JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Characterization of Bacillus mojavensis KJS-3 for the Promotion of Plant Growth
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Life Science
  • Volume 25, Issue 8,  2015, pp.910-916
  • Publisher : Korean Society of Life Science
  • DOI : 10.5352/JLS.2015.25.8.910
 Title & Authors
Characterization of Bacillus mojavensis KJS-3 for the Promotion of Plant Growth
Kim, Kang Min; Liu, Jie; Go, Youn Suk; Kang, Jae Seon;
  PDF(new window)
 Abstract
Scientists have recently shown an interest in the characteristics of Bacillus mojavensis strains because of their increasing use in plants as a defense against diseases and mycotoxins. We have shown here that B. mojavensis KJS-3 possesses the typical characteristics of B. mojavensis strains including a strong resistance to high temperatures (≤50℃), tolerance to high salt concentrations (7% NaCl), ethanol tolerance (40% ethanol), and pH range for growth (pH 5-9). B. mojavensis KJS-3 has been used for the production of cyclic lipopeptides including important antifungal substances such as surfactin, iturin, and fengycin. Polymerase chain reaction analysis in this study showed that B. mojavensis KJS-3 can be used for the production of fengycin and the findings of LC-MS/MS analyses suggest that B. mojavensis KJS-3 can be used to produce iturin and surfactin. Antifungal activity analys is confirmed that B. mojavensis KJS-3 has antifungal effects on Botrytis cinerea, Rhizoctonia solani AG-4, Sclerotinia sclerotiorum, and Colletotricum goeosporioides. A microscopy assessment of the roots of wild ginseng plants planted together with B. mojavensis KJS-3 revealed that the roots contained B. mojavensis KJS-3, confirming the bacteria to be a plant growth promoting endophyte (PGPE) which acts against plant diseases and mycotoxins. Our findings lead us to conclude that B. mojavensis KJS-3 can be produced at an industrial level as a microbial pesticide or microbial fertilizer.
 Keywords
Antifungal activity;Bacillus mojavensis KJS-3;plant pathogenic fungi;plant growth promoting endophyte;
 Language
English
 Cited by
 References
1.
Bacon, C. W. and Hinton, D. M. 2002. Endophytic and biological control potential of Bacillus mojavensis and related species. Biol. Control 23, 274-284. crossref(new window)

2.
Bacon, C. W. and Hinton, D. M. 2007. Potential for control of seedling blight of wheat caused by Fusarium graminearum and related species using the bacterial endophyte Bacillus mojavensis. Biocontrol. Sci. Technol. 17, 81-94. crossref(new window)

3.
Bloemberg, G. V. and Lugtenberg, B. 2001. Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Curr. Opin. Plant Biol. 4, 343-350. crossref(new window)

4.
Cakmakci, R., Dönmez, F. Aydın, A. and Sahin, F. 2006. Growth promotion of plants by plant growth-promoting rhizobacteria under greenhause and two different field soil conditions. Soil. Biol. Biochem. 38, 1482-1487. crossref(new window)

5.
Choi, S. M., Park, M. H., Jung, T. S., Moon, K. H., Kim, K. M. and Kang, J. S. 2011. Characterization of Bacillus mojavensis KJS-3 for industrial applications. Arch. Pharm. Res. 34, 289-298. crossref(new window)

6.
Fernandes, P. A. V., Arruda, I. R. D., Santo, A. F. A. B. D., Araújo, A. A. D., Maior, A. M. S. and Ximenes, E. A. 2007. Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria. Braz. J. Microbiol. 38, 704-709. crossref(new window)

7.
Glick, B. R. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41, 109-117. crossref(new window)

8.
Hallmann, J., Qualt-Hallmann, A., Mahaffee, W. F. and Kloepper, J. W. 1997. Bacterial endophytes in agricultural crops. Can. J. Microbiol. 43, 895-914. crossref(new window)

9.
Jang, Y., Kim, S. G. and Kim, Y. H. 2011. Biocontrol efficacies of Bacillus species against cylindrocarpon destructans causing ginseng root rot. Plant Pathol. J. 27, 333-341. crossref(new window)

10.
Kassas, M. 1977. Arid and semi-arid lands: problems and prospects. Agro-ecosyst. 3, 185-204.

11.
Khan, M. A., Gul, B. and Weber, D. J. 2002. Improving seed germination of Salicorniarubra (Chenopodiaceae) under saline conditions using germination regulating chemicals. West. N. Am. Naturalist 62, 101-105.

12.
Kim, K. M., Jung, T. S., Ok, S., Ko, C. Y. and Kang, J. S. 2011. In vitro Characterization study of Bacillus mojavensis KJS-3 for a potential probiotic. Food Sci. Biotechnol. 20, 1155-1159. crossref(new window)

13.
Kim, K. M., Jung, T. S., Ok, S., Ko, C. Y. and Kang, J. S. 2012. Evaluation of genotoxicity of Bacillus mojavensis KJS-3 on culture supernatant for use as a probiotic. Mol. Cell. Toxicol. 8, 77-81. crossref(new window)

14.
Li, Y., Han, L., Zhang, Y., Fu, X., Chen, X., Zhang, L., Mei, R. and Wang, Q. 2013. Biological control of apple ring rot on fruit by Bacillus amyloliquefaciens 9001. Plant Pathol. J. 29, 168-173. crossref(new window)

15.
Mukherjee, A. K. and Das, K. 2005. Correlation between diverse cyclic lipopeptides production and regulation of growth and substrate utilization by Bacillus subtilis strains in aparticular habitat. FEMS Microbiol. Ecol. 54, 479-489. crossref(new window)

16.
Pyo, J. S., Shrestha, S. (Amatya), Park, S. H. and Kang, J. S. 2014. Biological control of plant growth using the plant growth-promoting rhizobacterium Bacillus mojavensis KJS-3. J. Life Sci. 24, 1308-1315. crossref(new window)

17.
Ramarathnam, R., Bo, S., Chen, Y., Fernando, W. G., Xuewen, G. and de Kievit, T. 2007. Molecular and biochemical detection of fengycin and bacillomycin D-producing Bacillus SPP., antagonistic to fungal pathogens of canola and wheat. Can. J. Microbiol. 53, 901-911. crossref(new window)

18.
Roberts, M. S., Nakumora, L. K. and Cohan, F. M. 1994. Bacillus mojavensis sp.Nov., distinguishable from Bacillus subtilis by sexual isolation, divergence in DNA sequence, and differences in fatty acid composition. Int. J. Syst. Bacteriol. 44, 256-264. crossref(new window)

19.
Ryu, H., Park, H., Suh, D. S., Jung, G. H. and Park, K. 2014. Biological control of Colletotrichum panacicola on Panax ginseng by Bacillus subtilis HK-CSM-1. J. Ginseng Res. 38, 215-219. crossref(new window)

20.
Vanittanakom, N., Loeffler, W., Koch, U. and Jung, G. 1986. Fengycin--a novel antifungal lipopeptide antibiotic produced by Bacillus subtilis F-29-3. J. Antibiot. 39, 888-901. crossref(new window)

21.
Vater, J., Kablitz, B., Wilde, C., Franke, P., Mehta, N. and Cameotra, S. S. 2002. Matrix-assisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactant in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Appl. Environ. Microb. 68, 6210-6219. crossref(new window)