Advanced SearchSearch Tips
Dietary Intake and Accumulation of Phlorotannins in Abalone after Feeding the Phaeophyte Ecklonia stolonifera
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 25, Issue 7,  2015, pp.780-785
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.7.780
 Title & Authors
Dietary Intake and Accumulation of Phlorotannins in Abalone after Feeding the Phaeophyte Ecklonia stolonifera
Bangoura, Issa; Hong, Yong-Ki;
  PDF(new window)
Dietary intake and bioavailability of phorotannins in abalone was investigated after feeding with the phlorotannin-rich brown seaweed Ecklonia stolonifera after 4 days starvation. Reverse-phase high-performance liquid chromatography (RP-HPLC) affords isolation and quantification of the major phlorotannins of 7-phloroeckol and eckol, which were identified by mass spectrometry and nuclear magnetic resonance. Abalone growth and feed consumption rates were similar when fed either with the E. stolonifera or the common feed seaweed Saccharina japonica for 20 days. Throughout the feeding period, 7-phloroeckolol was accumulated in the abalone flesh tissue up to an average of 0.58±0.13 mg/g dry weight after 6 days. Eckol was reached to 0.25±0.05 mg/g dry tissue after 6 days, and maintained the level until end of feeding period. By feeding S. japonica as a control, no phlorotannins were detected in the abalone tissues. Both of the abalone, fed with E. stolonifera or S. japonica, had enzymes that decomposed 7-phloroeckol and eckol in muscle tissues, with similar degradation rates of −0.05 or less and −0.05 mg/ml/hr, respectively. Phlorotannins were reduced by constitutive enzymes in abalone tissues. Therefore, value-added abalone containing bioactive phlorotannins can be produced by simply changing the feed to the phlorotannin-rich brown seaweed E. stolonifera 6 days before harvest.
Abalone;accumulation;Ecklonia stolonifera;phlorotannin;
 Cited by
Fucaceae: A Source of Bioactive Phlorotannins, International Journal of Molecular Sciences, 2017, 18, 6, 1327  crossref(new windwow)
Bangoura, I., Chowdhury, M. T. H., Kang, J. Y., Cho, J. Y., Jun, J. C. and Hong, Y. K. 2014. Accumulation of phlorotannins in the abalone Haliotis discus hannai after feeding the brown seaweed Ecklonia cava. J. Appl. Phycol. 26, 967-972. crossref(new window)

Carefoot, T. H., Karentz, D., Pennings, S. C. and Young, C. L. 2000. Distribution of mycosporine-like amino acids in the sea hare Aplysia dactylomela: effect of diet on amounts and types sequestered over time in tissues and spawn. Comp. Biochem. Phys. C. 126, 91-104.

Chowdhury, M. T. H., Bangoura, I., Kang, J. Y., Park, N. G., Ahn, D. H. and Hong, Y. K. 2011. Distribution of phlorotannins in the brown alga Ecklonia cava and comparison of pretreatments for extraction. Fish. Aquat. Sci. 14, 198-204.

de Nys, R., Steinberg, P. D., Rogers, C. N., Charlton, T. S. and Duncan, M. W. 1996. Quantitative variation of secondary metabolites in the sea hare Aplysia parvula and its hostplant Delisea pulchra. Mar. Ecol. Prog. Ser. 130, 135-146. crossref(new window)

FAO. 2009. The State of World Fisheries and Aquaculture 2008, pp. 1-176, Food and Agriculture Organization of the United Nations: Rome, Italy.

Hyun, R. G., Choi, J. S. and Na, D. H. 2010. Quantitative determination of major phlorotannins in Ecklonia stolonifera. Arch. Pharm. Res. 33, 539-544. crossref(new window)

Jung, H. A., Hyun, S. K., Kim, H. R. and Choi, J. S. 2006. Angiostensins-converting enzyme I inhibitory activity of phlorotannins from Ecklonia stolonifera. Fish. Sci. 72, 1292-1299. crossref(new window)

Kang, H. S., Chung, H. Y., Kim, J. Y., Son, B. W., Jung, H. A. and Choi, J. S. 2004. Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation. Arch. Pharm. Res. 27, 194-198. crossref(new window)

Kim, A. R., Shin, T. S., Lee, M. S., Park, J. Y., Park, K. E., Yoon, N. Y., Kim, J. S., Choi, J. S., Jang, B. C., Byun, D. S., Park, N. K. and Kim, H. R. 2009. Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and anti-inflammatory properties. J. Agric. Food. Chem. 57, 3484-3489.

Kim, J. A. and Lee, J. M. 2004. The changes of biologically functional compounds and antioxidant activities in Ecklonia cava with blanching times. Kor. J. Food Cult. 19, 369-377.

Korean Fisheries Association. 2014. Korean Fisheries Yearbook, pp. 343-345, Uno Design Publishing Co.: Seoul, Korea.

Kubanek, J., Lester, S. E., Fenical, W. and Hay, M. E. 2004. Ambiguous role of phlorotannins as chemical defenses in the brown alga Fucus vesiculosus. Mar. Ecol. Prog. Ser. 277, 79-93. crossref(new window)

Okada, Y., Ishimaru, A., Suzuki, R. and Okuyama, T. A. 2004. New phloroglucinol derivative from the brown alga Eisenia bicyclis: potential for the effective treatment of diabetic complications. J. Nat. Prod. 67, 103-105. crossref(new window)

Ragan, M. A. and Glombitza, K. W. 1986. Phlorotannins, brown algal polyphenols. Prog. Phycol. Res. 4, 129-241.

Rogers, C. N., de Nys, R., Charlton, T. S. and Steinberg, P. D. 2000. Dynamics of algal secondary metabolites in two species of sea hare. J. Chem. Ecol. 26, 721-744. crossref(new window)

Smith, A. J., Robertson-Andersson, D. V., Peall, S. and Bolton, J. J. 2007. Dimethylsulfoniopropionate (DMSP) accumulation in abalone Haliotis midae (Mollusca: Prosobranchia) after consumption of various diets, and consequences for aquaculture. Aquaculture 269, 377-389. crossref(new window)

Tokuda, H., Kawashima, S., Ohno, M. and Ogawa, H. 1994. Seaweeds of Japan, pp. 106-137, Midori Shobo Co., Ltd.: Tokyo, Japan.

Troell, M., Robertson-Andersson, D., Anderson, R. J., Bolton, J. J., Maneveldt, G., Halling, C. and Probyn, T. 2006. Abalone farming in South Africa: an overview with perspectives on kelp resources, abalone feed, potential for on-farm seaweed production and socio-economic importance. Aquaculture 257, 266-281. crossref(new window)

van Dorsten, F. A., Grün, C. H., van Velzen, E. J. J., Jacobs, D. M., Draijer, R. and van Duynhoven, J. P. M. 2010. The metabolic fate of red wine and grape juice polyphenols in humans assessed by metabolomics. Mol. Nutr. Food Res. 54, 1-12.

Winter, F. C. and Estes, J. A. 1992. Experimental evidence for the effects of polyphenolic compounds from Dictyoneurum californicum Ruprecht (Phaeophyta: Laminariales) on feeding rate and growth in the red abalone Haliotus rufescens Swainson. J. Exp. Mar. Biol. Ecol. 155, 263-277. crossref(new window)