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Simple and Rapid Detection for Rice stripe virus Using RT-PCR and Porous Ceramic Cubes
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  • Journal title : Research in Plant Disease
  • Volume 21, Issue 4,  2015, pp.321-325
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.4.321
 Title & Authors
Simple and Rapid Detection for Rice stripe virus Using RT-PCR and Porous Ceramic Cubes
Hong, Su-Bin; Kwak, Hae-Ryun; Kim, Mi-Kyeong; Seo, Jang-Kyun; Shin, Jun-Sung; Han, Jung-Heon; Kim, Jeong-Soo; Choi, Hong-Soo;
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 Abstract
A rapid and simple RT-PCR diagnosis method for detection of Rice stripe virus (RSV), one of major virus infecting rice, was developed using porous ceramic cubes in this study. The porous ceramic cube can rapidly absorb biological molecules such as small-sized proteins and nucleic acid fragments into its pores. We examined whether this ability of porous ceramic cubes could be applied for isolating viral nucleic acids or particles from the RSV- infected plant tissues. In this study, we found that the porous ceramic cube was capable of absorbing a detection level of viruses from the rice tissues infected with RSV and established RT-PCR-based RNA diagnosis method using porous ceramic cubes.
 Keywords
Porous ceramic cube;RSV;RT-PCR;Virus diagnosis;
 Language
Korean
 Cited by
 References
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