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Rapid Detection Method for Fusaric Acid-producing Species of Fusarium by PCR
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  • Journal title : Research in Plant Disease
  • Volume 21, Issue 4,  2015, pp.326-329
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.4.326
 Title & Authors
Rapid Detection Method for Fusaric Acid-producing Species of Fusarium by PCR
Lee, Theresa; Kim, Sosoo; Busman, Mark; Proctor, Robert H.; Ham, Hyeonhui; Lee, Soohyung; Hong, Sung Kee; Ryu, Jae-Gee;
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 Abstract
Fusaric acid is a mycotoxin produced by species of the fungus Fusarium and can act synergistically with other Fusarium toxins. In order to develop a specific detection method for fusaric acid-producing fungus, PCR primers were designed to amplify FUB10, a transcription factor gene in fusaric acid biosynthetic gene cluster. When PCR with Fub10-f and Fub10-r was performed, a single band (~550 bp) was amplified from F. oxysporum, F. proliferatum, F. verticillioides, F. anthophilum, F. bulbicola, F. circinatum, F. fujikuroi, F. redolens, F. sacchari, F. subglutinans, and F. thapsinum, all of which were known for fusaric acid production. Whereas the FUB10 specific band was not amplified from Fusarium species known to be trichothecene producer. Because production of fusaric acid can co-occur in species that also produce fumonisin mycotoxins, we developed a multiplex PCR assay using the FUB10 primers as well as primers for the fumonisin biosynthetic gene FUM1. The assay yielded amplicons from fumonisin producers such as F. proliferatum and F. verticillioides, allowing for the simultaneous detection of species with the genetic potential to produce both types of mycotoxins.
 Keywords
Detection;Fusaric acid;Fusarium;Primer;
 Language
Korean
 Cited by
 References
1.
Bacon, C. W., Porter, J. K. and Norred, W. P. 1995. Toxic interaction of fumonisin B1 and fusaric acid measured by injection into fertile chicken egg. Mycopathologia 129: 29-35. crossref(new window)

2.
Bacon, C. W., Porter, J. K., Norred, W. P. and Leslie, J. F. 1996. Production of fusaric acid by Fusarium species. Appl. Environ. Microbiol. 62: 4039-4043.

3.
Brown, D. W., Butchko, R. A. E., Busman, M. and Proctor, R. H. 2012. Identification of gene clusters associated with fusaric acid, fusarin, and perithecial pigment production in Fusarium verticillioides. Fungal Genet. Biol. 49: 521-532. crossref(new window)

4.
Brown, D. W., Lee, S.-H., Kim, L.-H., Ryu, J.-G., Lee, S., Seo, Y., Kim, Y. H., Busman, M., Yun, S.-H., Proctor, R. H. and Lee, T. 2015. Identification of a 12-gene fusaric acid biosynthetic gene cluster in Fusarium species through comparative and functional genomics. Mol. Plant-Microbe Interact. 28: 319-332. crossref(new window)

5.
Desjardins, A. E. 2006. Fusarium mycotoxins: chemistry, genetics, and biology. The American Phytopathological Society, St. Paul, Minnesota, U.S.A. 260 pp.

6.
Gaumann, E. 1957. Fusaric acid as a wilt toxin. Phytopathology 47: 342-357.

7.
Liu, J., Bell, A. A., Stipanovic, R., Puckhaber, L. and Shim, W. 2011a. A polyketide synthase gene and an aspartate kinase like gene are required for the biosynthesis of fusaric acid in Fusarium f. sp. vasinfectum In Proceedings of the Beltwide Cotton Conferences. eds. by S. Boyd, M. Huffman and B. Robertson. Memphis, TN.

8.
Liu, J., Bell, A. A., Wheeler, M. H., Stipanovic, R. D. and Puckhaber, L. S. 2011b. Phylogeny and pathogenicity of Fusarium oxysporum isolates from cottonseed imported from Australia into California for dairy cattle feed. Can. J. Microbiol. 57: 874-886. crossref(new window)

9.
Niehaus, E. M., von Bargen, K. W., Espino, J. J., Pfannmuller, A., Humpf, H. U. and Tudzynski, B. 2014. Characterization of the fusaric acid gene cluster in Fusarium fujikuroi. Appl. Microbiol. Biotechnol. 98: 1749-1762. crossref(new window)

10.
Proctor, R. H., Plattner, R. D., Brown, D. W., Seo, J.-A. and Lee, Y.-W. 2004. Discontinuous distribution of fumonisin biosynthetic genes in the Gibberella fujikuroi species complex. Mycol. Res. 108: 815-822. crossref(new window)