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Recovery from the Two-generation Reproductive Toxicity in Sprague-Dawley Rats by Treatment with Decursin and Decursinol Angelate
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  • Journal title : Journal of Life Science
  • Volume 25, Issue 7,  2015, pp.765-772
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.7.765
 Title & Authors
Recovery from the Two-generation Reproductive Toxicity in Sprague-Dawley Rats by Treatment with Decursin and Decursinol Angelate
Kim, Kang Min; Ok, Seon; Go, Youn Suk; Kang, Jae Seon;
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The aim of this study was to determine the effect of decursin (D) and decursinol angelate (DA) against bisphenol A (BPA) toxicity in a rat two-generation study. Adult rats were divided into the following three groups: (1) control, (2) BPA, and (3) BPA+D/DA. The D and DA treatment of F0 parents increased the terminal body weight and relative adult organ weights (testes, kidneys, spleen, and liver) when compared with the BPA group. A significant decrease in sperm count was found in the BPA+D/DA (7.69%) and BPA (64.70%, p<0.01) groups, when compared with the sperm count in the control group. No offspring were obtained in the F1 generation of the BPA (50 mg/kg/day) group, but the addition of D/DA in the BPA+D/DA group significantly restored fertility (55.78%) and gestation indices (98.87%) in the F1 generation. No significant differences were found in the fertility index between the control (75.02%) and the BPA+D/DA (78.11%) groups in the two-generation study, when compared with the one-generation study. The viability ratio during lactation in the D/DA group was also similar to that of the control group. These data indicate that D/DA (50 mg/kg/day) administered over two generations causes significant positive changes in reproductive or developmental parameters.
Angelica gigas Nakai;decursin;decursinol angelate;reproductive toxicity;two-generation;
 Cited by
Ahn, M. J., Lee, M. K., Kim, Y. C. and Sung, S. H. 2008. The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/massspectrometry. J. Pharmaceut. Biomed. 46, 258-266. crossref(new window)

Ahn, K. S., Sim, W. S. and Kim, I. H. 1996. Decursin: a cytotoxic agent and protein kinase C activator from the root of Angelica gigas. Planta. Med. 62, 7-9. crossref(new window)

Akhtar, N., Srivastava, M. K. and Raizada, R. B. 2009. Assessment of chlorpyrifos toxicity on certain organs in rat, Rattusnorvegicus. J. Environ. Biol. 30, 1047-1053.

Biegel, L. B., Cook, J. C., Hurtt, M. E. and O’Connor, J. C. 1998a. Effects of 17 β-estradiol on serum hormone concentrations and estrous cycle in female Crl:CD BR rats: Effects on parental and first generation rats. Toxicol. Sci. 44, 143-154.

Goodman, J. E., McConnell, E. E., Sipes, I. G., Witorsch, R. J., Slayton, T. M., Yu, C. J., Lewis, A. S. and Rhomberq, L. R. 2006. An updated weight of the evidence evaluation of reproductive and developmental effects of low doses of bisphenol A. Crit. Rev. Toxicol. 36, 387-457. crossref(new window)

Konoshima, M., Chi, H. J. and Hata, K. 1968. Coumarins from the root of Angelica gigas Nakai. Chem. Pharm. Bull. 16, 1139-1140. crossref(new window)

Kang, S. Y., Lee, K. Y., Sung, S. H., Park, M. J. and Kim, Y. C. 2001. Coumarins isolated from Angelica gigas inhibit acetylcholinesterase structure-activity relationships. J. Nat. Prod. 64, 683-685. crossref(new window)

Kim, S. J., Kim, M. R., Hwang, S. Y., Bae, W. J., Kim, S., Hong, S. H., Lee, J. Y., Hwang, T. K., Wang, Z. and Kim, S. W. 2013. Preliminary Report on the safety of a new herbal formula and its effect on sperm quality. World J. Mens Health 31, 254-261. crossref(new window)

Kim, K. M., Kim, T. H., Park, Y. J., Kim, I. H. and Kang, J. S. 2009. Evaluation of the genotoxicity of decursin and decursinol angelate produced by Angelica gigas Nakai. Mol. Cell. Toxicol. 5, 83-87.

Kim, K. M., Lee, Y. J., Hong, Y. G. and Kang, J. S. 2009. Oral acute and subacute toxicity studies of decursin and decursinol angelate of Angelica gigas Nakai. Mol. Cell. Toxicol. 5, 153-159.

Kim, K. M., Seo, J. L. and Kang, J. S. 2014. Decursin and decursinol angelate affect spermatogenesis in the adult rat at oral administration. Mol. Cell. Toxicol. 10, 83-89. crossref(new window)

Kim, K. M., Jung, J. Y., Hwang, S. W., Kim, M. J. and Kang, J. S. 2009. Isolation and purification of decursin and decursinol angelate in Angelica gigas Nakai. J. Korean Soc. Food Sci. Nutr. 38, 653-656. crossref(new window)

Lee, S. H., Lee, Y. S., Jung, S. H., Shin, K. H., Kim, B. K. and Kang, S. S. 2003. Anti-tumor activities of decursinol angelate and decursin from Angelica gigas. Arch. Pharm. Res.26, 727-730. crossref(new window)

Lee, S. H., Shin, D. S., Kim, J. S., Oh, K. B. and Kang, S. S. 2003. Antibacterial courmins from Angelica gigas roots. Arch. Pharm. Res. 26, 449-452. crossref(new window)

Liu, C., Duan, W., Li, R., Xu, S., Zhang, L., Chen, C., He, M., Lu, Y., Wu, H., Pi, H., Luo, X., Zhang, Y., Zhong, M., Yu, Z. and Zhou, Z. 2013. Exposure to bisphenol A disrupts meiotic progression during spermatogenesis in adultrats through estrogen-like activity. Cell Death Dis. 4, 1-10.

McLachlan, R. I., Wreford, N. G., O’Donnell, L., de Kretser, D. M. and Robertson, D. M. 1996. The endocrine regulation of spermatogenesis: independent roles for testosterone and FSH. J. Endocrinol. 148, 1-9. crossref(new window)

Ryu, K. S., Hong, N. D., Kim, N. J. and Kong, Y. Y. 1990. Studies on the coumarin constituents of the root of Angelicagigas Nakai. Isolation of decursinol angelate and assay of decursinol angelate and decursin. Kor. J. Pharmacogn. 21,64-68.


Reel, J. R., George, J. D., Myers, C. B., Lawton, A. D. and Lamb, I. V. 1985. Bisphenol A: reproduction and fertility assessment in CD-1 mice when administered in the feed. Final study report, NTP/NIEHS Contract No. N01-ES-2-5014, National Technical Information Service (NTIS) Accession No. PB86103207, May 3.

Šutiaková, I., Kovalkovičová, N., Tulenková, M. and Šutiak, V. 2012. Bisphenol A and its potential toxic effects on living organisms. J. Microb. Biotechnol. Food Sci. 2, 526-535.

Son, C. Y., Baek, I. H., Song, G. Y., Kang, J. S. and Kwon, K. I. 2009. Pharmacological effect of decursin and decursinol angelate from Angelica gigas Nakai. J. Kor. Pharm. Sci. 53, 303-313.

Tyl, R. W., Marr, M. C., Thomas, B. F., Keimowitz, A. R., Brine, D. R., Veselica, M. M., Fail, P. A., Chang, T. Y., Seely, J. C., Joiner, R. L., Butala, J. H., Dimond, S. S., Cagen, S. Z., Shiotsuka, R. N., Stropp, G. D. and Waechter, J. M. 2002. Three-generation reproductive toxicity study of dietary bisphenol Ain CD Sprague-Dawley rats. Toxicol. Sci. 68, 121-146. crossref(new window)

Uzun, F. G., Kalender, S. Durak, D., Demir, F. and Kalender, Y. 2009. Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E. Food Chem. Toxicol. 47, 1903-1908. crossref(new window)

WHO, 1992. Research guidelines for evaluating the safety and efficacy or herbal medicines. Regional office for the western pacific working group on the safety and efficacy on herbal medicine. pp. 5-9. Manila, Philippines.

Yook, C. S. 1990. Coloured medicinal plants of Korea, pp. 390, Academy Book Co., Seoul, Korea,

Yim, D. S., Singh, R. P., Agarwal, C., Lee, S. Y. and Chi, H. J. 2005. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res. 65, 1035-1044.