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Muskelin Interacts with Multi-PDZ Domain Protein 1 (MUPP1) through the PDZ Domain
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 5,  2015, pp.594-600
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.5.594
 Title & Authors
Muskelin Interacts with Multi-PDZ Domain Protein 1 (MUPP1) through the PDZ Domain
Jang, Won Hee; Jeong, Young Joo; Choi, Sun Hee; Lee, Won Hee; Kim, Mooseong; Kim, Sang-Jin; Urm, Sang-Hwa; Moon, Il Soo; Seog, Dae-Hyun;
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Protein-protein interactions have a critical role in the regulation of many cellular functions. Postsynaptic density-95/disks large/zonula occludens-1 (PDZ) domain is one of domains that mediate protein-protein interactions. PDZ domains typically bind to the specific motif at the carboxyl (C)-terminal end of partner proteins. Multi-PDZ domain protein 1 (MUPP1), which has 13 PDZ domains, serves a scaffolding function for structure proteins and signaling proteins, but the cellular function of MUPP1 has not been fully elucidated. We used the yeast two-hybrid system to identify proteins that interact with PDZ domains of MUPP1. We found an interaction between MUPP1 and muskelin. Muskelin was recently identified as a GABAA receptor (GABAAR) α1 subunit binding protein and known to have a role in receptor endocytosis and degradation. Muskelin bound to the 3rd PDZ domain, but not to other PDZ domains of MUPP1. The C-terminal end of muskelin was essential for the interaction with MUPP1 in the yeast two-hybrid assay. When co-expressed in HEK-293T cells, muskelin but not the C-terminal deleted muskelin was co-immunoprecipitated with MUPP1. In addition, MUPP1 co-localized with muskelin at the same subcellular region in cells. These findings collectively suggest that MUPP1 or its interacting proteins could modulate GABAAR trafficking and turnover through the interaction with muskelin.
Adaptor protein;MUPP1;muskelin;PDZ domain;protein-protein interaction;
 Cited by
Wdpcp, a Protein that Regulates Planar Cell Polarity, Interacts with Multi‐PDZ Domain Protein 1 (MUPP1) through a PDZ Interaction, Journal of Life Science, 2016, 26, 3, 282  crossref(new windwow)
Adachi, M., Hamazaki, Y., Kobayashi, Y., Itoh, M., Tsukita, S., Furuse, M. and Tsukita, S. 2009. Similar and distinct properties of MUPP1 and Patj, two homologous PDZ domain-containing tight-junction proteins. Mol. Cell. Biol. 29, 2372-2389. crossref(new window)

Ardura, J. A. and Friedman, P. A. 2011. Regulation of G protein-coupled receptor function by Na+/H+ exchange regulatory factors. Pharmacol. Rev. 63, 882-900. crossref(new window)

Balasubramanian, S., Fam, S. R. and Hall, R. A. 2007. GABAB receptor association with the PDZ scaffold Mupp1 alters receptor stability and function. J. Biol. Chem. 282, 4162-4171.

Becamel, C., Figge, A., Poliak, S., Dumuis, A., Peles, E., Bockaert, J., Lubbert, H. and Ullmer, C. 2001. Interaction of serotonin 5-hydroxytryptamine type 2C receptors with PDZ10 of the multi-PDZ domain protein MUPP1. J. Biol.Chem. 276, 12974-12982. crossref(new window)

Delto, C. F., Heisler, F. F., Kuper, J., Sander, B., Kneussel, M. and Schindelin, H. 2015. The LisH motif of muskelin is crucial for oligomerization and governs intracellular localization. Structure 23, 364-373. crossref(new window)

Dooley, R., Baumgart, S., Rasche, S., Hatt, H. and Neuhaus, E. M. 2009. Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens1 (PDZ) scaffold multi-PDZ domain protein 1. FEBS J. 276, 7279-7290. crossref(new window)

Doyle, D. A., Lee, A., Lewis, J., Kim, E., Sheng, M. and MacKinnon, R. 1996. Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ. Cell 85, 1067-1076. crossref(new window)

Field, C. M. and Kellogg, D. 1999. Septins: cytoskeletal polymersor signaling GTPases? Trends Cell. Biol. 9, 387-394. crossref(new window)

Garner, C. C., Nash, J. and Huganir, R. L. 2000. PDZ domainsin synapse assembly and signalling. Trends Cell. Biol. 10, 274-280. crossref(new window)

Gomperts, S. N. 1996. Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell. 84, 659-662. crossref(new window)

Guillaume, J. L., Daulat, A. M., Maurice, P., Levoye, A., Migaud, M., Brydon, L., Malpaux, B., Borg-Capra, C. and Jockers, R. 2008. The PDZ protein mupp1 promotes Gi coupling and signaling of the Mt1 melatonin receptor. J. Biol. Chem. 283, 16762-16771. crossref(new window)

Guillemot, L., Foglia, A., Paschoud, S., Pulimeno, P. and Citi, S. 2008. The cytoplasmic plaque of tight junctions: a scaffolding and signalling center. Biochim. Biophys. Acta. 1778, 601-613. crossref(new window)

Hamazaki, Y., Itoh, M., Sasaki, H., Furuse, M. and Tsukita, S. 2002. Multi-PDZ domain protein 1 (MUPP1) is concentrated at tight junctions through its possible interaction with claudin-1 and junctional adhesion molecule. J. Biol. Chem. 277, 455-461. crossref(new window)

Heisler, F. F., Loebrich, S., Pechmann, Y., Maier, N., Zivkovic, A. R., Tokito, M., Hausrat, T. J., Schweizer, M., Bähring, R., Holzbaur, E. L., Schmitz, D. and Kneussel, M. 2011. Muskelin regulates actin filament- and microtubule-based GABA(A) receptor transport in neurons. Neuron 70, 66-81. crossref(new window)

Hirbec, H., Perestenko, O., Nishimune, A., Meyer, G., Nakanishi, S. and Henley, J, M. 2002. The PDZ proteins PICK1, GRIP and Syntenin bind multiple glutamate receptor subtypes. J. Biol. Chem. 277, 15221-15224. crossref(new window)

Jang, W. H., Choi, S. H., Jeong, J. Y., Park, J. H., Kim, S. J. and Seog, D. H. 2014. Neuronal cell-surface protein neurexin 1 interaction with multi-PDZ domain protein MUPP1. Biosci. Biotechnol. Biochem. 78, 644-646. crossref(new window)

Kimber, W. A., Trinkle-Mulcahy, L., Cheung, P., Deak, M., Marsden, L. J. and Kieloch, A. 2002. Evidence that the tandem-pleckstrin-homology-domain-containingprotein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo. Biochem. J. 361, 525-536. crossref(new window)

Krapivinsky, G., Medina, I., Krapivinsky, L., Gapon, S. and Clapham, D. E. 2004. SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation. Neuron 43, 563-574. crossref(new window)

Pei, L., Teves, R. L., Wallace, M. C. and Gurd, J. W. 2001. Transient cerebral ischemia increases tyrosine phosphorylation of the synaptic RAS-GTPase activating protein, SynGAP. J. Cereb. Blood Flow Metab. 21, 955-963.

Ponting, C. P., Phillips, C., Davies, K. E. and Blake, D. J. 1997. PDZ domains: targeting signalling molecules to sub-membranous sites. Bioessays 19, 469-479. crossref(new window)

Roche, K. W., Standley, S., McCallum, J., Dune Ly, C., Ehlers, M. D. and Wenthold, R. J. 2001. Molecular determinants of NMDA receptor internalization. Nat. Neurosci. 4, 794-802. crossref(new window)

Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular cloning: a laboratory manual. Cold Spring Habor Laboratory, Cold Spring Habor, New York.

Setou, M., Nakagawa, T., Seog, D. H. and Hirokawa, N. 2000. Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport. Science. 288, 1796-1802. crossref(new window)

Setou, M., Seog, D. H., Tanaka, Y., Kanai, Y., Takei, Y., Kawagishi, M. and Hirokawa, N. 2002. Glutamate-receptorinteracting protein GRIP1 directly steers kinesin to dendrites. Nature. 417, 83-87. crossref(new window)

Sheng, M. and Sala, C. 2001. PDZ domains and the organization of supramolecular complexes. Annu. Rev. Neurosci. 24, 1-29. crossref(new window)

Songyang, Z., Fanning, A. S., Fu, C., Xu, J., Marfatia, S. M. and Chishti, A. H. 1997. Recognition of unique carboxyl-terminal motifs by distinct PDZ domains. Science 275, 73-77. crossref(new window)

Ullmer, C., Schmuck, K., Figge, A. and Luëbbert, H. 1998. Cloning and characterization of MUPP1, a novel PDZ domain protein. FEBS Lett. 424, 63-68. crossref(new window)