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Microbiological and Chemical Changes of Complete Feed during Spoilage
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 10,  2015, pp.1148-1155
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.10.1148
 Title & Authors
Microbiological and Chemical Changes of Complete Feed during Spoilage
Yi, Kwonjung; Yeon, Jae-Sung; Kim, Juhyeon; Kim, Sam Churl; Moon, Hyung-In; Jeon, Che Ok; Lee, Sang Suk; Kim, Dong-Woon; Kim, Soo-Ki;
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 Abstract
Commercial complete feeds contain enough nutrients to support animal growth and it is easy to be spoiled under proper temperature and humid conditions. The aim of this study was to investigate microbiological and chemical changes on complete feed for milking cow under open-air exposure with moisture 33% at 30℃ during 15 days. pH decreased 6.29 to 4.66 and water activity decreased gradually 0.99 to 0.95. Bacteria increased 6.2×106~1.6×107 to 2.1×109 CFU/g at 5 days and showed 108 CFU/g until 15 days. Fungi increased 103 CFU/g to 8.0×104 CFU/g. During the processing of spoilage, bacteria such as Acinetobacter oleivorans, Pediococcus acidilactici, Acinetobacter oleivorans, Weissella cibaria, and Methylobacterium komagatae were identified and fungi such as Fusarium sp. and Mucor sp. were also identified. Moisture content increased until 10 days (p<0.01). Crude protein was not changed so much whereas crude fat decreased 6.0% to 5.5% (p<0.01). Crude fiber and crude ash changed 2.0~ 3.0% and 4.5~ 4.8% levels with no significance, respectively. Gross energy was not almost changed at 4,400 kcal/g. During spoilage, lactate and propionate increased whereas acetate was not detected. Protease and lipase activities increased significantly during spoilage (p<0.01). Zearalenone content increased 59.2 μg/kg to 623.8 μg/kg, showing 10.5 times more production. During feed spoilage, pH decreased with microbial growth and various chemical changes were occurred.
 Keywords
Feed;microorganism;monitoring;spoilage;zearalenone;
 Language
Korean
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