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Insulin-like Growth Factor-I Induces FABPpm Expression in C2C12 Myotubes
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 10,  2015, pp.1098-1102
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.10.1098
 Title & Authors
Insulin-like Growth Factor-I Induces FABPpm Expression in C2C12 Myotubes
Kim, Hye Jin; Yoon, Hae Min; Lee, Won Jun;
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FABPpm (plasma membrane-bound fatty acid binding protein ) is highly expressed in skeletal muscle. The principal role of this protein is modulating fatty acid uptake and metabolism. The influence of insulin-like growth factor-I (IGF-I), which is a major regulator of skeletal muscle cells, on FABPpm in skeletal muscle cells has not been investigated. To determine the effect of IGF-I on the expression of FABPpm, differentiated C2C12 murine skeletal muscle cells were treated with 20 ng/ml of IGF-I for different times. IGF-I increased the expression of FABPpm in a time-dependent manner. The mRNA level of FABPpm was measured by real-time quantitative PCR to determine whether the IGF-1-induced induction of FABPpm was regulated pretranslationally. The IGF-I treatment resulted in very rapid induction of the FABPpm mRNA transcript in the C2C12 myotubes. After 24 and 48 hr of the IGF-I treatment, FABPpm mRNA increased 130 and 179%, respectively. The increase in the protein expression returned to control levels after 72 hr of the IGF-I treatment, suggesting that IGF-1 regulated the FABPpm gene pretranslationally in skeletal muscle cells. This is the first evidence that IGF-I has a modulatory effect on the expression of FABPpm. In conclusion, IGF-I induced rapid transcriptional modification of the FABPpm gene in C2C12 skeletal muscle cells and exerted modulatory effects on FABPpm.
C2C12 myotube;FABPpm;fatty acids;IGF-I;lipid metabolism;
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