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A Potent Tissue Destructive Activity of Secreted Proteins of Aeromonas hydrophila
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 2,  2015, pp.214-222
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.2.214
 Title & Authors
A Potent Tissue Destructive Activity of Secreted Proteins of Aeromonas hydrophila
Kim, Kyu Lee; Choe, Yunjeong; Kang, Ho Young;
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 Abstract
Aeromonas hydrophila is the most common water fish pathogen and cause diseases such as hemorrhagic septicemia, dropsy, ulceration and asymptomatic septicemia. A. hydrophila secretes many extracellular products (ECPs) which contribute to effective infection, wide distribution and great adaptability to environmental changes. Crude ECPs of A. hydrophila CK257, a strain used in this study, exhibits a toxic activity to the animals including mouse, rabbit and fish. Toxic symptoms were indicated by tissue damage and skin injuries in animal. When ECPs were subcutaneously injected to animals, skin damages were observed, appearing like necrosis. Preliminary research demonstrated that the active factors are protein component. The crude ECPs were collected after ammonium sulfate precipitation of cell-free culture supernatant. ECPs were fractionated with the use gel filtration chromatography. Five ECP fractions were obtained, of which one fraction was found to be toxic to goldfish. MALDI-TOF analyses provided two interesting proteases called M35 and M28. Both M35 and M28 are known as metalloprotease. Accordingly, proteins in an active fraction exhibited caseinolytic activity. These proteins were difference of caseinolytic activity under different metallic ions. Also active fraction has elastolytic activity. These results suggested that peptidase M28 and M35 may be a candidate factor for tissue necrosis activity about infection with A. hydrophila.
 Keywords
Aeromonas hydrophila;metalloprotease;necrosis;
 Language
Korean
 Cited by
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