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Evaluation of Achyranthes japonica Ethanol Extraction on the Inhibition Effect of Hyluronidase and Lipoxygenase
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 12,  2015, pp.1370-1376
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.12.1370
 Title & Authors
Evaluation of Achyranthes japonica Ethanol Extraction on the Inhibition Effect of Hyluronidase and Lipoxygenase
Cho, Kyung-Soon;
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The 1, 1- diphenyl 2-picrylhyorazyl (DPPH) is a well-known radical and a trap (scavenger) for other radicals. Hyaluronidase (HAase) is an enzyme that depolymerizes the polysaccharide hyaluronic acid (HA) in the extracellular matrix of connective tissue. Lipoxygenase (LOX) enzyme was reported to convert the arachidonic, linoleic and other polyunsaturated fatty acid into biologically active metabolites involved in the inflammatory and immune responses. The purpose of the present study is to evaluate plant extracts as sources of natural antioxidants and to examine whether Achyranthes japonica having significant DPPH, HAase and LOX inhibitory activity. The inhibitory effect of HAase by A. japonica was assayed using a Morgan microplate assay. The antioxidant activity of the A. japonica extracts was measured on the basis of the scavenging activity of the stable 1, 1- diphenyl 2-picrylhyorazyl (DPPH) free radical. DPPH scavenging activity of matured roots of A. japonica was evaluated at 4.0 mg/ml was 87.8% and that of young roots was 86.2% at same concentration. The roots of A. japonica showed maximum inhibition of HAase activity (IC50 = 27.7 μg/ml). The highest LOX inhibition was recorded in the root extract among three vegetative parts. Inhibition of HAase activity of roots may contribute towards the development of herbal medicines. Although percent inhibition of lipoxygenase by Achyranthes japonica for all young and matured groups for leaves, stems, and roots at different concentrations, there were not show a statistically significant difference (p<0.05).
1;1- diphenyl 2-picrylhyorazyl (DPPH);Achyranthes japonica;hyaluronidase;lipoxygenase;
 Cited by
Akular, U. S. and Odhav, B. 2008. In vitro 5-Lipoxygenase inhibition of polyphenolic antioxidents from undomesticated plants of South Africa. J. Med. Plants Res. 2, 207-212.

Amit, S., Saraswati1, B., Kamalesh, U. and Kumud, U. 2013. Formulation and evaluation of a novel herbal gel of Equisetum arvense extract. J. Pharm. Phytochem. 1, 80-86.

Azila, Y. and Don, M. M. 2012. Evaluation of Trametes lactinea extracts on the inhibition of hyaluronidase, lipoxygenase and xanthine oxidase. J. Phys. Sci. 23, 1-15.

Brand-Williams, W., Cuvelier, M. E. and Berset, C. 1995. Use of free radical method to evaluate antioxidant activity. Food Sci. Technol. 28, 25-30.

Chen, C. H., Chan, H. C., Chu, Y. T., Ho, H. Y., Chen, P. Y., Lee, T. H. and Lee, C. K. 2009. Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase and tyrosinase. Molecules 14, 2947-2958. crossref(new window)

Duthie, E. S. and Chain, E. A. 1939. A mucolytic enzyme in testes extract. Nature 144, 977-978.

Girish, K. S. and Kemparaju, K. 2005. Inhibition of Naja naja venom hyaluronidase by plant derived bioactive components and polysaccharides. Biochemistry (Mosc) 70, 948-952. crossref(new window)

Hwang, S. G., Yang, A., Kim, S. J., Kim, M. K., Kim, S. S., Oh, H. J., Lee, J. D., Lee, E. J., Nam, K. W. and Han, M. D. 2014. Screening of hyaluronidase inhibitor in Korean medicinal plants. J. Life Sci. 24, 498-504. crossref(new window)

Jung, S. M., Choi, S. I., Park, S. M. and Heo, T. R. 2007. Antimicrobial effect of Achyranthes japonica Nakai extracts against Clostridium difficile. Kor. J. Food Sci. Technol. 39, 564-568.

Kedare, S. B. and Singh, R. P. 2011. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Technol. 48, 412-422. crossref(new window)

Kumar, V. and Parmar, N. S. 2003. Herbs: A potential source for the development of new pytomedicinals. The Pharma. Review 1, 59-63.

Kwon, J. W., Lee, E. J., Kim, Y. C., Lee, H. S. and Kwon, T. O. 2008. Screening of antioxidant activity from medicinal plant extracts. Kor. J. Pharmacogn. 39, 155-163.

Ladd, D. and Thomas, J. R. 2015. Ecological checklist of the Missouri flora for floristic quality assessment. Phytoneuron 12, 1-274.

Lee, S. K., Zakaria, H. M., Cheng, H. S., Luyengi, L., Gamez, E. J. C., Mehta, R., Kinghorn A. D. and Pezzuto, J. M. 1998. Evaluation of the antioxidant potential of natural products. Combinat. Chem. High Througput Screen 1, 35-46.

Maity, N., Nema, N. K., Abedy, M. K., Sarkar, B. K. and Mukherjee, P. K. 2011. Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity. J. Ethnopharmacol. 137, 1300-1305. crossref(new window)

Mansuy, D., Cucurou, C., Biatry, B. and Battioni, J. P. 1988. Soybean lipoxygenase-catalyzed oxidations by linoleic acid hydroperoxide: Different reducing substrates and dehydrogenation of phenidone and BW755C. Biochem. Biophys. Res. Commun. 151, 339-346. crossref(new window)

Marcone, M. F., Jahaniaval, F., Aliee, H. and Kakuda, Y. 2003. Chemical characterization of Achyranthes bidentata seed. Food Chem. 81, 7-12. crossref(new window)

Menzel, E. J. and Farr, C. 1998. Hyaluronidase and its substrate hyaluronan: biochemistry, biological activities and therapeutic uses. Cancer Lett. 131, 3-11. crossref(new window)

Medley, M. E., H., Bryan, H., MacGregor, J. and John. W. Thieret. 1985. Achyranthes japonica (Miq.) Nakai (Amaranthaceae) in Kentucky and West Virginia: new to North America. Sida 11, 92-95.

Nema, N. K., Maity, N., Sarkar, B. and Mukherjee, P. K. 2011. Cucumis sativus fruit-potential antioxidant, anti-hyaluronidase, and anti-elastase agent. Arch. Dermatol. Res. 303, 247-252. crossref(new window)

Nuhn, P., Bilge, A., K6hler, T., Lettau, H. and Schneider, R. 1991. Trends bei der entwicklung yon lipoxygenasehemmern. Pharmazie 46, 81-88.

Oyetayo, V. O. 2008. Microbial load and antimicrobial property of two Nigerian herbal remedies. Afr. J. Trad. CAM. 5, 74-78.

Park, J. H., Kang, S. N., Shin, D., Hur, I. C., Kim, I. S. and Jin, S. K. 2013. Antioxidant activities of Achyranthes japonica Nakai extract and its application to the pork sausages. Asian-Australas J. Anim. Sci. 26, 287-294. crossref(new window)

Park, H. J., Lee, J. S., Hong, M. S., Kim, C. J., Kim, J. W., Lee, H. J. and Lim, S. 2004. The anti-nociceptive and anti-inflammatory effect of Achyranthes japonica Nakai. Kor. J. Orient. Med. 25, 8-14.

Piwowarski, J. P., Kiss, A. K. and Kozłowska-Wojciechowska, M. 2011. Anti-hyaluronidase and anti-elastase activity screening of tannin-rich plant materials used in traditional Polish medicine for external treatment of diseases with inflammatory background. J. Ethnopharmacol. 137, 937-941. crossref(new window)

Pogrel, M. A., Low, P. M. and Stern, R. 2003. Hyaluronan (hyaluronic acid) and its regulation in human saliva by hyaluronidase and its inhibitors. J. Oral Sci. 45, 85-91. crossref(new window)

Son, K. H., Hwang, J. H., Lee, S. H., Park, J. H., Kang, S. J., Chang, S. Y. and Lee, K. S.1999. Isolation and quantitative determination of 20-hydroxyecdysone from Achyranthis radix. Kor. J. Pharmacogn. 30, 335-339.

Sumantran, V. N., Kulkarni, A., Boddul, S., Chinchwade, T., Harsulkar, A., Patwardhan, B., Chopra, A. and Wagh, U. V. 2007. Chondroprotective potential of root extracts of Withania somnifera in osteoarthritis. J. Biosci. 32, 299-307. crossref(new window)

Wichtl, M. 1994. Herbal Drug and Phytopharmaceuticals, pp. 188-191, Medpharm Scientific Publishers: Stuttgart, Germany.