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Growth Characteristics of a Pyruvate Decarboxylase Mutant Strain of Zymomonas mobilis
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 11,  2015, pp.1290-1297
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.11.1290
 Title & Authors
Growth Characteristics of a Pyruvate Decarboxylase Mutant Strain of Zymomonas mobilis
Xun, Zhao; Peter L., Rogers; Kwon, Eilhann E.; Jeong, Sang Chul; Jeon, Young Jae;
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 Abstract
Studies of the inactivation of a gene encoding pyruvate decarboxylase, pdc, in an ethanol-producing bacterium, Zymomonas mobilis, identified a mutant strain with 50% reduced PDC activity. To evaluate the possibility of a carbon-flux shift from an ethanol pathway toward higher value fermentation products, including pyruvate, succinate, and lactate, fermentation studies were carried out. Despite attempts to silence pdc expression in the wild-type strain ZM4 using cat-inserted pdc and pdc-deleted homologs by electroporation, the strain isolated showed partial gene activation. Fermentation experiments with the PDC mutant strain showed that the reduced expression level of PDC activity resulted in decreased rates of substrate uptake and ethanol production, together with increased pyruvate accumulation of 2.5 g l-1 , although lactate and succinate concentrations were not significantly enhanced in these modified strains. Despite numerous attempts, no strains were isolated in which complete pdc inactivation occurred. This result indicates that the ethanol fermentation pathway of this bacterium is totally dependent on the activity of the PDC enzyme. To ensure a redox balance of intracellular NAD and NADH levels, other enzymes, such as lactate dehydrogenase for lactate, and enzymes involved in the production of succinic acid, such as pyruvate dehydrogenase (PDH) and malic enzymes, may be needed for their increased end-product production.
 Keywords
Fermentation;gene inactivation;higher value products;pyruvate decarboxylase;Zymomonas mobilis;
 Language
Korean
 Cited by
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