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Genetic Relationship of Some Cirsium Plants of Korea
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 2,  2015, pp.243-248
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.2.243
 Title & Authors
Genetic Relationship of Some Cirsium Plants of Korea
Bae, Young-Min;
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Cirsium chanroenicum, Cirsium nipponicum, and Cirsium schantarense plants were collected from Changwon, Ulleungdo, and Dooryoon Mountain, respectively. Cirsium japonicum plants were also collected from various locations in Korea. Genomic DNA was prepared from the collected plants and used for amplification of the 18S rDNA, ITS1, 5.8S rDNA, ITS2, and part of 28S rDNA. The ITS1 and ITS2 sequences of the PCR products and from other Cirsium plants reported previously were aligned and compared. Cirsium chanroenicum, Cirsium nipponicum, and Cirsium setidens formed distinct branches on the neighbor-joining tree. Cirsium japonicum and Cirsium pendulum appeared to be close to one another, but Cirsium pendulum plants were clearly clustered in an independent clade. Cirsium shantarense was clustered with the other Cirsium japonicum plants. The most important characteristic that distinguished these two species was the direction of the flowers. All Cirsium japonicum flowers point upward, but Cirsium shantarense flowers point downward. Other than this feature, these two species are almost indistinguishable morphologically. Cirsium chanroenicum is indistinguishable morphologically from Cirsium setidens, but it still formed a distinct group on the neighbor-joining tree based on ITS sequences, suggesting that this species is worth considering as an independent species. Silymarin production of the collected plants was analyzed and appeared to be quite high, indicating that the ability to synthesize silymarin is common to all Cirsium plants analyzed so far.
Genetic relationship;ITS;PCR;silymarin;thistle;
 Cited by
Adhikari, M., Arora, R., Chawla, R., Sharma, J., Dhaker, A. S., Gupta, D., Dubey, N., Kumar, R., Ivanov, V., Gadjeva, V., Gevrenova, R. and Sharma, R. K. 2010. Evaluation of silymarin as a promising radioprotector. Z. Naturforsch. C. 65, 337-346.

Alikaridis, F., Papadakis, D., Pantelia, K. and Kephalas, T. 2000. Flavonolignan production from Silybum marianum transformed and untransformed root cultures. Fitoterapia 71, 379-384. crossref(new window)

Chenna, R, Sugawara, H., Koike, T., Lopez, R., Gibson, T. J., Higgins, D. G. and Thompson, J. D. 2003. Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. 31, 3497-3500. crossref(new window)

Cheung, C. W., Gibbons, N., Johnson, D. W. and Nicol, D. L. 2010. Silibinin - A promising new treatment for cancer. Anticancer Agents Med. Chem. 10, 186-195. crossref(new window)

Häffner, E. and Hellwig, F. H. 1999. Phylogeny of the tribe Cardueae (Compositae) with emphasis on the subtribe Carduinae: an analysis based on ITS sequence data. Willdenowia 29, 27-39. crossref(new window)

Han, H. K., Je, H. S. and Kim, G. H. 2010. Effects of Cirsium japonicum powder on plasma glucose and lipid level in streptozotocin induced diabetic rats. Kor. J. Food Sci. Technol. 42, 343-349.

Jeong, D. M., Jung, H. A. and Choi, J. S. 2008. Comparative antioxidant activity and HPLC profiles of some selected Korean thistles. Arch. Pharm. Res. 31, 28-33. crossref(new window)

Katiyar, S. K., Mantena, S. K. and Meeran, S. M. 2011. Silymarin protects epidermal keratinocytes from ultraviolet radiation-induced apoptosis and DNA damage by nucleotide excision repair mechanism. PLoS One 6, e21410. crossref(new window)

Lee, D. G., Kim, H. K., Park, Y. K., Park, S. C., Woo, E. R., Jeong, H. G. and Hahm, K. S. 2003. Gram-positive bacteria specific properties of silybin derived from Silybum marianum. Arch. Pharm. Res. 26, 597-600 crossref(new window)

Lee, T. B. 2006. Coloured flora of Korea, 2nd ed., Hyangmoonsa: Seoul, Korea.

Polyak, S. J., Morishima, C., Lohmann, V., Pal, S., Lee, D. Y., Liu, Y., Graf, T. N. and Oberlies, N. H. 2010. Identification of hepatoprotective flavonolignans from silymarin. Proc. Natl. Acad. Sci. USA 107, 5995-5999. crossref(new window)

Song, M. J. and Kim, H. 2005. Taxonomic identities of Cirsium setidens, C. chanroenicum and C. toraiense. Kor. J. Pl. Taxon. 35, 227-245.

Song, M. J. and Kim, H. 2007. Taxonomic study on Cirsium Miller (Asteraceae) in Korea based on external morphology. Kor. J. Pl. Taxon. 37, 17-40.

Tamura, K., Nei, M., and Kumar, S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. U.S.A. 101, 11030-11035. crossref(new window)

Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol. Biol. Evol. 30, 2725-2729. crossref(new window)

Yoo, S. K. and Bae, Y. M. 2012. Phylogenetic and chemical analysis of Cirsium pendulum and Cirsium setidens inhabiting Korea. J. Life Sci. 22, 1120-1125. crossref(new window)