C., Rogowsky, P., Laterrot, H. and Grimsley, N. 1996a. Quantitative trait loci determining resistance to bacterial wilt in tomato cultivar Hawaii7996. Mol. Plant-Microbe Interact. 9: 826-836. crossref(new window)

Thoquet, P., Olivier, J., Sperisen, C., Rogowsky, P., Prior, P., Anais, G., Mangin, B., Bazin, B., Nazer, R. and Grimsley, N. 1996b. Polygenic resistance of tomato plants to bacterial wilt in the French West Indies. Mol. Plant-Microbe Interact. 9: 837-842. crossref(new window)

Vasse, J., Frey, P. and Trigalet, A. 1995. Microscopic studies of intercellular infection and protoxylem invasion of tomato roots by Pseudomonas solanacearum. Mol. Plant-Microbe Interact. 8: 241-251. crossref(new window)

Walker, J. M. 1967. Hereditary resistance to disease in Pseudomonas solanacearum. Phytopathology 42: 628-634.

Wang, J.-F., Olivier, J., Thoquet, P., Mangin, B., Sauviac, L. and Grimsley, N. H. 2000. Resistance of tomato line Hawaii7996 to Ralstonia solanacearum Pss4 in Taiwan is controlled mainly by a major strainspecific locus. Mol. Plant-Microbe Interact. 13: 6-13. crossref(new window)

Winstead, N. N. and Kelman, A. 1952. Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology 42: 628-634.