Advanced SearchSearch Tips
Simple and Rapid Detection for Rice stripe virus Using RT-PCR and Porous Ceramic Cubes
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Research in Plant Disease
  • Volume 21, Issue 4,  2015, pp.321-325
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.4.321
 Title & Authors
Simple and Rapid Detection for Rice stripe virus Using RT-PCR and Porous Ceramic Cubes
Hong, Su-Bin; Kwak, Hae-Ryun; Kim, Mi-Kyeong; Seo, Jang-Kyun; Shin, Jun-Sung; Han, Jung-Heon; Kim, Jeong-Soo; Choi, Hong-Soo;
  PDF(new window)
A rapid and simple RT-PCR diagnosis method for detection of Rice stripe virus (RSV), one of major virus infecting rice, was developed using porous ceramic cubes in this study. The porous ceramic cube can rapidly absorb biological molecules such as small-sized proteins and nucleic acid fragments into its pores. We examined whether this ability of porous ceramic cubes could be applied for isolating viral nucleic acids or particles from the RSV- infected plant tissues. In this study, we found that the porous ceramic cube was capable of absorbing a detection level of viruses from the rice tissues infected with RSV and established RT-PCR-based RNA diagnosis method using porous ceramic cubes.
Porous ceramic cube;RSV;RT-PCR;Virus diagnosis;
 Cited by
Hosokawa, M., Matsushita, Y., Uchida, H. and Yazawa, S. 2006. Direct RT-PCR method for detecting two chrysanthemum viroids using minimal amounts of plant tissue. J. Virol. Methods 131: 28-33. crossref(new window)

Kim, J. S., Lee, S. H., Choi, H. S., Choi, G. S., Cho, J. D. and Chung, B. N. 2008. Survey of viral diseases occurrence on major crops in 2007. Res. Plant Dis. 14: 1-9. (In Korean) crossref(new window)

Kim, J. S., Lee, S. H., Choi, H. S., Kim, M. K., Kwak, H. R., Cho, J. D., Choi, G. S. and Kim, J. Y. 2009a. Occurrence of virus diseases on major crops in 2008. Res. Plant Dis. 15: 1-7. (In Korean) crossref(new window)

Kim, J. S., Lee, S. H., Choi, H. S., Cho, J. D., Noh, T. W. and Kim, J. Y. 2009b. Convenient genetic diagnosis of virion captured (VC)/RT-PCR for rice viruses (RSV, RBSDV) and small brown plant hopper. Res. Plant Dis. 15: 57-62. (In Korean) crossref(new window)

Nolasco, G., De Blas, C., Torres, V. and Ponz, F. 1993. A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. J. Virol. Methods 45: 201-218. crossref(new window)

Shin, J. S., Kim, H. J., Choi, H. S. and Kim, J. S. 2015. A potential use of porous ceramic matters for simple preparation of DNA and RNA to amplify a target sequence of cucumber mosaic virus and plant DNA. The KSPP, p178. (Abstract)