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Apoptotic Cell Death of Human Leukemia U937 Cells by Essential Oil purified from Schisandrae Semen
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 2,  2015, pp.249-255
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.2.249
 Title & Authors
Apoptotic Cell Death of Human Leukemia U937 Cells by Essential Oil purified from Schisandrae Semen
Choi, Yung Hyun;
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 Abstract
Schisandrae fructus [Schizandra chinensis (Turcz.) Baillon] is a medicinal herb widely used for treating various inflammatory and immune diseases in East Asian countries. The Schisandrae Semen essential oil (SSeo) from this plant has pharmacological activities, including antioxidant, antimicrobial, and antitumoral activities. Nevertheless, the biological activities and underlying molecular mechanisms of the potential anti-cancer effects of this oil remain unclear. In the present study, we investigated the potential inhibition of apoptosis signaling pathways by SSeo in human leukemia U937 cells and evaluated the underlying molecular mechanism. Exposure to SSeo resulted in a concentration-dependent growth inhibition due to apoptosis, which was verified by DNA fragmentation, the presence of apoptotic bodies, and an increase in the sub-G1 ratio. Induction of apoptotic cell death by SSeo was correlated with the down-regulation of members of the inhibitor of apoptosis protein (IAP) family (including X-linked inhibitor of apoptosis protein (XIAP), cIAP-1, and surviving) and anti-apoptotic Bcl-2, and with up-regulation of death receptor (DR) 4 and DR5, depending on dosage. SSeo treatment also induced Bid truncation, mitochondrial dysfunction, proteolytic activation of caspase-3, -8 and -9, and concomitant degradation of activated caspase-3 target proteins such as poly (ADP-ribose) polymerase. Taken together, these findings suggest that SSeo may be a potential chemotherapeutic agent for use in the control of human leukemia cells. Further studies are needed to identify its active compounds.
 Keywords
Apoptosis;caspase;essential oil;MMP;Schisandrae Semen;
 Language
Korean
 Cited by
1.
Chemical Components Composition on Different Parts of Fruit in Schisandra chinensis Baillon, Journal of the Korean Society of Food Science and Nutrition, 2016, 45, 6, 851  crossref(new windwow)
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