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Detection of Soybean mosaic virus by Reverse Transcription Loop-mediated Isothermal Amplification
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  • Journal title : Research in Plant Disease
  • Volume 21, Issue 4,  2015, pp.315-320
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.4.315
 Title & Authors
Detection of Soybean mosaic virus by Reverse Transcription Loop-mediated Isothermal Amplification
Lee, Yeong-Hoon; Bae, Dae-Hyeon; Kim, Bong-Sub; Yoon, Young-Nam; Bae, Soon-Do; Kim, Hyun-Joo; Mainali, Bishwo P.; Park, In-Hee; Lee, Su-Heon; Kang, Hang-Won;
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Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I under daylight and UV light. Optimal reaction condition was at for 60 min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.
 Cited by
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