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Screening Methods for Resistant Cucumber Cultivars against Cucumber Scab Caused by Cladosporium cucumerinum Using Cucumber Fruits and Seedlings
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  • Journal title : Research in Plant Disease
  • Volume 22, Issue 1,  2016, pp.18-24
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/RPD.2016.22.1.18
 Title & Authors
Screening Methods for Resistant Cucumber Cultivars against Cucumber Scab Caused by Cladosporium cucumerinum Using Cucumber Fruits and Seedlings
Park, So-Hyang; Hong, Sung-Jun; Shim, Chang-Ki; Kim, Min-Jeong; Park, Jong-Ho; Han, Eun-Jung; Park, Jong-Won; Jee, Hyeong-Jin; Kim, Seok-Cheol; Kim, Yong-Ki;
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This study was conducted to elucidate the cultural and pathogenic characteristics of Cladosporium cucumerinum PT1 and resistance of 81 commercial cucumbers (Cucumis sativus). Cucumber leaves and fruits appeared as scab were collected from a plastic film house located in Pyeongtaek, Gyeonggi-province, Korea in late March, 2015. A casual fungus was isolated from the diseased fruits on potato dextrose agar and it was identified as C. cucumerinum PT1 based on the morphological characteristics. To find out the effect of wounding and fruit size on the development of cucumber scab, small (<10 cm long), medium (10 to 20 cm long), and large (>20 cm long, commercially mature fruit) size cucumber fruits were harvested, C. cucumerinum PT1 pathogens were inoculated with a single droplet of suspension ( spores/ml) on wounded or unwounded cucumber fruits. Small fruits were completely damaged with showing severe water-soaking symptoms and fast pathogen growth regardless of wounded or unwounded. Meanwhile slight water-soaking symptoms on medium and large size fruits occurred and disease development into plant tissues was observed only on wounded fruits. Disease resistance of 81 commercial cucumber cultivars was evaluated on third-stage seedlings and small fruits by inoculating suspension ( spores/ml) of C. cucumerinum PT1. As a result, mini and pickling cultivar groups were resistant, `Cheoeumcheoreom` cultivar was symptomless and the other cultivars were resistant to medium resistant. On the other hand, most of cucumber cultivars belonging to the other groups were susceptible. Disease resistance of cucumber against cucumber scab was significantly different among cultivars and a few cucumber cultivars showed different disease resistant responses to two bioassay methods using seedlings and small fruits. Therefore, to screen scab resistance in cucumber, a test using both fruits and seedlings is advisable. We think that the selected resistant cultivars can be used to control cucumber scab effectively under the farmhouse condition.
Cladosporium cucumerinum;Cucumber;Fruit;Scab;Seedling;
 Cited by
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