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Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea
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  • Journal title : Research in Plant Disease
  • Volume 21, Issue 4,  2015, pp.346-350
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2015.21.4.346
 Title & Authors
Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea
Cho, Kang Hee; Kim, In-Soo; Kil, Eui-Joon; Park, Seo Jun; Kim, Se Hee; Choi, In-Myung; Kim, Dae-Hyun;
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To identify genome sequences of Apple scar skin viroid (ASSVd) isolates in Korea, the field survey was performed from `Hongro` apple orchards located in eight sites in South Korea (Bongwha, Cheongsong, Dangjin, Gimchoen, Muju, Mungyeong, Suwon, and Yeongwol). ASSVd was detected by RT-PCR and PCR fragments were cloned into cloning vector. Full-length viral genomes of eight ASSVd isolates were sequenced and compared with 21 isolates reported previously from Korea, India, China, Japan and Greece. Eight isolates in this study showed 92.2-99.7% nucleotide sequence identities with those reported previously. Phylogenetic analysis showed that seven isolates reported in this study belong to the same group distinct from other groups.
Apple scar skin viroid;Phylogenetic analysis;Sequencing;
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